A new <i>in vitro</i> model for the study of microbial microleakage around dental restorations: a preliminary qualitative evaluation

“…They often utilized conventional methods of cavity preparation and restorations before incubating tooth specimens with chemical solutions or bacterial cultures [Kidd, 1976;Gilmour et al, 1990;Donly and Gomez, 1994;Sepet et al, 1995;Dionysopoulos et al, 1996;Fontana et al, 1996a;Matharu et al, 2001;Lobo et al, 2005;Seemann et al, 2005;Borczyk et al, 2006]. The in vitro model developed for this study provided further standardization of the gap size and specimens (i.e.…”

Section: Discussionmentioning

confidence: 99%

A New in vitro Model to Study the Relationship of Gap Size and Secondary Caries

et al. 2007

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Objectives: To investigate the relationship of gap size and secondary caries in a newly developed secondary caries microbial model that permits adjustment of the gap between the tooth and a restoration. Methods: Tooth-resin-matrix composite specimens were mounted on gap-model stages with a gap size of 50 or 500 µm in experiment 1, and 0, 25, 250, or 1,000 µm in experiment 2. They were attached to plastic Petri plates, gas-sterilized and then incubated in a microbial caries model (with Streptococcus mutans TH16 in 1% sucrose tryptic soy broth for 1 h, 4 times/day, and with a buffer solution for the rest of the day). After 8 days of incubation, tooth specimens were sectioned and stained overnight with a rhodamine B solution. Digital images taken under a confocal microscope were analyzed for lesion size at the outer surface lesion and wall lesion (WL). Results: Gap size was found to affect the development of dentin WL area in experiment 1 and enamel and dentin WL areas in experiment 2, with bigger lesions being observed in the wider gap group (p < 0.05). Conclusion: The findings of this study suggest that the size of the gap between tooth and restoration affects the development of secondary caries along the cavity wall.

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“…Discs were mounted, placed in a Polaron E5 100 sputter coater (Quorum Technologies, East Sussex, UK) and coated with gold-palladium. Images were obtained using a Stereoscan 90B electron microscope (Cambridge Instruments Ltd., Cambridge, UK) [11].…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Soluble phosphate glasses: in vitro studies using human cells of hard and soft tissue origin

et al. 2004

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A new in vitro model for the study of microbial microleakage around dental restorations: a preliminary qualitative evaluation

Abstract: This preliminary investigation showed that the CDFF may be a valuable tool for the in vitro study of the dynamics of microbial microleakage around dental restorations.

Cited by 20 publications

References 21 publications

Analysis of four gutta‐percha techniques used to fill mesial root canals of mandibular molars

Analysis of four gutta‐percha techniques used to fill mesial root canals of mandibular molars

A New in vitro Model to Study the Relationship of Gap Size and Secondary Caries

Soluble phosphate glasses: in vitro studies using human cells of hard and soft tissue origin

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