A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes

Abstract

There is a likely impact of FN3K and FN3K-RP on the glycation cascade in vivo with potential positive and negative effects. The new screening method enables further studies to elucidate the function and importance of FN3K-RP.

Frontiers in research on the Maillard reaction in aging and chronic disease

“…Among the original research articles, Scheijen and Schalkwijk describe advances in methodology for measurement of Maillard reaction intermediates in plasma by LC/MS [11], and Hellwig et al describe a novel HPLC assay for measurement of fructosamine-3-kinase, the only enzyme known to reverse Maillard reaction damage to proteins in vivo [12]. Tanhäuserová et al present an interesting analysis of genetic variability in this and other enzymes that provide protection against hyperglycemia and Maillard reaction damage to proteins in diabetes [13].…”