A new macrolactin antibiotic from deep sea-derived bacteria Bacillus subtilis B5

Abstract

A new macrolactin derivate, 7-O-2'E-butenoyl macrolactin A (1), together with three known macrolactin compounds, macrolactin A (2), 7-O-malonyl macrolactin A (3) and 7-O-succinyl macrolactin A (4), was isolated from the bacterial strain Bacillus subtilis B5, which was isolated from the 3000 m deep sea sediment of the Southwest Pacific Ocean. The structures of the new compounds were assigned by spectroscopic methods including 1-D/2-D NMR and MS analysis techniques. Compounds 1 and 2 demonstrated antifungal activities against tea pathogenic fungi Pestalotiopsis theae and Colletotrichum gloeosporioides .

Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression

“…It was a newly isolated compound produced by deep-sea sediment of the Pacific Ocean. Many isolated macrolactins including epoxyl-macrolactin that have been isolated [19,20,21,22] and all macrolactins contain three separate diene structure elements.…”

“…Macrolactins, an important 24-membered macrolactones, are mainly produced by Bacillus [19,20,21,22]. In our previous study [19], a new macrolactin ( 2 ) and three known ones ( 3 – 5 ), were isolated from Bacillus subtilis B5 , a bacteria derived from the 3000-meter deep-sea sediment of the Pacific Ocean.…”

“…In our previous study [19], a new macrolactin ( 2 ) and three known ones ( 3 – 5 ), were isolated from Bacillus subtilis B5 , a bacteria derived from the 3000-meter deep-sea sediment of the Pacific Ocean. 7- O -2′ E -Butenoyl macrolactin A ( 2 ) exhibited antifungal activity against tea pathogenic fungi Pestalotiopsis theae and Colletotrichum gloeosporioides .…”

New Bis-Alkenoic Acid Derivatives from a Marine-Derived Fungus Fusarium solani H915

“…The antifungal activity against tea pathogenic fungi P. theae and C. gloeosporioides was evaluated according to a previously described method [10,11]. Petri plates were used in the test.…”

“…To date, reports on bioactive compounds against tea pathogenic fungi are limited. Previously, we identified two new macrolactins [10,11] from Bacillus subtilis B5, a bacterium isolated from the 3000 m deep sea sediment of the Pacific Ocean that exhibit antifungal activity against P. theae and C. gloeosporioides . In the present study, Fusarium solani H915, a fungal strain that originated from the mangrove sediment of the Zhangjiangkou Mangrove National Nature Reserve, was found to possess antifungal activity.…”