Bioaerosol sampling: Classical approaches, advances, and perspectives

Mainelis, Gediminas

doi:10.1080/02786826.2019.1671950

Cited by 139 publications

(127 citation statements)

References 195 publications

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“…Larger particles can generally be collected with higher efficiency than smaller particles (Morris, Leyronas, and Nicot 2014;Reponen et al 2001), though large particles (especially bacterial agglomerates, spores, and pollen) are frequently missed due to particle loss caused by poor sampling strategies. A well-designed and characterized inlet system can thus help minimize and quantify biases in the results (Mainelis 2019;Wiedensohler et al 2014;Von Der Weiden, Drewnick, and Borrmann 2009).…”

Section: Biogeography and Biodiversitymentioning

confidence: 99%

“…1. Standardization of collection strategies concerning the spatial and temporal resolution, replicated sampling, appropriate sterile practices and controls, improved intake efficiency, losses in ducts and tubes, increased sample biomass (i.e., high-volume bioaerosol sampling technique with high concentrate rate) and techniques allowing access to microbial in situ states (Cox et al 2019;Mainelis 2019). 2.…”

Section: Technical Challenges Recommendations and Needsmentioning

confidence: 99%

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Bioaerosol field measurements: Challenges and perspectives in outdoor studies

Šantl-Temkiv

Šikoparija

Maki

et al. 2019

Aerosol Science and Technology

105

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Outdoor field measurements of bioaerosols are performed within a wide range of basic and applied scientific disciplines, each with its own goals, assumptions, and terminology. This article contains brief reviews of outdoor field bioaerosol research from these diverse interests, with emphasis on perspectives from the atmospheric sciences. The focus is on a high-level discussion of pressing scientific questions, grand challenges, and needs for cross-disciplinary collaboration. The research topics, in which bioaerosol field measurement is important, include (i) atmospheric physics, clouds, climate, and hydrological cycle; (ii) atmospheric chemistry; (iii) airborne allergencontaining particles; (iv) airborne human pathogens and national security; (v) airborne livestock and crop pathogens; and (vi) biogeography and biodiversity. We concisely review bioaerosol impacts and discuss properties that distinguish bioaerosols from abiological aerosols. We give extra focus to regions of specific interest, i.e., forests, polar regions, marine and coastal environments, deserts, urban and rural areas, and summarize key considerations related to bioaerosol measurements, such as of fluxes, of long-range transport, and of sampling from both stationary and vessel-driven platforms. Keeping in mind a series of key scientific questions posed within the diverse communities, we suggest that pressing scientific questions include the following: (i) emission sources and flux estimates; (ii) spatial distribution; (iii) changes in distribution; (iv) atmospheric aging; (v) metabolic activity; (vi) urbanization of allergies; (vii) transport of human pathogens; and (viii) climate-relevant properties.

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Section: Biogeography and Biodiversitymentioning

confidence: 99%

Section: Technical Challenges Recommendations and Needsmentioning

confidence: 99%

Bioaerosol field measurements: Challenges and perspectives in outdoor studies

Šantl-Temkiv

Šikoparija

Maki

et al. 2019

Aerosol Science and Technology

105

View full text Add to dashboard Cite

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“…The vast majority of them are sufficiently small to enter and deposit within the human respiratory system. Therefore, it is vital to detect, identify and quantify bioaerosols, especially when considering their adverse effects on human health [1][2][3][4]. Besides, numerous studies have shown that bioaerosols play an important role in atmospheric processes [5][6][7], which provides an additional impetus for their sampling and identification of species or quantity.…”

Section: Introductionmentioning

confidence: 99%

“…Because bioaerosols are very diverse in terms of size, species and biological properties, so are the requirements for their detection. Numerous bioaerosol sampling methods [1] are available, including filtration, impaction, impingement and electrostatic precipitation [2,3]. Microscopy, flow cytometry, quantitative polymerase chain reaction, adenosine triphosphate (ATP)-based bioluminescence, culture-based techniques and other methods [4,5] can then be used to analyze the collected biological particles.…”

Section: Introductionmentioning

confidence: 99%

“…While these techniques have merit for their application in bioaerosol research, they have not been widely used in the bioaerosol field. Therefore, this study had the following objectives: (1) to compare the final volume and time needed to concentrate samples using the Evaporator; (2) to quantify particle losses determined by microscopy and culture-based analysis when using the two concentrating techniques in laboratory and outdoor field tests; and (3) determine the concentration factor (CF) of the devices based on the initial and final concentrations of the liquid-based particles.…”

Section: Introductionmentioning

confidence: 99%

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Performance of Two Different Techniques to Concentrate Samples for Bioaerosol Quantification

2020

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We evaluated two concentrating techniques that could be used to improve bioaerosol detection and quantification: A BioChromato Smart Evaporator C1 (BioChromato, Inc.) and two Concentrating Pipette (CP) models (CP-150 and CP-Select) (InnovaPrep, LLC). We determined the concentration factor (CF) (the concentration of particles in the final solution compared to the concentration in the initial solution) and the particle losses when processing the samples with polystyrene latex (PSL) beads and different species of bacteria. When processing total particles, regardless of the culturability status, the losses for the Evaporator were 3.70–23.89%; for the CP-models, the losses ranged from 0.20% to 67.22%. For the culturable particles processed with the CP devices, the losses ranged from 42.85% to 90.19% and were higher for Gram-negative pseudomonads compared to Gram-positive B. subtilis. Despite the loss of particles, both devices yielded more concentrated final solutions. The CF for the Evaporator was 3.59–10.92; the CF values for the CP devices ranged from 55.77 to 184.64 for total particles and from 6.29 to 96.52 for culturable bacteria. This higher CF was mainly achieved due to lower final suspension volumes. The study demonstrated that the two concentrators can improve particle detection, but that one should take particle losses into account.

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Airborne environmental DNA metabarcoding for the monitoring of terrestrial insects—A proof of concept from the field

et al. 2022

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Biodiversity is in decline due to human‐induced pressures on ecosystems around the world. To be able to counteract this alarming trend, it is paramount to closely monitor biodiversity at global scales. Because this is practically impossible with traditional methods, the last decade has seen a strong push for new solutions. In aquatic ecosystems, the monitoring of species from environmental DNA (eDNA) has emerged as one of the most powerful tools at our disposal, but in terrestrial ecosystems, the power of eDNA for monitoring has so far been hampered by the local scale of the samples. In this study, we report the successful detection of insects from airborne eDNA from samples taken in the field. We compare our results to two traditional insect monitoring methods (1) light traps for moth monitoring and (2) transect walks for the monitoring of butterflies and wild bees. Airborne eDNA metabarcoding revealed DNA from six classes of arthropods, and twelve order of insects—including representatives from the four largest orders: Diptera (flies), Lepidoptera (butterflies and moths), Coleoptera (beetles), and Hymenoptera (bees, wasps, and ants). We did not detect all species observed using traditional methods and suggest further directions for the development of airborne eDNA metabarcoding. We also recovered DNA from nine species of vertebrates, including frogs, birds, and mammals as well as from 12 other phyla. Airborne eDNA has the potential to become a powerful tool for terrestrial biodiversity monitoring, with many impactful applications including the monitoring of pests, invasive, or endangered species and disease vectors.

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Bioaerosol sampling: Classical approaches, advances, and perspectives

Cited by 139 publications

References 195 publications

Bioaerosol field measurements: Challenges and perspectives in outdoor studies

Bioaerosol field measurements: Challenges and perspectives in outdoor studies

Performance of Two Different Techniques to Concentrate Samples for Bioaerosol Quantification

Airborne environmental DNA metabarcoding for the monitoring of terrestrial insects—A proof of concept from the field

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