A New Filter Cassette for the Direct Microscopic Examination of Airborne Fungal Spores

Spurgeon, Joe C.

doi:10.1080/02786820600924960

Cited by 3 publications

(7 citation statements)

References 11 publications

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“…Table 1 The average Asp/Pen concentration in Table 1 that was detected with the BA in a well-mixed room was 2.1 times the average concentration detected with the AOC. This ratio was equivalent to that obtained in a previous study conducted in a well-mixed settling chamber (Spurgeon 2006). Therefore, the results for Asp/Pen spores collected with the BA and the AOC were consistent with those of the previous chamber study.…”

Section: Resultssupporting

confidence: 79%

“…Differences in spore retention were evaluated based on the concentrations of Asp/Pen and Chaetomium spores detected in replicate field samples. A second objective of this study was to compare the BA and the AOC at Asp/Pen concentrations higher than those used in the previous chamber study (Spurgeon 2006).…”

Section: Purposementioning

confidence: 99%

“…Third, their ability to retain spores is affected by particle size and spore morphology, resulting in variable retention rates for different spore types. In addition, the gap between the sampler inlet and the impaction surface results in an unbounded and diffuse sample trace (rectangular or circular area on the collection medium in which the sample has been deposited), increasing the difficulty of detecting all the deposited spores (Aizenberg et al 2000;Spurgeon 2006). …”

Section: Introductionmentioning

confidence: 99%

“…The Bi-Air (BA) filter cassette was specifically designed for the collection of airborne fungal spores, and has many of the desirable characteristics of a bioaerosol sampler (Spurgeon 2006). It also has a relatively small sample trace with well defined boundaries; a design that produces duplicate sample traces; and the ability to vary capture velocity independently of airflow rate.…”

Section: Introductionmentioning

confidence: 99%

“…A previous study compared the performance of the BA and the AOC for the collection of Asp/Pen spores under controlled conditions in a quiescent settling chamber (Spurgeon 2006). The settling chamber was representative of a well-mixed environment, whereas field investigations are typically performed under less controlled conditions.…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of Replicate Field Samples Collected with the Bi-Air, Air-O-Cell, and Graesby-Andersen N6 Bioaerosol Samplers

Spurgeon

2007

Aerosol Science and Technology

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The Bi-Air (BA) and Air-O-Cell (AOC) cassettes have been compared for the collection of Aspergillus/Penicillium (Asp/Pen) and Chaetomium spores based on replicate and/or duplicate samples collected under typical field conditions. Total culturable Asp and Pen concentrations were also compared for the BA and the N6 impactor based on duplicate field samples. When single Asp/Pen spores or small chains were dominant, the average BA:AOC concentration ratio was 2.1 (11,260 spores/m 3 v 5,400 spores/m 3 ) for six duplicate samples collected in a well-mixed room. This ratio was consistent with previous studies performed under controlled conditions. However, the average BA:AOC concentration ratio was 54 (765,00 spores/m 3 v 36,800 spores/m 3 ) for four replicate samples in which small Asp/Pen spores and clusters of Asp/Pen spores were dominant. The average BA:AOC concentration ratio for Chaetomium was typically 8.6, with a high of 100 for the four replicate samples in which Asp/Pen clusters were dominant.It was concluded that the performance of the BA and the AOC, except for a reasonably constant 2:1 ratio of concentrations, were similar for the detection of single Asp/Pen spores or small chains. However, the BA generally detected higher concentrations of Asp/Pen spores when clusters were dominant; and detected Chaetomium spores in more of the replicate field samples and at a higher average concentration.The average concentrations of culturable Aspergillus and Penicillium were not statistically different for six duplicate N6 and BA samples collected in a well-mixed room. For nine duplicate field samples with N6 concentrations of total culturable fungi greater than 1,000 cfu/m 3 , the N6 and BA were moderately correlated (r = 0.76). It was concluded that the ability of the BA to collect culturable fungi was similar to that of the N6 for short-term samples.

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Section: Resultssupporting

confidence: 79%

Section: Purposementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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