A new high-performance liquid chromatographic method with evaporative light scattering detector for the analysis of phospholipids. Application to Iberian pig subcutaneous fat

Narváez-Rivas, Mónica; Gallardo, Emerenciana; Ríos, José Julián; León‐Camacho, Manuel

doi:10.1016/j.chroma.2011.03.067

Cited by 32 publications

(21 citation statements)

References 28 publications

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“…For the HPLC-MS/MS method published by Lee et al 35 (phospholipids in soybean cultivars) and for the SPE-HPLC-ELSD method published by Narvaéz et al 36 (phospholipids in the subcutaneous fat of Iberian pig) the glycerophospholipid LOD/LOQ values were in the low micrograms per kilogram range. For a recently published RP-HPLC-UV method 37 (fatty acid composition of soybean PC), the values were in the low milligrams per liter range.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Comparison and Characterization of Soybean and Sunflower Lecithins Used for Chocolate Production by High-Performance Thin-Layer Chromatography with Fluorescence Detection and Electrospray Mass Spectrometry

Krüger

Bürmann

Morlock

2015

J. Agric. Food Chem.

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The scarce availability of nongenetically modified soybeans on the world market represents a growing problem for food manufacturers. Hence, in this study the effects of substituting soybean with sunflower lecithin were investigated with regard to chocolate production. The glycerophospholipid pattern of the different lecithin samples was investigated by high-performance thin-layer chromatography fluorescence detection (HPTLC-FLD) and by HPTLC-positive ion electrospray ionization mass spectrometry (ESI(+)-MS) via the TLC-MS Interface and by scanning HPTLC-matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS). Especially, the contents of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were of interest due to the influencing effects of these two glycerophospholipids on the rheological parameters of chocolate production. The lecithin substitution led to only slight differences in the rheological parameters of milk and dark chocolate. Limits of detection (LODs) and limits of quantification (LOQs) of seven glycerophospholipids were studied for three detection modes. Mean LODs ranged from 8 to 40 mg/kg for HPTLC-FLD and, using a single-quadrupole MS, from 10 to 280 mg/kg for HPTLC-ESI(+)-MS as well as from 15 to 310 mg/kg for HPTLC-FLD-ESI(+)-MS recorded after derivatization with the primuline reagent.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Comparison and Characterization of Soybean and Sunflower Lecithins Used for Chocolate Production by High-Performance Thin-Layer Chromatography with Fluorescence Detection and Electrospray Mass Spectrometry

Krüger

Bürmann

Morlock

2015

J. Agric. Food Chem.

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“…Recently, one dimension HILIC–ESI–MS method has been used for analysis of phospholipids, good separation of lysophopholipids was achieved, while co-elution of other different lipid classes, like PS and PE, was also observed [31]. Although its performance has greatly improved over other HILIC-based lipid separations, its capability in complete separation of phospholipid classes is less desirable compared with a normal phase column, where no co-elution was achieved using a Chromolith–HPLC–ESI–MS [32]. Another disadvantage of using HILIC is that most of the methods in the literature are not applicable for the quantification of nonpolar lipid classes due to their elution in the void volume [33].…”

Section: Resultsmentioning

confidence: 99%

Comprehensive untargeted lipidomic analysis using core–shell C30 particle column and high field orbitrap mass spectrometer

Narváez-Rivas

Zhang

2016

Journal of Chromatography A

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126

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The goal of untargeted lipidomics is to have high throughput, yet comprehensive and unambiguous identification and quantification of lipids. Novel stationary phases in LC separation and new mass spectrometric instruments capable of high mass resolving power and faster scanning rate are essential to achieving this goal. In this work, 4 reversed phase LC columns coupled with a high field quadrupole orbitrap mass spectrometer (Q Exactive HF) were thoroughly compared using complex lipid standard mixture and rat plasma and liver samples. A good separation of all lipids was achieved in 24 min of gradient. The columns compared include C30 and C18 functionalization on either core–shell or totally porous silica particles, with size ranging from 1.7 to 2.6 μm. Accucore C30 column showed the narrowest peaks and highest theoretical plate number, and excellent peak capacity and retention time reproducibility (<1% standard deviation). As a result, it resulted in 430 lipid species identified from rat plasma and rat liver samples with highest confidence. The high resolution offered by the up-front RPLC allowed discrimination of cis/trans isomeric lipid species, and the high field orbitrap mass spectrometer afforded the clear distinction of isobaric lipid species in full scan MS and the unambiguous assignment of sn-positional isomers for lysophospholipids in MS/MS. Taken together, the high efficiency LC separation and high mass resolving MS analysis are very promising tools for untargeted lipidomics analysis.

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“…Previous studies successfully applied HPLC-MS for phospholipids analysis [15-19]. The class separation enabled sensitive detection of phospholipids and extended the application of mass spectrometry to various biological sources.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive approach to the quantitative analysis of mitochondrial phospholipids by HPLC–MS

Kim

Hoppel

2013

Journal of Chromatography B

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A normal-phase HPLC-MS method was established to analyze mitochondrial phospholipids quantitatively as well as qualitatively. An efficient extraction procedure and chromatographic conditions were developed using twelve standardized phospholipids and lysophospholipids. The chromatographic conditions provided physical separation of phospholipids by class, and efficient ionization allowed detection of low abundance phospholipids such as phosphatidylglycerol and monolysocardiolipin. The chromatographic separation of each class of phospholipid permitted qualitative identification of molecular species without interference from other classes. This is advantageous for mitochondrial lipidomics because the composition of mitochondrial phospholipids varies depending on tissue source, pathological condition, and nutrition. Using the method, seven classes of phospholipids (phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, cardiolipin, and monolysocardiolipin) were detected in rat heart and skeletal muscle mitochondria and all but phosphatidylserine were quantified. The concentration was calculated using standard curves with an internal standard generated for each class of phospholipid. The method was validated for intraday and interday variation and showed excellent reproducibility and accuracy. This new method, with each step documented, provides a powerful tool for accurate quantitation of phospholipids, a basic structural component of mitochondrial membranes.

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A new high-performance liquid chromatographic method with evaporative light scattering detector for the analysis of phospholipids. Application to Iberian pig subcutaneous fat

Cited by 32 publications

References 28 publications

Comparison and Characterization of Soybean and Sunflower Lecithins Used for Chocolate Production by High-Performance Thin-Layer Chromatography with Fluorescence Detection and Electrospray Mass Spectrometry

Comparison and Characterization of Soybean and Sunflower Lecithins Used for Chocolate Production by High-Performance Thin-Layer Chromatography with Fluorescence Detection and Electrospray Mass Spectrometry

Comprehensive untargeted lipidomic analysis using core–shell C30 particle column and high field orbitrap mass spectrometer

Comprehensive approach to the quantitative analysis of mitochondrial phospholipids by HPLC–MS

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