A New <i>Phomopsis</i> Species from Soybean

Hobbs, Thomas; Schmitthenner, A. F.; Kuter, Geoffrey A.

doi:10.1080/00275514.1985.12025139

Cited by 57 publications

(27 citation statements)

References 10 publications

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“…Our isolates were phylogenetically identical to others identified as such. Moreover, the morphology of our isolates correlated entirely with the original description of this species as given by Hobbs et al (1985). The absence of beta-conidia in the isolates used in the present study correlates with the scarcity of this type of spores as reported by Hobbs et al (1985).…”

Section: Discussionsupporting

confidence: 88%

“…In fact, the amount of variation in the size of perithecia, ascospores, pycnidia, alpha- and beta-conidia reported within each taxon at least equals the variation between them (Kulik 1984). Almost at the same time, Hobbs et al (1985) considered the type specimen of Phomopsis glycines to be morphologically the same as P. sojae , and included this binomial to the list of synonyms of D. phaseolorum .…”

Section: Discussionmentioning

confidence: 99%

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Resolving the Diaporthe species occurring on soybean in Croatia

Santos

Vrandečić²,

Ćosić³

et al. 2011

persoonia

160

166

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Diaporthe (anamorph = Phomopsis) species are plant pathogens and endophytes on a wide range of hosts including economically important crops. At least four Diaporthe taxa occur on soybean and they are responsible for serious diseases and significant yield losses. Although several studies have extensively described the culture and morphological characters of these pathogens, their taxonomy has not been fully resolved. Diaporthe and Phomopsis isolates were obtained from soybean and other plant hosts throughout Croatia. Phylogenetic relationships were determined through analyses of partial translation elongation factor 1-alpha (EF1-α) gene and ITS nrDNA sequence data. By combining morphological and molecular data, four species could be distinguished on soybeans in Croatia. Diaporthe phaseolorum is described in this study and its synonyms are discussed. Diaporthe phaseolorum var. caulivora is raised to species status and the name Diaporthe caulivora is introduced to accommodate it. A species previously known as Phomopsis sp. 9 from earlier studies on sunflower, grapevine, rooibos and hydrangea is reported for the first time on soybean, and is formally described as Diaporthe novem. The well-known soybean pathogen Phomopsis longicolla was also collected in the present study and was transferred to Diaporthe longicolla comb. nov. The presence of these species on herbaceous hosts raises once more the relevance of weeds as reservoirs for pathogens of economically important plants.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Resolving the Diaporthe species occurring on soybean in Croatia

Santos

Vrandečić²,

Ćosić³

et al. 2011

persoonia

160

166

View full text Add to dashboard Cite

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“…firmed on the basis of mycological descriptions (19,24,30). Primers made to the conserved sequences of nuclear ribosomal DNA amplified the internal transcribed spacer (ITS) regions of D. phaseolorum var.…”

Section: Methodsmentioning

confidence: 99%

Using PCR to Distinguish Diaporthe phaseolorum and Phomopsis longicolla from Other Soybean Fungal Pathogens and to Detect Them in Soybean Tissues

Zhang¹,

Hartman

Riccioni

et al. 1997

Plant Disease

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Restriction fragment length polymorphism analyses of polymerase chain reaction (PCR) amplified DNA were used to distinguish Diaporthe phaseolorum and Phomopsis longicolla isolates from other soybean fungal pathogens. Primers made to the conserved sequences of nuclear ribosomal DNA amplified the internal transcribed spacer (ITS) regions of D. phaseolorum var. meridionalis and P. longicolla. The PCR products were cloned and then sequenced. Specific-primers, Phom.I and Phom.II, were designed from the polymorphic regions of D. phaseolorum and P. longicolla isolates from soybean to distinguish them from other soybean fungal pathogens. These ITS-derived primers amplified a 337-bp-specific DNA fragment from P. longicolla, D. phaseolorum var. meridionalis, D. phaseolorum var. caulivora, D. phaseolorum var. sojae, and Phomopsis spp. from 20 different hosts. No amplified product was observed using DNA of seven other soybean fungal pathogens or soybean DNA. The detection limit of PCR using primers Phom.I and Phom.II was 2.5 × 10-7 dilution of fungal DNA extracted from samples of 10 pooled seeds and as low as a 1:15 (Phomopsis:soybean) ratio when using 10 ng of DNA per μl from each P. longicolla and soybean. PCR did not produce products using primers Phom.I and Phom.II with DNA extracted from noninfected seeds, but specific bands were observed from samples of 10 pooled seeds and from individually infected seeds. A specific band was observed as well from DNA extracts of tissue samples from symptomless plants inoculated with P. longicolla and D. phaseolorum var. sojae.

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“…Putative colonies of P. longicolla were verifi ed by growing isolates on APDA, and identifi cation was based on a single-monocondial isolate using morphological and cultural characteristics (Hobbs et al, 1985;Morgan-Jones, 1989;Mengistu et al, 2007b). Seeds were disinfested as described above.…”

Section: Seed Harvesting and Disease Ratingmentioning

confidence: 99%

“…Seeds were disinfested as described above. The Phomopsis/Diaporthe isolates were identifi ed using DNA sequencing of an intron containing a portion of the translation elongation factor gene (Hobbs et al, 1985;Schilder et al, 2005;Mengistu et al, 2007bMengistu et al, , 2009). Putative colonies of P. longicolla were verifi ed by growing isolates on APDA, and identifi cation was based on a single-monocondial isolate using morphological and cultural characteristics (Hobbs et al, 1985;Morgan-Jones, 1989;Mengistu et al, 2007b).…”

Section: Seed Harvesting and Disease Ratingmentioning

confidence: 99%

Irrigation and Time of Harvest Effects on Evaluation of Selected Soybean Accessions Against Phomopsis longicolla

et al. 2010

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Phomopsis seed decay (PSD) of soybean (Glycine max L. Merr) is primarily caused by Phomopsis longicolla. Currently, percent seed infection is used as a method for evaluating resistance to PSD. However, researchers need a common system for classifying soybean genotypes for their reaction to P.longicolla that is consistent across environments. The purpose of this research was: (i) to evaluate the reaction of a select set of soybean accessions to P. longicolla, and (ii) determine the effects of irrigation and two harvest regimes (normal and delayed) on seed infection. We propose a classification system for comparing reactions to P. longicolla among accessions based on a Phomopsis seed infection index (PSII), derived by dividing the percent seed infection of each accession by percent seed infection values of susceptible standards selected within early and late maturity groups. Percent seed infection by P. longicolla infection was greater when accessions were irrigated during the growing season and harvested at normal maturity. Some lines such as MO/PSD‐0259 were rated as resistant to P. longicolla (7% seed infection) when not irrigated and harvested at maturity, but were rated as susceptible (39% seed infection) with irrigation and delayed harvest. The resistance ratings to P. longicolla using percent seed infection and PSII varied among accessions. SS93–6012, for example, had similar ratings for both (9% seed infection and 16% PSII). PI 416942 exhibited some resistance to P. longicolla based on percent seed infection (8%), but no resistance based on PSII (32%). Accessions PI 594478, Delmar, SS93–6012, SS93–6181, PI 594603A, and PI 594712 were classified by PSII as moderately resistant under irrigation and with delayed harvest. These results indicate that simple comparisons of percent seed infection among accessions without irrigation, without delayed harvest, and without a standard control of a similar maturity group might result in false conclusions about resistance to P. longicolla.

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A New Phomopsis Species from Soybean

Cited by 57 publications

References 10 publications

Resolving the Diaporthe species occurring on soybean in Croatia

Resolving the Diaporthe species occurring on soybean in Croatia

Using PCR to Distinguish Diaporthe phaseolorum and Phomopsis longicolla from Other Soybean Fungal Pathogens and to Detect Them in Soybean Tissues

Irrigation and Time of Harvest Effects on Evaluation of Selected Soybean Accessions Against Phomopsis longicolla

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