Effect of flavonoid-containing extracts on the growth of transplanted sarcoma 45, peripheral blood and bone marrow condition after oral and intramuscular administration in rats

Navolokin, Nikita; Mudrak, D. A.; Alla, B Bucharskaya; Olga, V Matveeva; Sergey, A Tychina; Natalya, V Polukonova; Maslyakova, Galina N.

doi:10.15275/rusomj.2017.0304

Cited by 13 publications

(11 citation statements)

References 14 publications

(17 reference statements)

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“…Ранее на лабораторных животных с перевиваемыми опухолями было показано, что флавоноидсодержащий экстракт аврана лекарственного (Gratiola officinalis L.) наряду с малой токсичностью обладает противоопухолевой активностью в отношении перевиваемого рака печени и саркомы, а также антиоксидантным и антикахексическим свойством [5][6][7]. Однако детального изучения апоптоза, аутофагии и механизмов гибели опухолевых клеток перевиваемого рака почки с применением иммуногистохимических маркеров под действием экстракта аврана ранее не проводилось.…”

Section: Original Investigationsunclassified

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

et al. 2019

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Цель исследования-в экспериментах in vivo установить механизмы патоморфоза перевитого рака почки под влиянием флавоноидсодержащего экстракта аврана. Материал и методы. Эксперимент проводили на 30 самцах крыс Wistar c перевитым раком почки РА. В опытных группах крысы через 72 ч после перевивки опухоли на протяжении 12 сут получали экстракт аврана перорально или внутримышечно в дозе 110 мг/кг/сут. Группу сравнения составили животные с опухолью, но без воздействия. Использовали иммуногистохимические маркеры апоптоза (p53, bax, bcl-2, Fas-receptor, Fas-ligand), аутофагии LС3b, пролиферации Ki-67 и ангиогенеза VEGF. При статистической обработке данных нормальность распределения показателей в группах проверяли при помощи критерия Шапиро-Уилка. Для сравнения групп использовали критерий Крамера-Уэлча (Т). Результаты. При гистологическом исследовании в ткани опухоли под действием экстракта аврана наблюдали появление обширных зон повреждения (некроз и апоптозные тельца). При обоих путях введения экстракта аврана отмечали резкое снижение экспрессии маркеров пролиферации (Ki-67), ангиогенеза (VEGF) и высокую экспрессию маркеров апоптоза (p53, bax, CD95 (Fas/APO-1), FAS-ligand) в опухолевых клетках и отсутствие ее в группе сравнения. Все описанные изменения были более выражены при внутримышечном введении. Экспрессия маркера аутофагии LC3B увеличивалась при пероральном и уменьшалась при внутримышечном введении экстракта аврана. Заключение. Под влиянием экстракта аврана развивается выраженный патоморфоз рака почки. Происходят подавление пролиферации, ангиогенеза и активация сигнального и митохондриального пути апоптоза, а также блокирование протекторной аутофагии. Маркер аутофагии LC3b может использоваться в качестве дополнительного критерия оценки лечебного патоморфоза опухолей.

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Section: Original Investigationsunclassified

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

et al. 2019

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“…Previously, we have developed a method for separation of flavonoid-containing extracts from Gratīola officinālis L. that allows obtaining non-toxic or low toxic extracts even from poisonous plants [5,6]. It is know that these extract have antioxidant [7,8], antitumor [9][10][11][12][13], antimicrobial and antiinflammatory [14,15] actions.…”

Section: Introductionmentioning

confidence: 99%

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Polukonova¹,

Navolokin²,

Bucharskaya³

et al. 2018

Russ Open Med J

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Objective -The discovery of the apoptosis-inducing effects of flavonoid vagonin allowed to make an assumption of existence of similar effect in others flavonoids. This study is devoted to the effects of Gratīola officinālis extract on cell culture of the human kidney cancer.Methods -Cell cultures of human kidney carcinoma -Caki-1 and SN12c were used in the study. The cells were stained with Hoechat 33258 dye. The number of living cells, cells in a state of apoptosis and mitosis were counted. The Cramer-Welch criterion (T) was used to compare the obtained data.Results -The activation of apoptosis was noted at all concentrations of the Gratiola officinalis L. extract during the first day of exposure. The apoptotic activity increased with increasing of extract concentration. After 48 hours, this activity was maintained only at a Gratiola officinalis L. extract concentration of 0.9 mg/ml. After 24 hours, the apoptotic activity of the extract was more expressed in the culture of CaKi-1. However, after 48 hours the extract induces more pronounced apoptosis in the culture of Sn12c cells. The cytotoxic activity of the extract was not differ after 24 hours in both cultures, after 48 hours it was more pronounced in the culture of CaKi-1. Conclusion -We revealed a pronounced antitumor and apoptotic activity of the Gratiola officinalis L. extract against the cultures of the kidney cancer Caki-1 and Sn12c. Apoptosis of tumor cells can be manifested in the form of pycnosis of the nucleus, the formation of apoptotic bodies and the emergence of cellular debris resulting from complete degradation of tumor cells. The apoptotic activity of the Gratiola officinalis extract depends on their concentration.

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“…Herein, we present only 4 of them. Histological sections were stained with standard haematoxylin and eosin dyes [21].…”

Section: Effective Permittivity Determination Based On Metamaterials Fmentioning

confidence: 99%

Metamaterial formalism approach for advancing the recognition of glioma areas in brain tissue biopsies

Gric¹,

Sokolovski²,

Navolokin³

et al. 2020

Opt. Mater. Express

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Early detection of a tumor makes it more probable that the patient will, finally, beat cancer and recover. The main goal of broadly defined cancer diagnostics is to determine whether a patient has a tumor, where it is located, and its histological type and severity. The major characteristic of the cancer affected tissue is the presence of the glioma cells in the sample. The current approach in diagnosis focuses mainly on microbiological, immunological, and pathological aspects rather than on the "metamaterial geometry" of the diseases. The determination of the effective properties of the biological tissue samples and treating them as disordered metamaterial media has become possible with the development of effective medium approximation techniques. Their advantage lies in their capability to treat the biological tissue samples as metamaterial structures, possessing the well-studied properties. Here, we present, for the first time to our knowledge, the studies on metamaterial properties of biological tissues to identify healthy and cancerous areas in the brain tissue. The results show that the metamaterial properties strongly differ depending on the tissue type, if it is healthy or unhealthy. The obtained effective permittivity values were dependent on various factors, like the amount of different cell types in the sample and their distribution. Based on these findings, the identification of the cancer affected areas based on their effective medium properties was performed. These results prove the metamaterial model capability in recognition of the cancer affected areas. The presented approach can have a significant impact on the development of methodological approaches toward precise identification of pathological tissues and would allow for more effective detection of cancer-related changes.

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Effect of flavonoid-containing extracts on the growth of transplanted sarcoma 45, peripheral blood and bone marrow condition after oral and intramuscular administration in rats

Cited by 13 publications

References 14 publications

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Metamaterial formalism approach for advancing the recognition of glioma areas in brain tissue biopsies

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