A New Method for Alveolar Bone Repair Using Extracted Teeth for the Graft Material

Nampo, Tomoki; Watahiki, Junichi; Enomoto, Akiko; Taguchi, Tomohiro; Ono, Miki; Nakano, Haruhisa; Yamamoto, Gou; Irié, Tarou; Tachikawa, Tetsuhiko; Maki, Koutaro

doi:10.1902/jop.2010.100016

Cited by 85 publications

(98 citation statements)

References 45 publications

(50 reference statements)

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“…Further studies of various calcified tissue indices including osteonectin will enable more valuable studies concerning the bone healing ability of autogenous tooth bone graft material. Nampo et al 25) compared the bone formation ability of autogenous tooth bone graft material and autogenous bone harvested from the long bone of rats in a mandibular defect area and reported that autogenous tooth bone graft material showed better bone formation compared to long bone. Studies based on autogenous tooth bone graft material as experimental and HA as control material grafted to a mandibular bony defect of mini-pigs reported that the experimental group showed better initial bone formation and histometric evaluations showed that the experimental group had better quantitative results 26) .…”

Section: Discussionmentioning

confidence: 99%

Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect

Kim¹,

Kim²,

Um³

et al. 2012

Journal of Korean Dental Science

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Section: Discussionmentioning

confidence: 99%

Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect

Kim¹,

Kim²,

Um³

et al. 2012

Journal of Korean Dental Science

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“…Since the physicochemical traits of DDM, such as low crystallinity and solubilities of Ca/P ions, are similar to those of the autogenous cortical bone 8) , our results may be explained in terms of the inorganic structure and composition of the materials 16) . It is known that the composition of DDM is similar to that of the autogenous bone in terms of the proportion of inorganic (65%) and organic (35%) substances 6,7) .…”

Section: Discussionmentioning

confidence: 64%

“…Since the development of tooth-derived bone grafting materials, reports showed that the physicochemical characteristics of autogenous demineralized dentin matrix (DDM) were similar to those of autogenous cortical bone [5][6][7][8] . Owing to this advantage, bone grafting materials from the autogenous tooth have been used actively, and their clinical applications were approved 6,[9][10][11][12][13][14][15][16] . Recently, a 5-year-long followup of DDM based on cone-beam computed tomography evaluation showed s uccessful guided bo n e regen erati on with ou t complications 17) .…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

Hyo-Jung

Hong

Kim

et al. 2017

J. Hard Tissue Biology.

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The aim of this study was to evaluate the osteogenic potential of demineralized dentin matrix (DDM) in supporting the osteogenic activity of MG-63 cells and compare it to a mixture of inorganic bovine bone and collagen (Bio-Oss Collagen ® ). Cell morphology, differentiation, adhesion, and growth were investigated via scanning electron microscopy and immunofluorescence analyses of F-actin, osteocalcin, and osteonectin. Cell adhesion and growth on the DDM as a bone graft material were more abundant, with cells adopting a flat shape and uniform distribution, than on the Bio-Oss Collagen ® at all the observation time points (i.e., 6, 12, 24, 48, 72 h and 14 days). Cell attachment and cytoskeleton organization assessed using confocal laser scanning microscopy revealed that the DDM provided better attachment with cytoplasmic propagation than the control. Immunofluorescence assays showed that the fluorescent intensities of osteocalcin and osteonectin as biomarkers of cellular differentiation were higher on the DDM than on Bio-Oss Collagen ® at 6, 12 and 24 h. We showed that the DDM had an enhanced osteogenic potential as a bone substitute for bone augmentation procedures in the dental and medical fields.

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“…Dentins contain factors important for bone regeneration, such as bone morphogenetic proteins and dentin matrix proteins 2,9) . The application of dentin for tissue engineering purposes has been introduced 10,11) and the clinical importance of using dentin as a bioactive material has been proven in several studies [12][13][14] .…”

Section: Ryohei Matsukawa Et Al: Immunohistological Observation Of Mmentioning

confidence: 99%

Immunohistological Observation of Milled Teeth in a Rat Mandibular Incisor Extraction Socket

Matsukawa

Ozawa

Kondo

et al. 2014

J. Hard Tissue Biology.

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Recycling unnecessary teeth for use as a bone grafting material should be a valuable approach to enhance the bone-forming process in alveolar bone defects and to reduce material costs. However, the process of bone formation using grafting material is not fully understood. The aim of this study was to assess the fate of material including milled teeth by immunohistological observation in a rat incisor extraction model. Freezemilled incisor material was injected into an extraction socket in a rat mandible bone. Four weeks after the operation, the mandible was sampled and frozen sections were prepared by the sticking film method, after which DMP-1 and CD68 were visualized by immunohistochemical staining. The results revealed that milled tooth particles remained around the socket at four weeks post-operation, and showed different reactions depending on the particle size. Small particles (3-10 m) were phagocytosed by CD68-positive cells, whereas bone regeneration occurred around large (30-60 m) particles. These results suggest that the injection of crushed teeth could provide an optimum scaffold and growth factors for bone formation in extraction sockets.

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A New Method for Alveolar Bone Repair Using Extracted Teeth for the Graft Material

Abstract: These results indicate that teeth may be an alternative material to autogenous bone for treating alveolar bone defects by grafting.

Cited by 85 publications

References 45 publications

Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect

Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

Immunohistological Observation of Milled Teeth in a Rat Mandibular Incisor Extraction Socket

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