A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium.

Evans, M.C.W.; Buchanan, Bob B.; Arnon, Daniel I.

doi:10.1073/pnas.55.4.928

Cited by 493 publications

(251 citation statements)

References 18 publications

Supporting

Mentioning

242

Contrasting

Unclassified

Order By: Relevance

“…S2 in the supplemental material). The reductive citric acid cycle is generally considered the most energy-efficient CO 2 fixation pathway (ϳ0.6 mol ATP/mol CO 2 for pyruvate) (15,17,23,47). This is reflected by the activity of the pathway's key carboxylases, isocitrate dehydrogenase, ␣-ketoglutarate:ferredoxin oxidoreductase, and pyruvate: ferredoxin oxidoreductase.…”

Section: Carboxylases In Autotrophic Pathwaysmentioning

confidence: 99%

“…S1 to S4 in the supplemental material): (i) the reductive citric acid cycle (47), (ii) the reductive acetyl-CoA pathway (100), (iii) the 3-hydroxypropionate (or Fuchs-Holo) bicycle (61,134), (iv) the hydroxypropionate/hydroxybutytrate cycle (16), and (v) the dicarboxylate/hydroxybutyrate cycle (64). Like the CBB cycle, these "alternative" autotrophic pathways all use carboxylases as key enzymes for the fixation of CO 2 .…”

Section: Carboxylases In Autotrophic Pathwaysmentioning

confidence: 99%

See 1 more Smart Citation

Carboxylases in Natural and Synthetic Microbial Pathways

Erb

2011

Appl Environ Microbiol

170

159

View full text Add to dashboard Cite

Carboxylases are among the most important enzymes in the biosphere, because they catalyze a key reaction in the global carbon cycle: the fixation of inorganic carbon (CO 2 ). This minireview discusses the physiological roles of carboxylases in different microbial pathways that range from autotrophy, carbon assimilation, and anaplerosis to biosynthetic and redox-balancing functions. In addition, the current and possible future uses of carboxylation reactions in synthetic biology are discussed. Such uses include the possible transformation of the greenhouse gas carbon dioxide into value-added compounds and the production of novel antibiotics.

show abstract

Section: Carboxylases In Autotrophic Pathwaysmentioning

confidence: 99%

Section: Carboxylases In Autotrophic Pathwaysmentioning

confidence: 99%

Carboxylases in Natural and Synthetic Microbial Pathways

Erb

2011

Appl Environ Microbiol

170

159

View full text Add to dashboard Cite

show abstract

“…Abbreviations: Ru5P, ribulose-5-phosphate; RuBP, ribulose-l,5-bisphosphate; PGA, 3-phosphoglycerate; GAP, glyceraldehyde-3-phosphate; FBP, fructose-1,6-bisphosphate; F6P, ffuctose-6-phosphate; DtlAP, dihydroxyacetonephosphate; E4P, erythrose-4-phosphate; X5P, xylulose-5-phosphate; SBP, sedoheptulose-l,7-bisphosphate; S7P, sedoheptulose-7-phosphate; Ri5P, ribose-5-phosphate. Modified after Calvin (1962), are the Calvin cycle, found in the majority of autotrophic bacteria (Ohmann, 1979), the Reductive Carboxylic Acid cycle, in phototrophic bacteria belonging to the genus Chlorobium (Evans et al, 1966) and a linear pathway, encountered in non-phototrophic anaerobic bacteria (methanogens, acetogens and sulphatereducing bacteria). Via this latter pathway the formation of acetyl-CoA from two molecules of CO2 is accomplished (Zeikus, 1983;Fuchs and Stupperich, 1984).…”

Section: Introductionmentioning

confidence: 99%

Current views on the regulation of autotrophic carbon dioxide fixation via the Calvin cycle in bacteria

Dijkhuizen

Harder

1984

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

“…These are the reductive tricarboxylic acid (rTCA) cycle, postulated in the 1960s (14); the oxygen-sensitive reductive acetyl-CoA (rAcCoA) pathway (15); the extensively researched 3-hydroxypropionate cycle (16); the 3-hydroxypropionate/4-hydroxybutyrate cycle (17); and the recently discovered dicarboxylate/4-hydroxybutyrate cycle (18).…”

mentioning

confidence: 99%

Design and analysis of synthetic carbon fixation pathways

Bar‐Even¹,

Noor²,

Lewis

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

413

386

View full text Add to dashboard Cite

Carbon fixation is the process by which CO 2 is incorporated into organic compounds. In modern agriculture in which water, light, and nutrients can be abundant, carbon fixation could become a significant growth-limiting factor. Hence, increasing the fixation rate is of major importance in the road toward sustainability in food and energy production. There have been recent attempts to improve the rate and specificity of Rubisco, the carboxylating enzyme operating in the Calvin-Benson cycle; however, they have achieved only limited success. Nature employs several alternative carbon fixation pathways, which prompted us to ask whether more efficient novel synthetic cycles could be devised. Using the entire repertoire of approximately 5,000 metabolic enzymes known to occur in nature, we computationally identified alternative carbon fixation pathways that combine existing metabolic building blocks from various organisms. We compared the natural and synthetic pathways based on physicochemical criteria that include kinetics, energetics, and topology. Our study suggests that some of the proposed synthetic pathways could have significant quantitative advantages over their natural counterparts, such as the overall kinetic rate. One such cycle, which is predicted to be two to three times faster than the Calvin-Benson cycle, employs the most effective carboxylating enzyme, phosphoenolpyruvate carboxylase, using the core of the naturally evolved C4 cycle. Although implementing such alternative cycles presents daunting challenges related to expression levels, activity, stability, localization, and regulation, we believe our findings suggest exciting avenues of exploration in the grand challenge of enhancing food and renewable fuel production via metabolic engineering and synthetic biology. metabolic engineering | synthetic biology | photosynthesis | carboxylation | biological optimization I n the process of transforming sunlight into stored chemical energy, plants absorb approximately 10 times more CO 2 from the atmosphere than the total amount emitted by human activities globally (1). Moreover, agriculture, which is effectively a massive carbon fixation industry, makes use of the majority of cultivatable land on earth and accounts for most of the fresh water used by humanity (2). These figures point to the central role that carbon fixation by plants plays in our global ecological footprint. In nature, the factors limiting the growth of photosynthetic organisms vary among habitats and often include the availability of water, light, fixed nitrogen, iron, and phosphorus (e.g., ref.3). However, in agriculture today, the use of fertilizers and irrigation can make carbon fixation a rate-limiting factor. For example, many C3 plants have shown a significant increase in biomass when exposed to twice the atmospheric CO 2 concentration (4). Impressive growth enhancements have been demonstrated by addressing several biochemical limiting factors, related both to the light-dependent and light-independent reactions (5-9). For instance, tran...

show abstract

A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium.

Cited by 493 publications

References 18 publications

Carboxylases in Natural and Synthetic Microbial Pathways

Carboxylases in Natural and Synthetic Microbial Pathways

Current views on the regulation of autotrophic carbon dioxide fixation via the Calvin cycle in bacteria

Design and analysis of synthetic carbon fixation pathways

Contact Info

Product

Resources

About