Flow cytometric assay for the simultaneous determination of residual white blood cells, red blood cells, and platelets in fresh‐frozen plasma: validation and two years' experience

Abstract

These data demonstrate that the single-tube test is suitable for routine QC assessment of the cellular contaminants of therapeutic plasma according to the European recommendations.

All plasma products are not created equal

“…35 Our results varied significantly depending on the method used. 35 Our results varied significantly depending on the method used.…”

Development of a novel protocol for isolation and purification of human granulosa cells

“…Glycophorin A was chosen as the antibody to directly reduce RBCs because it is a known surface molecule on the RBC [31] and had been used by this lab before in other separation procedures. Noteably, this buffer was officially made to remove RBCs in mice, but its composition is quite similar to that for humans.…”

BLOOD COMPONENTS: A new one‐platform flow cytometric method for residual cell counting in platelet concentrates

“…The gating strategy is described in detail by Lambrecht and coworkers 25 . In brief, the Thrombo Count assay uses the PI nucleic acid stain, PE‐conjugated antibody, and Trucount beads, which all emit strong fluorescence above 580 nm and are detectable by the FL2 detector (Fig.…”