A New Luminol Sensitized Chemiluminescence Method for Determination of Superoxide Dismutase

Pascual, Carlos; Castillo, M. Dolores del; Romay, Cheyla

doi:10.1080/00032719208020040

Cited by 10 publications

(2 citation statements)

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“…2002;MARCUCCI, 1995;NAITO et al, 2007;NAKAJIMA et al, 2007;PAULINO et al, 2006;RUSSO et al, 2004), entre outras. Assim, devido à grande aplicabilidade da própolis, muitos estudos científicos têm sido realizados em todo mundo, chegando a totalizar mais de 800 trabalhos publicados nos últimos 20 anos (PUBMED, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi, 2007, palavra chave: própolis).…”

Section: Os Radicais Livres E O Efeito Da Radiação Uv Na Peleunclassified

“…2002;MARCUCCI, 1995;NAITO et al, 2007;NAKAJIMA et al, 2007;PAULINO et al, 2006;RUSSO et al, 2004) Com relação à comercialização da própolis, em geral os países importadores, principalmente o Japão, preferem própolis de origem brasileira a própolis de outras origens, uma vez que seus constituintes têm sido relacionados a importantes propriedades biológicas e também pelo menor teor de metais pesados e demais poluentes ambientais (PEREIRA; SEIXAS; AQUINO NETO, 2002).…”

Section: Os Radicais Livres E O Efeito Da Radiação Uv Na Peleunclassified

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Desenvolvimento de formulações tópicas fotoquimioprotetoras contendo extrato de própolis: estudos de estabilidade, permeação e retenção cutânea in vitro e de eficácia in vivo

Oliveira¹

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The ultraviolet radiation (UVR) exposition may lead to the skin oxidant/antioxidant imbalance injuring its integrity and leading to several disorders, such as ageing and skin cancer. In order to improve the biological effects caused by free radicals generated by UVR in skin, it has been suggested the photochemoprotection by using topical antioxidants. Among the available compounds to be employed in photochemoprotection, propolis, due to its important antioxidant activity, among its innumerous biological activities, is a promising topical raw-material. Next, ethanolic and glycolic propolis extracts (EPE, GPE) were characterized in relation to their polyphenolic composition, and in relation to their antioxidant activity against several free-radicals. Formulations added with these extracts were developed and undergone to physical and functional stability studies, in vitro release and skin permeation and retention studies, as well as in vivo preliminary efficacy studies. The results showed that the propolis extracts are able to scavenge several free radicals efficiently, mainly superoxide radicals. When these extracts were added to formulations of topical products, their antioxidant activity were maintained. In the physical stability studies, it was observed that the most stable formulations were developed with Hostacerin ® SAF (lower fat content) and Polawax ® (higher fat content). However, only the formulation developed with Polawax ® showed satisfactory stability for 1 year stored at room temperature and at 40º.C ± 2º.C/70%UR ± 5%UR. In the release, permeation and retention studies, it was observed the fat content influence in the formulation performance. The release profile of p-coumaric acid (used as marker compound) and the compounds equivalent to propolis extract (EPE) followed the Higuchi model. The formulation developed with Polawax ® showed the best skin retention, retaining 0,013 and 0,030 µL EPE.cm -2 in hairless mouse skin and in pig skin, respectively. In addition, this formulation presented low permeation, which is desired for photochemoprotective topical employment. In the in vivo studies, this formulation added with EPE was able to diminish erithema, inhibit oedema and increase cicatrisation in hairless mice exposed to UVB radiation. In addition, it was also observed the protection of the endogenous glutathione (GSH) depletion. The in vivo preliminary efficacy results suggest that formulations added with propolis extract present good perspectives to be employed to prevent and treat the injuries caused in skin by UV radiation.Keywords: Propolis, Antioxidant Activity, Photochemoprotection, Topical Formulations, Percutaneous absorption. Figura 7 -Compartimento de madeira contendo a fonte de luz UVB utilizado nos experimentos de indução de estresse oxidativo na pele de camundongos sem pêlo. 1: tampa superior; 2: lâmpada UVB; 3: tampa frontal; 4: local para ventilação; 5: acionamento da lâmpada UVB. 61Figura 8 -Representação da avaliação da formação do eritema nos animais expostos à radiação UVB por 2, 4...

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Section: Os Radicais Livres E O Efeito Da Radiação Uv Na Peleunclassified

Desenvolvimento de formulações tópicas fotoquimioprotetoras contendo extrato de própolis: estudos de estabilidade, permeação e retenção cutânea in vitro e de eficácia in vivo

Oliveira¹

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Effect of silymarin and silybinin on oxygen radicals

Pascual¹,

Gonz²,

Armesto³

et al. 1993

Drug Development Research

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The aim of this work was to study the scavenging action of the flavonoids, silymarin and silybinin (two well known hepatoprotective agents) against different types of oxygen radicals which were generated by specific chemical reactions and detected by lurninol-sensitized chemilurninescence. A well known scavenger for each radical was used as control for the paradigm and for comparing its effect with those of the flavonoids. It was found that 0.8 pg/ml of silyrnarin and 5.5 mg/ml of silybinin produced the same effect as 3.2 ng/ml superoxide dismutase (SOD). An alkoxy radical scavenging effect similar to the one produced by 0.35 ng/ml of vitamin E was caused by 2 pg/ml silyrnarin and 1.6 nglrnl silybinin. Silybinin was evaluated as a scavenger of hydroxyl radicals by determining the inhibition of the chemiluminescence produced by the Fenton reaction with luminol; this was compared with the results obtained from a more conventional method which determines the inhibition of the damage to 2-deoxyribose by the Fenton reaction. In both methods, inhibition was observed at a relatively high concentration of the flavonoid as compared with ethanol. The action of silyrnarin and silybinin as scavengers of oxygen radicals may explain the protective effect of these flavonoids in certain forms of liver disease where free radicals are involved. However, other properties of these compounds cannot be discarded for their hepatoprotective actions. 0 1993 Wiley-Liss, Inc.

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Antioxidant Assays

Charles¹

2012

Antioxidant Properties of Spices, Herbs and Other Sources

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A New Luminol Sensitized Chemiluminescence Method for Determination of Superoxide Dismutase

Cited by 10 publications

References 13 publications

Desenvolvimento de formulações tópicas fotoquimioprotetoras contendo extrato de própolis: estudos de estabilidade, permeação e retenção cutânea in vitro e de eficácia in vivo

Desenvolvimento de formulações tópicas fotoquimioprotetoras contendo extrato de própolis: estudos de estabilidade, permeação e retenção cutânea in vitro e de eficácia in vivo

Effect of silymarin and silybinin on oxygen radicals

Antioxidant Assays

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