Transcutaneous pO₂ imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors

Abstract

The presented method provides accurate and reproducible p(tc)O(2) values under changing microcirculatory conditions. The lack of oxygen consumption during measurement allows both a more realistic estimation of p(tc)O(2) than compared with the gold standard and permanent use in regions with critical oxygen supply.

Indicators for optical oxygen sensors

“…More recently, additional applications of luminescent oxygen sensor for diagnostics have been emerging such as the control of wound healing processes [200], the measurement of transcutaneous pO 2 [17], and of tumor hypoxia [258], to mention only some.…”

FRET excited ratiometric oxygen sensing in living tissue

“…To date this assumption has relied on various methodologies that either indirectly monitor hemodynamic (Ogawa et al 1990, Vanzetta and Grinvald 1999, Wolf et al 2007) and metabolic changes (LaManna et al 1987, Lakowicz et al 1992), or more direct sensing of O 2 using polarography (Clark 1956),Silver 1973), binding of nitroimidazole-based compounds (Koch 2002), and electron paramagnetic resonance methods (Swartz and Clarkson 1998). To improve spatial and temporal resolution, more recent advances in direct [O 2 ] sensing have relied on phosphorescence quenching dyes that linearly and reversibly change luminescence by single-photon (Kautsky and Hirsch 1931, Vanderkooi et al 1987, Papkovsky and O'Riordan 2005) or two-photon excitation (Sakadzic et al 2010), allowing intracellular (Xu et al 2001, Koo et al 2004, Park et al 2005, O'Riordan et al 2007, Zhdanov et al 2008, Dmitriev et al 2010, Koo Lee et al 2010, Wang et al 2011) or extracellular (Babilas et al 2008, Finikova et al 2008, Hynes et al 2009, Lebedev et al 2009, Ceroni et al 2011, Esipova et al 2011) measurements in biological samples. Conventionally, such dyes have been coupled with two-photon enhancing dendrimers (Brinas et al 2005) or O 2 insensitive organic reference dyes that are restricted by low luminescence lifetimes, dissimilar bleaching curves, and the requirement that each dye must be closely matched to the excitation and emission spectrum when using a single excitation source (Xu et al 2001).…”

Anaerobic vs aerobic pathways of carbonyl and oxidant stress in human lens and skin during aging and in diabetes: A comparative analysis

“…In order to evaluate their potential mechanism of formation, we have compared the results with similar determinations in skin collagen and systematically plotted the lens and skin data on the same graph using a mix of new and previously published data from our own laboratory. A key aspect of this important comparison is that the lens is an avascular cellular system with very low oxygen tension[17], while the skin is highly vascularized with its collagen being exposed to 30–40 mm Hg O 2 tension[18]. Another important aspect is that the half-lives of skin collagen and lens crystallins are very long, i.e.…”