A new extraction method of bioflavanoids from poisonous plant (Gratiola Officinalis L.)

Abstract

Abstract:The way of vegetable raw materials extraction which allows to receive nontoxical composition of biological active agents from poisonous plants such as Gratiola officinalis L. was described. The alkaloids exit changes with the increase of ethyl alcohol percentage (from 15% to 96%). The extract was obtained using 96% ethanol and did not give positive high quality reaction to the content of alkaloids. The chemical composition with new nontoxical biological active composition of Gratiola officinalis L. extract was investigated. The extract contains a previously unknown plant -bioflavonoid quercetin. The average value of quercetin in this extract using the calibration curve of the standard sample quercetin (98%) Sigma is 0.66%. In the dry rest of extractive substances (Gratiola officinalis L.) the quantity of quercetin was 350 mkg (obtained from 10 g of a dry grass) as was established by the method of a liquid chromatography.

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

“…Previously, we have developed a method for separation of flavonoid-containing extracts from Gratīola officinālis L. that allows obtaining non-toxic or low toxic extracts even from poisonous plants [5,6]. It is know that these extract have antioxidant [7,8], antitumor [9][10][11][12][13], antimicrobial and antiinflammatory [14,15] actions.…”

Effect of flavonoid-containing extracts on the growth of transplanted sarcoma 45, peripheral blood and bone marrow condition after oral and intramuscular administration in rats

“…In this work we used extracts which have been obtained by a process of double extraction, followed by alcohol precipitation of non-polar compounds (glycosides and alkaloids, et al) by chloroform from Gratiola officinalis plant, Helichrýsum arenárium and Zea mays [3][4][5]. This method has been patented by us.…”

“…Previously, we have developed a method for producing flavonoid-containing extracts from Gratīola officinālis, Helichrýsum arenárium, and Zea mays, that allows obtaining non-toxic or slightly toxic extracts even from poisonous plants [3][4][5][6]. It is know that these extracts have antioxidant, antitumor, immunomodulatory [7][8][9][10][11] and antimicrobial [12][13][14] actions.…”

Evaluation of the antitumor and anticachexia activity of gratiola officinalis L. Extract in rats with transplanted sarcoma 45

“…Материалом исследования послужил экс-тракт аврана лекарственного, полученного авторским запатентованным способом [11,20]. В него входят следующие химические ве-щества: 4-винил-2-метоксифенол; 2,3-дигидро-3 , 5 -д и г и д р о кс и -6 -м е т и л -4 Н -п и р а н -4 -о н ; 2,3-дигидробензофуран; 3-фуранкарбоновая кис-лота; 5-гидроксиметил-2-фуральдегид; этил-α-d-рибозид; 4-пропилфенол; пирокатехин; L-луксоза (пентоза); 6-деоксигексоза L-галактоза; бензоилук-сусной кислоты этиловый эфир; гексадекановая кислота (пальмитиновая кислота); гомованили-новая кислота; глюкоза; 1,4-ангидро-d-маннитол; бензойная кислота; кверцетин и ряд других флавоноидов.…”

“…Полученный автор-ским способом [12,20] экстракт не является ток-сичным [1,6] и, кроме того, способен благоприятно влиять и на состояние красного костного мозга [9]. Ранее антикахексический эффект экстракта аврана лекарственного не изучался.…”