Abstract:Objective: To detect the various of H. pylori genotypes, determine the most prevalent one, and to identify the determinants of disease severity.
Methods:Biopsies from 90 patients were collected, however 69 were exclusively analyzed. Recognition of H. pylori was made by rapid urease test, histopathology and polymerase chain reaction. The latter was used to amplify genes encoding for virulence markers such as CagA, vacAs1 and vacAs2.Results: cagA was identified in 45 isolates (65.2%), vacAs1 in 54 (78.3%, predom… Show more
“…Our results revealed m2 as the main allele that similar to studies from Iraq, Turkey, Iran, Saudi Arabia, and the middle east region characterized m2 as the main allele [14,32,33,[39][40][41][42]. In another hand, some studies reported that the predominant vacA m subtype was m2 and no association or no role between vacA m genotypes and the development of peptic ulcer [2,32,33,42,43], whereas other studies had been found that vacA m1 strains are associated with high levels of inflammation in the gastric mucosa and increased the risk of gastric atrophy and carcinoma [2,44]. However, the results were different from country to others, but we consistent with different studies those of Middle Eastern countries, in which the vacA s1 and m2 subtypes have been predominant subtypes [32,42,45,46].…”
Section: Resultssupporting
confidence: 90%
“…The strains in the present study belong to western type, as studies indicated s1b subtype is rarely found in East-Asian countries [29,32,38]. Our results revealed m2 as the main allele that similar to studies from Iraq, Turkey, Iran, Saudi Arabia, and the middle east region characterized m2 as the main allele [14,32,33,[39][40][41][42]. In another hand, some studies reported that the predominant vacA m subtype was m2 and no association or no role between vacA m genotypes and the development of peptic ulcer [2,32,33,42,43], whereas other studies had been found that vacA m1 strains are associated with high levels of inflammation in the gastric mucosa and increased the risk of gastric atrophy and carcinoma [2,44].…”
Section: Resultssupporting
confidence: 89%
“…However, the presence vacA gene within these isolates was increasing the pathogenicity and it had a statistically positive correlation with the disease (p=0.01 at a level 0.05). In comparison with the other studies that performed in the middle east region like Turkey and Egypt, which found vacAs1a allele is dominant (70.1% and 72.5%, respectively) than vacAs1b allele (2.8% and 7.2%, respectively) and 27.1% and 21.7%, respectively, as vacA s2 [32,33]. The allelotype s1a is more active than s1b [29], and with other vacA genotypes, the combination is associated with greater pathogenicity and virulence related to gastric cancer and peptic ulceration [34,35].…”
Objectives: Helicobacter pylori infections have been associated with the genetic diversity of their virulence factors; the virulence genotypes are valuable as a molecular marker in the diagnosis of patients with bacterial infections. Our main objective was to analyze the frequency and allelic genotype of vacuolating cytotoxin A (vacA) and cytotoxin-associated gene A (cagA) as well as investigate other virulence genes like outer inflammatory protein A gene (oipA) and a gene contact with the epithelium (iceA) of H. pylori.
Methods:A total of 75 patients with gastritis and peptic ulcer diseases (PUDs) were selected to investigate H. pylori infections. 75 antrum biopsies were collected from these patients, and then genomic DNA was extracted from biopsies using the genomic DNA kit. Subsequently, the virulence genes of H. pylori were amplified using specific primers for vacA, cagA, cagE, iceA, and oipA by polymerase chain reaction (PCR).Results: A high prevalence of genes cagA (28.6%), vacAs1bm2 (44.68%), iceA2 (30.6%), and oipA (42.9%) was found, while vacA s2m1 and iceA1 genotypes was not found in our study. There was a significant correlation between the presence of cagA and cagE genotypes (p=0.02), suggesting that these two genes almost used together as a cagPAI integrity marker and the predominant cagA EPIYA motif was ABC (~650 bp) belong to Western cagA strains. The presence of the cagA + and vacA + genes was significantly associated with peptic ulceration (p≤0.001 at a level 0.01 and p≤0.044 at a level 0.05, respectively), whereas different iceA2 genotype was no statistically significant with clinical outcome. Patients with PUD more likely to have an oipA gene (61.9%) than those with gastritis (38.1%), p≤0.037, also the presence oipA gene was statistically significant with presence iceA2 and cagA, as well as the presence iceA gene was statistically significant with the presence vacA. Conclusion: Most H. pylori genotypes which associated with peptic ulcer and gastritis were moderate virulent strains, whereas the virulent strain which associated with peptic ulcer belong to Western cagA strains had vacAs1bm2 genotype, oipA and iceA2 genes that rarely induced gastric cancer in the middle region of Iraq.
“…Our results revealed m2 as the main allele that similar to studies from Iraq, Turkey, Iran, Saudi Arabia, and the middle east region characterized m2 as the main allele [14,32,33,[39][40][41][42]. In another hand, some studies reported that the predominant vacA m subtype was m2 and no association or no role between vacA m genotypes and the development of peptic ulcer [2,32,33,42,43], whereas other studies had been found that vacA m1 strains are associated with high levels of inflammation in the gastric mucosa and increased the risk of gastric atrophy and carcinoma [2,44]. However, the results were different from country to others, but we consistent with different studies those of Middle Eastern countries, in which the vacA s1 and m2 subtypes have been predominant subtypes [32,42,45,46].…”
Section: Resultssupporting
confidence: 90%
“…The strains in the present study belong to western type, as studies indicated s1b subtype is rarely found in East-Asian countries [29,32,38]. Our results revealed m2 as the main allele that similar to studies from Iraq, Turkey, Iran, Saudi Arabia, and the middle east region characterized m2 as the main allele [14,32,33,[39][40][41][42]. In another hand, some studies reported that the predominant vacA m subtype was m2 and no association or no role between vacA m genotypes and the development of peptic ulcer [2,32,33,42,43], whereas other studies had been found that vacA m1 strains are associated with high levels of inflammation in the gastric mucosa and increased the risk of gastric atrophy and carcinoma [2,44].…”
Section: Resultssupporting
confidence: 89%
“…However, the presence vacA gene within these isolates was increasing the pathogenicity and it had a statistically positive correlation with the disease (p=0.01 at a level 0.05). In comparison with the other studies that performed in the middle east region like Turkey and Egypt, which found vacAs1a allele is dominant (70.1% and 72.5%, respectively) than vacAs1b allele (2.8% and 7.2%, respectively) and 27.1% and 21.7%, respectively, as vacA s2 [32,33]. The allelotype s1a is more active than s1b [29], and with other vacA genotypes, the combination is associated with greater pathogenicity and virulence related to gastric cancer and peptic ulceration [34,35].…”
Objectives: Helicobacter pylori infections have been associated with the genetic diversity of their virulence factors; the virulence genotypes are valuable as a molecular marker in the diagnosis of patients with bacterial infections. Our main objective was to analyze the frequency and allelic genotype of vacuolating cytotoxin A (vacA) and cytotoxin-associated gene A (cagA) as well as investigate other virulence genes like outer inflammatory protein A gene (oipA) and a gene contact with the epithelium (iceA) of H. pylori.
Methods:A total of 75 patients with gastritis and peptic ulcer diseases (PUDs) were selected to investigate H. pylori infections. 75 antrum biopsies were collected from these patients, and then genomic DNA was extracted from biopsies using the genomic DNA kit. Subsequently, the virulence genes of H. pylori were amplified using specific primers for vacA, cagA, cagE, iceA, and oipA by polymerase chain reaction (PCR).Results: A high prevalence of genes cagA (28.6%), vacAs1bm2 (44.68%), iceA2 (30.6%), and oipA (42.9%) was found, while vacA s2m1 and iceA1 genotypes was not found in our study. There was a significant correlation between the presence of cagA and cagE genotypes (p=0.02), suggesting that these two genes almost used together as a cagPAI integrity marker and the predominant cagA EPIYA motif was ABC (~650 bp) belong to Western cagA strains. The presence of the cagA + and vacA + genes was significantly associated with peptic ulceration (p≤0.001 at a level 0.01 and p≤0.044 at a level 0.05, respectively), whereas different iceA2 genotype was no statistically significant with clinical outcome. Patients with PUD more likely to have an oipA gene (61.9%) than those with gastritis (38.1%), p≤0.037, also the presence oipA gene was statistically significant with presence iceA2 and cagA, as well as the presence iceA gene was statistically significant with the presence vacA. Conclusion: Most H. pylori genotypes which associated with peptic ulcer and gastritis were moderate virulent strains, whereas the virulent strain which associated with peptic ulcer belong to Western cagA strains had vacAs1bm2 genotype, oipA and iceA2 genes that rarely induced gastric cancer in the middle region of Iraq.
“…Similar to previous studies in Turkey, Iraq, Iran, Saudi Arabia (Hussein, 2010) and China (Chung et al, 2010), m2 was predominantly found in all studied H. pylori strains (70%) previously reported. However, this was inconsistent with previous results obtained from Egypt where equal distribution of vacA m1 and m2 was found (Amer et al, 2013). Genetic variation within virulence factors may account for differences in the pathogenic properties of strains, and thus may help to explain the discrepancies between the number of infected individuals and those that end up developing gastric cancer (Ferreira et al, 2014).…”
Section: Discussioncontrasting
confidence: 76%
“…This similarity in H. pylori genotypes in three neighboring countries, Egypt, Jordan, and Gaza strip, indicates a geographic influence, which was reported by Abu Amra (2010). Whereas in Egypt, Amer et al (2013), reported that all possible combination of vacA s1 with m were recognized in their work, in addition, H. pylori virulence could not be predicted in relation to different genotypes.…”
Helicobactor pylori (H. pylori) has been strongly associated with gastritis, peptic ulcer and is linked to an increased risk of gastric cancer. The cytotoxin-associated gene product (cagA) and the vacuolating cytotoxin (vacA) have been implicated as two major virulence factors of H. pylori. Since there is an increasing evidence that genetic variability of H. pylori may have clinical importance, we aimed to evaluate different vacA genotypes and reveal its relationship with endoscopic and transmission electron microscopy (TEM) findings among H. pylori infected Egyptian patients. Forty H. pylori infected patients possessing vacA gene who underwent upper endoscopic examination were considered to be infected with H. pylori when rapid urease test and detection of 16S rRNA in gastric biopsy recorded positive. Both vacA and cagA genotypes were detected by polymerase chain reaction (PCR). The TEM was performed to assess the ultra-structure of the gastric mucosa. Four vacA genotypes were identified, the most prominent was the s2/m2 allele combination (52.5%) followed by s1/m1 (27.5%), s1/m2 (17.5%) and s2/m1 genotype was found just in one H. pylori strain (2.5%). There were significant correlations between vacA s2/m2 and gastritis (65.2%), and vacA s1/m1 and peptic ulceration (57%). The cagA gene was associated with 38% of vacA genotypes and 60% of which were significantly associated with vacA s1/m1 genotype with the development of severe gastritis reaching up to gastric ulcer. The TEM revealed H. pylori spiral and coccoid forms, cytoplasmic vacuolar degeneration caused by vacA, swollen mitochondria and dilated rough endoplasmic reticulum. In Egypt where prevalence of H. pylori infection is high, genotyping of H. pylori virulence factors can help to predict patients who are at a high risk of related gastroduodenal diseases. Although H. pylori with vacA s2/m2 genotype is mostly related to low level of virulent strains yet, significant crosstalk between H. pylori strains harboring both vacA s1/m1 and cagA gene provides crucial insights into virulence of high level.
This work aims to estimate the prevalence of Helicobacter pylori ureA gene and evaluate cagA gene-positive strains in both patients of laryngeal squamous cell carcinoma (LSCC) and those with benign laryngeal polyps. This study included 49 patients confirmed pathologically to have LSCC and 15 patients with benign laryngeal polyps over a period from June 2013 to March 2015. Samples of laryngeal tissue were collected during direct laryngoscope under general anesthesia to be pathologically evaluated followed by analysis for H. pylori detection. Each laryngeal tissue sample was divided into three parts; one for bacteriological examination, the second for pathological examination and the third for PCR to detect both ureA and cagA genes. Out of 49 LSCC samples, 31 (64.6 %) was positive for ureA by PCR. Out of them, 29 samples (93.5 %) were cagA positive. Only three cases (20 %) of the benign laryngeal polyp were ureA positive by PCR and one of them was cagA positive by PCR. By the bacteriological culture, only eight samples (25.8 %) gave growth. All of them were ureA positive and only seven of them were cagA positive. There was a significant association between presence of H. pylori and LSCC as compared to benign laryngeal polyp which may contribute in the pathogenesis of laryngeal carcinoma. These results should be confirmed by further studies over larger number of cases.
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