2000
DOI: 10.1046/j.1365-2958.2000.01747.x
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A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors

Abstract: SummaryMalT, the transcriptional activator of the Escherichia coli maltose regulon, self-associates, binds promoter DNA and activates initiation of transcription only in the presence of ATP and maltotriose, the inducer. In vivo studies have revealed that MalT action is negatively controlled by the MalY protein. Using a biochemical approach, we analyse here the mechanism whereby MalY represses MalT activity. We show that MalY inhibits transcription activation by MalT in a purified transcription system. In vitro… Show more

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Cited by 50 publications
(79 citation statements)
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“…MalT via the same mechanism as MalY and Aes (19,20). Indeed, in all three cases the negative effector inhibits maltotriose binding and vice versa.…”
Section: An Interesting Finding Of This Work Is That Malk Inhibitsmentioning
confidence: 84%
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“…MalT via the same mechanism as MalY and Aes (19,20). Indeed, in all three cases the negative effector inhibits maltotriose binding and vice versa.…”
Section: An Interesting Finding Of This Work Is That Malk Inhibitsmentioning
confidence: 84%
“…BL21( DE3) ⌬malT220 contains a deletion of the entire malT gene (19). Plasmids pOM163 and pOM163-T38R are pET28b(ϩ) (Novagen) derivatives producing DT1 or DT1-T38R with a Strep tag at their C terminus (DT1S) (20).…”
Section: Methodsmentioning
confidence: 99%
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