A new flow cytometric method for simultaneous measurement of residual platelets and RBCs in plasma: validation and application for QC

Abstract

This newly developed method provides a simple, quick, precise, and easily reproducible tool for simultaneous measurement of residual platelets and RBCs in fresh plasma.

Flow cytometric assay for the simultaneous determination of residual white blood cells, red blood cells, and platelets in fresh‐frozen plasma: validation and two years' experience

“…The counting of residual cells must be performed before freezing the plasma component. Several analytical methods have been proposed to measure residual cells in freshly prepared plasma, 3‐6 but to our knowledge no method has shown the enumeration of all three subpopulations simultaneously from the same specimen in a single‐tube test.…”

A flow cytometric method for detection and enumeration of low‐level, residual red blood cells in platelets and mononuclear cell products

“…With their particular preparation methods, this counting method was adequate for standard PLTs (1.8 × 10 9 ± 2.59 × 10 9 rRBCs per 42‐mL unit) and apheresis PLTs (0.36 × 10 9 ± 0.35 × 10 9 rRBCs per 390‐mL unit) 8 . Other groups, again usually concentrating on plasma, have reported on flow cytometric methods of counting low numbers of rRBCs in blood products with claimed linearity ranges of 3 to 54,000 RBCs per µL and sensitivities between 3 and 250 RBCs per µL 9‐11 …”

“…8 Other groups, again usually concentrating on plasma, have reported on flow cytometric methods of counting low numbers of rRBCs in blood products with claimed linearity ranges of 3 to 54,000 RBCs per µL and sensitivities between 3 and 250 RBCs per µL. [9][10][11] Continual improvements in blood component collection and preparation systems has resulted in products that are below the linear range of standard hematology analyzers. The manufacturers and users of these devices are interested in having a measurement method sensitive enough to allow determination of device performance and process capability even if the level of cross-cellular contamination is well below the regulatory recommendations.…”

Leukocyte Reduction in Blood Component Supply: The Impact of Flow Cytometry in Assessing Residual Leukocytes

“…Besides the determination of leukocytes in blood components, the simultaneous or subsequent analysis of other cellular components or contaminants such as erythrocytes and platelets will become an attractive tool [30,31]. RBCs are usually stained by using antibodies directed against glycophorin A, whereas platelets are visualized by anti-CD42.…”