Antioxidant Assays

Charles, Denys J.

doi:10.1007/978-1-4614-4310-0_2

Cited by 17 publications

(30 citation statements)

References 224 publications

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Section: Antioxidant Capacity Total Phenolic and Total Flavonoid Conmentioning

confidence: 99%

“…All of these methods are usually used to determine the antioxidant features of a sample, even though their action mechanisms are different. DPPH and ABTS measure the antiradical capacity of the sample, while the FRAP assay determines its reducing capacity [32]. According to the antioxidant capacity results, it would seem that both the ethanol concentration in the medium as well as the ratio solid/solvent have an influence on the extraction of antioxidant compounds from grape stems ( Figure 2).…”

Section: Antioxidant Capacity Total Phenolic and Total Flavonoid Conmentioning

confidence: 99%

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Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts

et al. 2019

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The use of grape stems for the extraction of bioactive compounds to be used in the pharmaceutical, food, and cosmetic industries is a promising objective. The aim of this work is to determine the influence of the different extraction conditions (temperature, ethanol concentration, and ratio of sample/solvent) on phenolic composition and antioxidant capacity of Mazuelo stem extracts. In general, the ethanol concentration of the extraction solvent was the factor that had the greatest influence on the extraction of different bioactive compounds. The greatest content of total phenolic compounds and the highest antioxidant activity of the extracts were obtained with 50% ethanol and at 40 °C. The most abundant compound found in the different extracts obtained from Mazuelo grape stem was (+)-catechin, but appreciable concentrations of gallic acid, a quercetin derivative, and stilbenes (trans-resveratrol and trans-ε-viniferin) were also extracted. Quercetin and malvidin-3-glucoside showed the highest correlation with the antioxidant capacity of the extracts, while stilbenes did not present such relation. The maximum concentration of gallic acid was extracted with water but the extraction of most of the compounds was maximum on using 50% ethanol. Consequently, the selection of the extraction method to be used will depend on the particular compound to be extracted in greatest quantity.

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Section: Antioxidant Capacity Total Phenolic and Total Flavonoid Conmentioning

confidence: 99%

Section: Antioxidant Capacity Total Phenolic and Total Flavonoid Conmentioning

confidence: 99%

Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts

et al. 2019

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“…In addition to being utilized directly as food, mustard seeds are used to add heat and a complexity of flavor. Mustard seed consumption was found to stimulate salivary secretion in human subjects and thus acts as a digestive stimulant [ 5 , 112 ], this explains their use as an appetizer in Asian, and Chinese cultures. In addition, mustards stimulate the pancreatic lipase and amylase secretion in experimental rats [ 118 ].…”

Section: Use Of Mustards As Foodmentioning

confidence: 99%

“…Literature suggests that, within the history of human settlement in Australia and New Zealand, different types of Brassicaceae mustards, namely Alliaria petiolata , Brassica alba , B. carinata , B. juncea , B. napus , B. nigra , B. rapa , Calepina irregularis , Erysimum repandum , Neslia paniculata , Sisymbrium officinale , S. orientale and S. erysimoides have been naturalized ( Figure 1 ), and adapted for use as food, incorporated into traditional medicine and play an important role in the agriculture of the two countries [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Brassicaceae Mustards: Traditional and Agronomic Uses in Australia and New Zealand

et al. 2018

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Commonly cultivated Brassicaceae mustards, namely garlic mustard (Alliaria petiolata), white mustard (Brassica alba), Ethiopian mustard (B. carinata), Asian mustard (B. juncea), oilseed rape (B. napus), black mustard (B. nigra), rapeseed (B. rapa), white ball mustard (Calepina irregularis), ball mustard (Neslia paniculata), treacle mustard (Erysimum repandum), hedge mustard (Sisymbrium officinale), Asian hedge mustard (S. orientale), smooth mustard (S. erysimoides) and canola are the major economically important oilseed crops in many countries. Mustards were naturalized to Australia and New Zealand and Australia is currently the second largest exporter of Brassicaceae oilseeds to meet the global demand for a healthy plant-derived oil, high in polyunsaturated fats. Apart from providing edible oil, various parts of these plants and many of their phytochemicals have been used traditionally for both agronomic as well as medicinal purposes, with evidence of their use by early Australian and New Zealand settlers and also the indigenous population. This review provides an overview of the current knowledge of traditional and agronomic uses of Brassicaceae oilseeds and mustards with a focus on their importance in Australia and New Zealand.

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“…Oxidative stress is a signal that reflects the overwhelming production of reactive oxygen and nitrogen species (RONS) that exceeds the antioxidant capacity within a cell. Prolonged oxidative stress can damage cellular building blocks, disrupt cellular signaling, and induce the release of inflammatory signaling molecules [ 3 , 4 ]. Indeed, RONS-mediated oxidative stress has been found in the initiation, development, and progression of cancer.…”

Section: Introductionmentioning

confidence: 99%

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Al-Nemari

Al-Senaidy

Semlali

et al. 2020

BMC Complement Med Ther

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Background The research and application of plants in food supplements and drugs have attracted great interest. This study aimed to examine the efficiency of several solvents for the extraction of the main compounds from Annona squamosa leaves and to evaluate the antioxidant, antibacterial, and anticancer activities of these extracts. Methods Gas chromatography-mass spectrometry was used to screen the bioactive compounds of A. squamosa methanolic extract. The free radical, hydrogen peroxide, and nitric oxide scavenging activities of the extracts were investigated. Furthermore, MTT, nuclear staining, LDH, and monolayer wound repair assays were performed to evaluate the potential anticancer activity of the extracts in colon cancer cells while the antibacterial activity was tested by using a well diffusion assay. Results A. squamosa leaves extracts were found to contain several bioactive compounds, of which the majority were sesquiterpenes (C15H24). These extracts exhibited strong antioxidant activity and antibacterial potency against both gram-positive and gram-negative bacteria. Different A. squamosa leaves extracts displayed remarkable antiproliferative, cytotoxic, antimigration, and apoptotic activities in colon cancer cells. Conclusions A. squamosa leaves contain major bioactive compounds that inhibit the growth of several types of bacteria and colon cancer cell lines, which demonstrated their efficacy as an alternative source of antibiotics and for the development of novel drugs for colon cancer therapy.

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Antioxidant Assays

Cited by 17 publications

References 224 publications

Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts

Impact of Extraction Conditions on the Phenolic Composition and Antioxidant Capacity of Grape Stem Extracts

Brassicaceae Mustards: Traditional and Agronomic Uses in Australia and New Zealand

GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

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