A new LC–MS/MS method for multiple residues/contaminants in bovine meat

Hajrulai-Musliu, Zehra; Uzunov, Risto; Jovanov, Stefan; Jankuloski, Dean; Stojkovski, Velimir; Pendovski, L.; Sasanya, James Jacob

doi:10.1186/s13065-021-00788-5

Cited by 19 publications

(12 citation statements)

References 28 publications

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“…The global contamination of food products encompasses various substances, including microorganisms and their nitrates, nitrites, hormones, pesticide residues, antibiotics, metabolites, mycotoxins, heavy metals, dioxins, polychlorinated biphenyls (PCBs), genetically modified organisms (GMOs), toxic pigments and melamine [ 13 ]. Residues of drugs can arise from the therapeutic or prophylactic use of medications in animals raised for food [ 14 ]. The main veterinary drugs that have the potential to contaminate food products include antimicrobial drugs, sedative drugs, nonsteroidal anti-inflammatory drugs (NSAIDs), anticoccidials, growth promoters and anti-helminthic [ 15 ].…”

Section: Regulatory Overview Of Drug Residues In Foodmentioning

confidence: 99%

“…LC-MS/MS is the most widely used method for detecting drug residues in food. HPLC-MS/MS technology has gained significant interest due to its quick response time, excellent sensitivity, high accuracy, good separation capability, positive/negative ion mode compatibility and broad applicability in analyzing complex samples like swine, bovine, poultry eggs and chicken muscle, aquatic products, meat and milk [ 14 , 83 , 87 , 96 , 96 ]. The high sensitivity and specificity of MS detectors enable the application of generic sample preparation procedures, allowing for the simultaneous extraction of a wide range of residues with different properties.…”

Section: Analysis Of Drug Residues In Foodmentioning

confidence: 99%

“…Clenbuterol, amoxicillin and clenbuterol-d6 were the banned analyte, analyte with permitted limits and internal standard, respectively. The MRM parameter includes all + ESI: for ionisation mode, 276.97, 367.07 and 283.03 m/z ; for precursor ion, 131.87, 167.77 and 202.95; 90.89, 159.94 and 203.56 m/z for product ion; 16, 30 and 30, and 16, 40 and 16 V of collision energy; 22, 28 and 22 V cone voltage [ 14 ].…”

Section: Analysis Of Drug Residues In Foodmentioning

confidence: 99%

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Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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The presence of drug residues in food products has become a growing concern because of the adverse health risks and regulatory implications. Drug residues in food refer to the presence of pharmaceutical compounds or their metabolites in products such as meat, fish, eggs, poultry and ready-to-eat foods, which are intended for human consumption. These residues can come from the use of drugs in the field of veterinary medicine, such as antibiotics, antiparasitic agents, growth promoters and other veterinary drugs given to livestock and aquaculture with the aim of providing them as prophylaxis, therapy and for promoting growth. Various analytical techniques are used for this purpose to control the maximum residue limit. Compliance with the maximum residue limit is very important for food manufacturers according to the Food and Drug Administration (FDA) or European Union (EU) regulations. Effective monitoring and control of drug residues in food requires continuous advances in analytical techniques. Few studies have been reviewed on sample extraction and preparation techniques as well as challenges and future directions for the determination of veterinary drug residues in food. This current review focuses on the overview of regulations, classifications and types of food, as well as the latest analytical methods that have been used in recent years (2020–2023) for the determination of drug residues in food so that appropriate methods and accurate results can be used. The results show that chromatography is still a widely used technique for the determination of drug residue in food. Other approaches have been developed including immunoassay, biosensors, electrophoresis and molecular-based methods. This review provides a new development method that has been used to control veterinary drug residue limit in food.

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Section: Regulatory Overview Of Drug Residues In Foodmentioning

confidence: 99%

Section: Analysis Of Drug Residues In Foodmentioning

confidence: 99%

Section: Analysis Of Drug Residues In Foodmentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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“…Currently, more sensitive methods for the determination of residues and contaminants in food of animal origin have been developed, but there are few multi-residue and multiclass analytical methods for simultaneous detection of residues of veterinary drugs and other hazards such us pesticides and mycotoxins. Zhan et al, (2013) [ 9 ], developed an LC–MS/MS method for screening for multi-class veterinary drug residues and other contaminants in muscle while Hajrulai-Musliu et al, (2021), reported an LC–MS/MS method for multiple residues and contaminants in bovine meat [ 10 ]. Danezis et al, (2016) [ 11 ], developed a HILIC chromatography-MS/MS method for detection of pesticides, plant hormones, veterinary drugs and mycotoxins in various food matrices while Gómez-Pérez et al, (2015) [ 12 ], published a method for analysis of pesticide and veterinary drug residues in baby food by liquid chromatography coupled to Orbitrap high resolution mass spectrometry, as well as Wei et al, (2015) [ 13 ], who developed multi-residue screening method for analysis of veterinary drugs, their metabolites and pesticides in meat by LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Multi-class/residue method for determination of veterinary drug residues, mycotoxins and pesticide in urine using LC-MS/MS technique

Hajrulai-Musliu,

Uzunov,

Jovanov

et al. 2023

BMC Vet Res

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Background Veterinary drugs are widely used in animals to prevent diseases and are a complex set of drugs with very different chemical properties. Multiclass and multi-residue methods for simultaneous detection of residues from veterinary drugs and contaminants in urine are very rare or non-existent. Therefore, the aim of this study was to develop and validate a sensitive and reliable quantitative LC-MS/MS method for simultaneous determination of a wide range of veterinary drug and pesticide residues and mycotoxins in bovine urine. This involved 42 veterinary drug residues (4 thyreostats, 6 anabolic hormones, 2 lactones, 10 beta agonists, 15 antibiotics, 5 sulphonamides), 28 pesticides and 2 mycotoxins. Stable isotopically labelled internal standards were used to facilitate effective quantification of the analytes. Analysis was performed in both positive and negative ionization modes with multiple reaction monitoring transitions over a period of 12 min. Results The parameters validated included linearity, limit of detection (LOD), limit of quantification (LOQ), detection capability (CCβ), decision limit (CCα), stability, accuracy and precision. The process followed guidelines of the regulation 2021/808/EC. The calibration curves were linear with coefficient of correlation (R2) from 0.991 to 0.999. The LODs were from 0.01 to 2.71 µg/L, while the LOQs were from 0.05 to 7.52 µg/L. The CCα and CCβ were in range 0.05–12.11 µg/L and 0.08–15.16 µg/L. In addition, the average recoveries of the spiked urine samples were from 71.0 to 117.0% and coefficient of variation (CV) < 21.38% (intraday and interday). Conclusion A new isotopic LC-MS/MS method has been developed, validated and applied for identification and quantification of 72 residues of veterinary drugs and pesticides and other contaminants such as mycotoxins in bovine urine. The most appropriated sample preparation procedures involved sodium acetate buffer, enzymatic hydrolysis using β-glucuronidase and cleanup solid phase extraction with OASIS SPE cartridges. The parameters were satisfactorily validated fulfilling requirements under Regulation 2021/808/EC. Consequently, the method could be used in routine analysis of bovine urine samples for simultaneous detection of veterinary drug and pesticide residues as well as contaminants such as mycotoxins.

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“…A low-temperature cleanup method has been developed for these challenging drug residue and contaminant detections in which most lipid components can be successfully separated from extracts. While a wide range of analytes were included, sulfonamides, and several pesticides were not ( 11 , 23 , 27 ).…”

Section: Introductionmentioning

confidence: 99%

Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry

Hajrulai-Musliu

Uzunov

Jovanov

et al. 2022

Journal of Veterinary Research

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Introduction Multi-class and multi-residue analyses are very complex procedures because of the physico-chemical properties of veterinary drug residues and other contaminants. The purpose of the study was to develop an analytical method for the sensitive determination of 69 analytes in bovine milk by liquid chromatography electrospray ionisation–tandem mass spectrometry. Material and Methods Antimicrobial, anabolic hormone, lactone, β-agonist, mycotoxin and pesticide residues were analysed in 120 raw milk samples from different dairy farms in North Macedonia. Stable isotopically labelled internal standards were used to facilitate effective quantification of the analytes. Results The linear regression coefficients were higher than 0.99, the limits of detection ranged from 0.0036 to 47.94 μg/L, and the limits of quantification ranged from 0.053 to 59.43 μg/L. The decision limit values ranged from 0.062 to 211.32 μg/L and the detection capability from 0.080 to 233.71 μg/L. Average recoveries of the analytes spiked in raw milk were in the range of 70.83% to 109%, intra-day coefficient of variation (CV) values from 2.41% to 22.29%, and inter-day CV values from 3.48% to 23.91%. The method was successfully applied in the testing of bovine milk samples. In five samples residues were detected. They were sulfadimethoxine (in two samples), enrofloxacin, tetracycline and oxytetracycline and were at concentrations below the EU maximum residue limit. Conclusion The method is useful for routine testing for this group of chemical hazards in bovine milk.

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A new LC–MS/MS method for multiple residues/contaminants in bovine meat

Cited by 19 publications

References 28 publications

Recent Advances in the Determination of Veterinary Drug Residues in Food

Recent Advances in the Determination of Veterinary Drug Residues in Food

Multi-class/residue method for determination of veterinary drug residues, mycotoxins and pesticide in urine using LC-MS/MS technique

Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry

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