A new lignan and active compounds inhibiting NF-κB signaling pathway from Caulis Trachelospermi

In vivo TNF- and IL-1 inhibitory activity of phenolics isolated from Trachelospermum jasminoides (Lindl.) Lem

“…Furthermore, our findings provide evidence of the bioactivity of the EAF and the tested compounds in inflammatory diseases and suggest that they may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators. Our results are in accordance with the previously reported results for the mechanism of action of trachelogenin, nor-trachelogenin and tracheloside, apigenin, quercetin and luteolin for exerting their antiinflammatory activities through modulation of the cytokine system (Jia et al, 2014;Seo et al, 2014;Zhu et al, 2013;Harrison and Cooper, 2008;Chen et al, 2004;Ueda, 2004;Xagorari et al, 2001;Habtemariam et al, 2000).…”

“…Bioactive metabolites flavonoids, lignans, sterols and triterpenes, alkaloids were reported in the plant (Zhang et al, 2013;Zhu et al, 2013;Xing-qi et al, 2006;Tan et al, 2005;Jing et al, 2005;Atta-ur-Rahman et al, 1988;Fatima et al, 1987). The plant is reputed for its anti-inflammatory, analgesic activities and its anti cancer activities (Li et al, 2003;Nishibe et al, 1987).…”

Active Components with Inhibitory Activities on IFN-γ/STAT1 and IL-6/STAT3 Signaling Pathways from Caulis Trachelospermi

“…Initial investigation for new active herbal extract with inhibiting activity on JAK/STAT signaling pathway revealed that the extract of Caulis Trachelospermi, which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography, was founded to strongly inhibit IFN-γ-induced STAT1-responsive luciferase activity (IFN-γ/STAT1) with IC 50 value of 2.43 μg/mL as well as inhibiting IL-6-induced STAT3-responsive luciferase activity (IL-6/STAT3) with IC 50 value of 1.38 μg/mL in vitro (Table 2). In subsquent screening for high active components from the extract, lignan compounds 1 – 4 together with other lignan compounds isolated in previous study [7,8,9,10] were first tested in concentration of 5 μM which was close to the IC 50 value of the extract. Following result showed aglycone compounds trachelogenin and arctigenin had high inhibition ratio more than 30% (77.5% and 32.6%) on IFN-γ/STAT1 pathway as well as aglycone compounds trachelogenin, arctigenin and matairesinol inhibiting IL-6/STAT3 pathway with inhibition ratio of 96.1%, 44.2% and 89.8%, respectively.…”

“…Chemical investigations indicated that it mainly contained lignans, flavonoids and triterpenoids [4,5,6]. In our previous study, the extract of Caulis Trachelospermi, which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography, exhibited marked anti-inflammatory activity in animal model [7] as well as moderate inhibiting activity on NF- κ B signaling pathway induced by TNF-α [8], its active chemical investigation led to the discovery of 30 compounds including 19 dibenzylbutyrolactone lignans [7,8,9,10,11] and its main components, trachelogenin, nortrachelogenin and matairesinol, showed corresponding moderate inhibiting activities on TNF-α/NF- κ B pathway [8]. The JAK (Janus kinase)/STAT (signal transducer and activator of transcription) pathway is another signaling pathway responsible for signal transduction of a large number of cytokines [12].…”

Recent advances in research on lignans and neolignans

“…Arisanschinins F-L (97)(98)(99)(100)(101)(102)(103), characterized from the fruits of the Taiwanese medicinal plant Schisandra arisanensis, showed moderate in vitro inhibition of proliferation of peripheral blood mononuclear cells (PBMC) induced by phytohemaglutinin (PHA). 43 The EtOAc extract of fruits of Schisandra chinensis (Turcz.)…”