A New Method for Closing Tympanic Membrane Perforations Using Basic Fibroblast Growth Factor

Hakuba, Nobuhiro; Taniguchi, Masafumi; Shimizu, Yoshitaka; Sugimoto, Akira; Shinomori, Yusuke; Gyo, Kiyofumi

doi:10.1097/00005537-200308000-00016

Cited by 65 publications

(82 citation statements)

References 16 publications

(16 reference statements)

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“…the success rate of chronic TM perforation closure, which has been reported clinically by Hakuba et al [1] . Experimental studies have indicated that bFGF is produced in situ following laceration of the TM and facilitates healing of the perforation by stimulating the proliferation and differentiation of endothelial cells, fibroblasts, and keratinocytes at the margin [7,8] .…”

Section: Disclosure Statementmentioning

confidence: 71%

“…Recently, recombinant bFGF has been used for myringoplasty [1] , and we also use bFGF myringoplasty and for ear surgery [2] . bFGF is used in myringoplasty to increase in the blood supply to the TM [9] .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, recombinant basic fibroblast growth factor (bFGF) has been used for myringoplasty [1] , and we also use bFGF for ear surgery [2] . bFGF is known to modulate, in vitro, the function of a wide variety of cells of mesodermal, neuroectodermal and endodermal origin [3,4] .…”

Section: Introductionmentioning

confidence: 99%

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Expression of Fibroblast Growth Factor Receptors 1–4 in Human Chronic Tympanic Membrane Perforation

Kakigi

Uchida²,

Nishimura³

et al. 2009

ORL

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Objective: To locate fibroblast growth factor receptor (FGFRs) 1–4 in human chronic tympanic membrane (TM) perforation. Methods: A sample of human chronic TM perforation was harvested during myringoplasty. The sample was immediately fixed in 4% paraformaldehyde and embedded in OCT compound. Immunohistochemistry was performed with FGFR 1–4 polyclonal antibodies. Results: FGFRs 1–4 were strongly and weakly expressed in the epidermal and mucosal layer of the TM perforation, respectively. Conclusions: As it is impossible to perform quantitative analysis based on the fluorescence intensity of each immunoreactivity, the presence of FGFRs 1–4 in the human chronic TM perforation is shown. The expressions of FGFRs 1–4 indicated that the clinical use of bFGF agent is useful for myringoplasty.

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Section: Disclosure Statementmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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Expression of Fibroblast Growth Factor Receptors 1–4 in Human Chronic Tympanic Membrane Perforation

Kakigi

Uchida²,

Nishimura³

et al. 2009

ORL

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“…5,[11][12][13][14] Other emerging regenerative methods for the potential treatment of chronic TM perforations either demonstrate poor efficacy or still need to be evaluated in terms of functional outcomes. [15][16][17][18][19][20][21][22][23][24][25] In vivo testing of hearing function in animal models relies on the ABR and DPOAE. [26][27][28] Auditory evoked potentials in mice are thought to be similar to that in humans, but reliance of measurement requires stability of a number of factors in …”

Section: Discussionmentioning

confidence: 99%

Functional Outcomes of Heparin-Binding Epidermal Growth Factor-Like Growth Factor for Regeneration of Chronic Tympanic Membrane Perforations in Mice

Maria

Gottlieb

Maria

et al. 2017

Tissue Engineering Part A

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We aim to demonstrate that regeneration of chronic tympanic perforations with heparin-binding epidermal growth factor-like growth factor (HB-EGF) delivered by an injectable hydrogel restored hearing to levels similar to that of nonperforated tympanic membranes. Chronic tympanic membrane perforation is currently managed as an outpatient surgery with tympanoplasty. Due to the costs of this procedure in the developed world and a lack of accessibility and resources in developing countries, there is a great need for a new treatment that does not require surgery. In this study, we show in a mouse model through measurement of auditory brainstem response and distortion product otoacoustic emissions that tympanic perforations lead to hearing loss and this can be predominantly recovered with HB-EGF treatment (5 mg/mL). Our animal model suggests a return to function between 2 and 6 months after treatment. Auditory brainstem response thresholds had returned to the control levels at 2 months, but the distortion product otoacoustic emissions returned between 2 and 6 months. We also show how the vibration characteristics of the regenerated tympanic membrane, as measured by laser Doppler vibrometry, can be similar to that of an unperforated tympanic membrane. Using the best available methods for preclinical evaluation in animal models, it is likely that HB-EGF-like growth factor treatment leads to regeneration of chronic tympanic membrane perforations and restoration of the tympanic membrane to normal function, suggesting a potential route for nonsurgical treatment.

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“…10 But that study used basic fibroblast growth factor. Our results suggest that fibroblast growth factor is not necessary to close dry perforations, because all dry perforations were closed after initial and repeat operations in the present study without this growth factor.…”

Section: Discussionmentioning

confidence: 99%

Clinical study of transcanal closure of tympanic membrane perforations using a collagen sponge

Tamae

Komune

2015

J. Laryngol. Otol.

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Materials and methods: We used an artificial dermis (Terdermis ® ), which is an atero-collagen sponge covered with a sheet of silicon.Patients: Nineteen ears of 17 patients with perforation of the tympanic membrane under various conditions, including large and wet perforations, underwent operation using this collagen sponge.Results: The success rate of closure after the initial surgery was 8/19. The overall success rate of closure after initial and re-operation was 14/19. The success rate of closure was 12/14 for small-sized perforations, 1/4 for middle-sized perforations and 1/1 for a large-sized perforation. Middle-and large-sized perforations required multiple surgeries. The success rate of closure was 11/11 for dry perforations, 3/4 for perforations with light otorrhoea and 0/4 for perforations with extensive otorrhoea.Conclusion: This surgery is a low-cost and minimally invasive surgery and has a high closure rate. This surgery is effective on small-sized, dry perforations although it can also close middle-and large-sized dry perforations.

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A New Method for Closing Tympanic Membrane Perforations Using Basic Fibroblast Growth Factor

Abstract: The study demonstrated that bFGF combined with an atelocollagen/silicone bilayer membrane is effective for the conservative treatment of tympanic membrane perforation.

Cited by 65 publications

References 16 publications

Expression of Fibroblast Growth Factor Receptors 1–4 in Human Chronic Tympanic Membrane Perforation

Expression of Fibroblast Growth Factor Receptors 1–4 in Human Chronic Tympanic Membrane Perforation

Functional Outcomes of Heparin-Binding Epidermal Growth Factor-Like Growth Factor for Regeneration of Chronic Tympanic Membrane Perforations in Mice

Clinical study of transcanal closure of tympanic membrane perforations using a collagen sponge

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