A New Measuring Device for Non-Invasive Determination of Oxygen Partial Pressure and Oxygen Conductance of the Skin and Other Tissues

Niehoff, K.; Barnikol, W. K. R.

doi:10.1007/978-1-4615-4717-4_81

Cited by 8 publications

(7 citation statements)

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“…2a, P <0.05). This observation is not surprising as residual oxygen molecules are depleted not only by the cellular respiration but also by the Clark electrode oxygen consumption (37). In contrast, LLI does not consume oxygen.…”

Section: Discussionmentioning

confidence: 96%

“…2a, P <0.05). Again, this increase may be explained by the fact that the oxygen consumption of the Clark electrode significantly competes with the oxygen consumption of the skin – the electrode is ‘sucking’ oxygen through the skin (37). Therefore, diffusion of (again available) oxygen molecules through the skin is accelerated, cellular respiration is further impaired, and, in turn, estimated p tc O 2 values increase faster.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Babilas

Lamby²,

Prantl³

et al. 2008

Skin Research and Technology

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Babilas

Lamby²,

Prantl³

et al. 2008

Skin Research and Technology

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“…Compared to other monitoring systems, LLI is not affected by electrode displacement or oxygen consumption during measurement [22,27]. In addition to that, LLI measures the main determining parameter of flap viability, i.e.…”

Section: Discussionmentioning

confidence: 99%

Postoperative assessment of free skin flap viability by transcutaneous pO2 measurement using dynamic phosphorescence imaging

Geis

Schreml

Lamby

et al. 2009

Clinical Hemorheology and Microcirculation

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Background: Free flap transplantation is used more and more frequently in order to cover extensive wound defects. The basic prerequisite for successful flap salvage after flap failure is a short time interval from failure until revision. For this reason many different flap monitoring systems have been tested over the last years. But none of them has made the way into clinical routine.Objective: The aim of this clinical study was to study whether luminescence lifetime imaging (LLI) is an adequate method to assess flap viability during the postoperative period. In previous experiments LLI was proven to be a precise and non-invasive monitoring system for transcutaneous oxygen measurement.Methods: ptcO 2 of 9 patients was detected during a postoperative period of 72 hours. In all cases the transplantation was performed by the same experienced surgeon. During the first 4 hours almost constant ptcO 2 values were detected (53 ± 0.7 mmHg). During the following time intervals ptcO 2 values decreased and reached a more or less constant level after approximately 12 hours. The mean ptcO 2 decreased from 53 ± 0.7 mmHg to 39 ± 1.0 mmHg. In one case an immediate decrease of ptcO 2 below 10 mmHg was observed and a subsequently intervention was necessary to improve flap perfusion.Conclusion: In this clinical trial, perfusion dynamics after free flap transplantation as well as the detection of vascular complications were demonstrated using LLI. Based on these data, LLI seems to be a sensitive and adequate monitoring system for the evaluation of free flap viability.

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“…There is increasing interest in the use of tissue PO 2 as a monitor of critical illness [15,16,22,23,24]. The most favorable tissue in which to record this variable has yet to be determined, and candidates include the gut [25], subdermis [26], skeletal muscle [27], wound margins [11], brain [15], and bladder mucosa [28].…”

Section: Discussionmentioning

confidence: 99%

Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique

et al. 2002

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IntroductionAccurate measurement of PO 2 in biologic tissues has been of interest to both researchers and clinicians for many years [1]. For basic scientists measurement of PO 2 provides insight into the complexities of oxygen flux at the tissue level, whereas for clinicians it moves the monitoring window a step closer to the cell. PO 2 monitoring has been exploited most effectively by radiation oncologists, who have used intratumoral PO 2 measurements to plan and guide radiotherapy [2]. Many articles in the anesthesia and critical care literature report the application of different technologies designed to measure tissue PO 2 [1,[3][4][5][6][7][8][9][10][11][12][13][14], but the clinical use of PO 2 measurement has largely been limited to assessment of brain tissue [15,16].Existing technologies for measuring tissue PO 2 are either too expensive for everyday clinical use [14] or are based on polarographic principles [17], meaning that oxygen is consumed in the measurement process. In time this oxygen consumption affects the signal itself, and this effect persists as tissue PO 2 decreases, perhaps making polarographic devices less suitable for detection of tissue hypoxia. We hypothesized that a PO 2 measurement technique based on dynamic fluorescence quenching would provide a way to overcome the limitations of the current polarographic technique. We report here a head-to-head bench comparison of PO 2 measurement using polarography versus measurement using dynamic fluorescence quenching. We also present preliminary data from an animal model of tissue ischemia and hypoxia that provide FiO 2 =fractional inspired oxygen; PO 2 =partial oxygen tension. AbstractIntroduction and methods Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension (PO 2 ). We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO 2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemia-hypoxia. Results Both systems measured PO 2 accurately in the tonometer, and there was excellent correlation between them (r 2 = 0.99). The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo, the fluorescence quenching technique provided a readily recordable signal that varied as expected. Discussion Measurement of tissue PO 2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.

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A New Measuring Device for Non-Invasive Determination of Oxygen Partial Pressure and Oxygen Conductance of the Skin and Other Tissues

Cited by 8 publications

References 8 publications

Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Postoperative assessment of free skin flap viability by transcutaneous pO2 measurement using dynamic phosphorescence imaging

Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique

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A New Measuring Device for Non-Invasive Determination of Oxygen Partial Pressure and Oxygen Conductance of the Skin and Other Tissues

Cited by 8 publications

References 8 publications

Transcutaneous pO2 imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Transcutaneous pO2 imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Postoperative assessment of free skin flap viability by transcutaneous pO2 measurement using dynamic phosphorescence imaging

Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique

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Product

Resources

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Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors