A New Helicobacter pylori Vacuolating Cytotoxin Determinant, the Intermediate Region, Is Associated With Gastric Cancer

Rhead, Joanne L.; Letley, Darren P.; Mohammadi, Marjan; Hussein, Nawfal R.; Mohagheghi, Mohammad Ali; Hosseini, Mojtaba; Atherton, John C.

doi:10.1053/j.gastro.2007.06.056

Cited by 363 publications

(492 citation statements)

References 53 publications

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“…Amplification cycles were performed with Cyclogene Thermal Cycler (Techne, England), as mentioned elsewhere. 5,[7][8][9] For each batch of PCR reactions a positive internal control amplifying the human housekeeping gene glyceraldehydes-3-phosphate dehydrogenase (GAPDH), 12 and a negative control were included. PCR products were analyzed in parallel with a DNA MW-marker (Fermentas) by electrophoresis on 2% agarose gel.…”

Section: Methodsmentioning

confidence: 99%

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Helicobacter pylori genotypes among patients in a university hospital in Egypt: identifying the determinants of disease severity

Amer¹

2013

Jmid

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Objective: To detect the various of H. pylori genotypes, determine the most prevalent one, and to identify the determinants of disease severity. Methods:Biopsies from 90 patients were collected, however 69 were exclusively analyzed. Recognition of H. pylori was made by rapid urease test, histopathology and polymerase chain reaction. The latter was used to amplify genes encoding for virulence markers such as CagA, vacAs1 and vacAs2.Results: cagA was identified in 45 isolates (65.2%), vacAs1 in 54 (78.3%, predominantly vacAs1a), vacAs2 in 15 (21.7%), vacAm1 in 37 (53.6%), vacAm2 in 32 (46.4%), vacAi1 in 36 (52.2%), and vacAi2 in 32 (46.4%) of strains. vacAi1 gene was the only independent marker of pathogenicity. cagA/vacAs1(a)i1m1 was the most prevalent genotype. Sonuç: m1 ve m2 alelleri ve i1 ve i2 alellerinin çok yakın eşit dağılımları ile vacAs tipleri içinde vacAs1(a) genine sahip olan izolatlar en baskın alelik varyant idi. Çalışılan suşların büyük çoğunluğu cagA/vacAs1(a)i1m1 genotipinde idi. Hastalığın ciddiyetini gösteren tek tek belirteç vacAi1 idi. Conclusion

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Section: Methodsmentioning

confidence: 99%

“…Each of these polymorphic regions has two main types/alleles that divide them further into type/allele 1 and type/allele 2. 5 Within the 's1' type, several subtypes are distinguished (s1a, s1b and s1c). The 's1' type has more ability to form membrane channels than 's2' type.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori genotypes among patients in a university hospital in Egypt: identifying the determinants of disease severity

Amer¹

2013

Jmid

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“…The vacuolating cytotoxin A (vacA) often present in every H pylori strain (Cover et al, 1994). Variation in levels of cytotoxicity is attributed to vacA allelic variation among H pylori strain, which occurs in the signal (s), middle (m) and intermediate (i) regions, each with two different alleles (Atherton et al, 1995;van Doorn et al, 1998): s1 or s2, m1 or m2,and 1i or i2, respectively (Rhead et al, 2007). Recently, a fourth disease-related region between the i and m regions has been indentified and named the deletion (d) region.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori vacA d1 Genotype Predicts Risk of Gastric Adenocarcinoma and Peptic Ulcers in Northwestern Iran

Basiri¹,

Safaralizadeh²,

Bonyadi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: There is a close relationship between Helicobacter pylori (H pylori)-specific factors and different gastroduodenal diseases. The present study aimed to investigate the prevalence of vacA d1, d2 genotypes in the H pylori isolates from patients with gastric adenocarcinoma, peptic ulcer disease (PUD) and gastritis in East Azerbaijan region, where the incidence of gastric cancer (GC) is high. Strains isolated from this area are likely to be of European ancestry. Materials and Methods: In this study, genotyping of the vacA d region of 115 isolates obtained from patients with different gastrodoudenal diseases was accomplished by PCR methods. In addition to PCR amplification of H pylori 16S rDNA, rapid urease tests or histological examination were used to confirm the presence of H pylori in biopsy specimens. Data were collected and analyzed using SPSS version 19. Results: Of the total of 83 H pylori isolates, 36 (43.4%) contained the d1 allele and 47 (56.6%) were subtype d2. The results of the multiple linear/logistic regression analysis showed high correlation between allele d1 and gastric adenocarcinoma or PUD. Conclusions: This study suggests that the H pylori vacA d1 genotype helps predict risk for gastric adenocarcinoma and PUD in East Azerbaijan, Iran.

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“…44 The gene consists of three variable regions: the signal region (s-encoding the signal peptide), with two alleles, s1 (subtypes s1a, s1b, s1c) and s2; the medium region (m), with the alleles m1 and m2; and the intermediate region (i), containing the alleles i1 and i2. 44,45 The combination of alleles of the s, m and I regions determines the vacuolating cytotoxin production and is associated with the pathogenicity of the bacterium. In general, the strains containing s1/m1/i1 alleles produce large amounts of vacuolating cytotoxin, reason where by this genotype seems to be associated with more severe pathologies, as peptic ulcer and gastric cancer.…”

Section: Influence Of Cytokines Genes Polymorphisms In the Gastric DImentioning

confidence: 99%

Genes de patogenicidade de Helicobacter pylori, polimorfismos de citocinas e fatores ambientais afetam o desenvolvimento de doenças gástricas: uma visão geral

Vianna¹,

Silva²,

Ramis³

2016

Rev Epidemiol Control Infect

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Exceto onde especificado diferentemente, a matéria publicada neste periódico é licenciada sob forma de uma licença Creative Commons -Atribuição 4. pylori possui um conjunto de fatores de patogenicidade, tais como cagA, vacA, iceA, babA, para colonizar a mucosa gástrica e estabelecer infecção crônica. Estes fatores bacterianos são agentes essenciais em modular a resposta imune envolvida na iniciação da carcinogênesegástrica. Os fatores genéticos do hospedeiro contribuem para regular a resposta inflamatória e para o agravamento da lesão da mucosa gástrica uma vez que a infecção gástrica por H. pylori induz a produção de várias citocinas pró e anti-inflamatórias no hospedeiro. O papel prejudicial dos fatores ambientais está relacionado com as precárias condições socioeconômicas, com o consumo de sal, com o tabagismo e com o consumo de álcool. Conclusão: Ao decifrar as regras deterministas -se houver -dessa interação entre fatores da bactéria, do hospedeiro e variáveis ambientais, será possível prever, tratar e, finalmente, prevenir graves doenças gastroduodenais. Background and Objectives:Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of approximately 50% of the world´s human population. This microorganismis the major causal agent of gastritis and is an important risk factor for the development of peptic ulcer disease and gastric carcinoma. The factors that determine these diverse clinical outcomes are subject to continuous investigations and is thought to be determined by interaction of bacterial factors, host immune system and environmental variables. The aim of this review is to provide an overview of these factors that influence susceptibility to severe outcomes of H. pylori

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A New Helicobacter pylori Vacuolating Cytotoxin Determinant, the Intermediate Region, Is Associated With Gastric Cancer

Cited by 363 publications

References 53 publications

Helicobacter pylori genotypes among patients in a university hospital in Egypt: identifying the determinants of disease severity

Helicobacter pylori genotypes among patients in a university hospital in Egypt: identifying the determinants of disease severity

Helicobacter pylori vacA d1 Genotype Predicts Risk of Gastric Adenocarcinoma and Peptic Ulcers in Northwestern Iran

Genes de patogenicidade de Helicobacter pylori, polimorfismos de citocinas e fatores ambientais afetam o desenvolvimento de doenças gástricas: uma visão geral

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