A new endornavirus species infecting Malabar spinach (Basella alba L.)

Abstract

A putative new endornavirus was isolated from Malabar spinach (Basella alba). The viral dsRNA consisted of 14,027 nt with a single ORF that coded for a polyprotein of 4,508 aa. The genome organization was similar to that of four other endornaviruses. Conserved domains for helicase-1, capsular synthase, UDP-glucose-glycosyltransferase (UGT), and RdRp were detected. Infected plants were phenotypically undistinguishable from healthy ones. The name Basella alba endornavirus is proposed for the virus isolated from Malabar spinach.

Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

“…Currently, all plant-infecting endornaviruses have been assigned to the genus Alphaendornavirus by 10th Report of ICTV. They infect several economically important crops such as avocado, common bean, spinach, bottle gourd, pepper and rice (Moriyama et al 1999;Okada et al 2011;Villanueva et al 2012;Okada et al 2013;Kwon et al 2014;Okada et al 2014;Lim et al 2015). These dsRNA viruses occur at every developmental stages and tissues of host, and replicate in the host cells (Moriyama et al 1995).…”

Cucumis melo endornavirus: Genome organization, host range and co-divergence with the host

“…Genomes of the following recognized or putative endornaviruses have been completely sequenced and characterized (in chronological order): Oryza sativa endornavirus (OsEV, Moriyama et al, 1995), Vicia faba endornavirus (Pfeiffer, 1998), Oryza rufipogon endornavirus (OrEV; Moriyama et al, 1999), Phytophthora endornavirus 1 (PEV-1; Hacker et al, 2005), Helicobasidium mompa endornavirus 1 (Osaki et al, 2006), Gremmeniella abietina type B RNA virus XL (GaBRV-XL; Tuomivirta et al, 2009), Tuber aestivum endornavirus (TaEV; Stielow et al, 2011), bell pepper endornavirus (BPEV; Okada et al, 2011;Sela et al, 2012), Persea americana endornavirus (PaEV;Villanueva et al, 2012), Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2 (PvEV-1 and PvEV-2; Okada et al, 2013), grapevine endophyte endornavirus (GeEV; Espach et al, 2012), Basella alba endornavirus (BaEV; Okada et al, 2014), Lagenaria siceraria endornavirus (LsEV) (Kwon et al, 2014), Yerba mate endornavirus (Debat et al, 2014), Rhizoctonia cerealis endornavirus 1 (RcEV1; Li et al, 2014), Rhizoctonia solani endornavirus -RS002 (RsEV; Das et al, 2014), Sclerotinia sclerotiorum endornavirus 1 (SsEV1; Khalifa and Pearson, 2014), Thielaviopsis basicola endornavirus (Chen and Punja, 2014), and Alternaria brassicola endornavirus (Shang et al, 2015). In addition, several partially characterized endornaviruses have been reported from seagrass (Zostera marina), barley (Hordeum vulgare), and melon (Cucumis melo) (Coutts, 2005;Fukuhara et al, 2006;Quito-Avila et al, 2014).…”

A simple and rapid method to purify viral dsRNA from plant and fungal tissue

“…2a (Moriyama et al 1995;Okada et al 2011Okada et al , 2013Okada et al , 2014. Furthermore, screening of winged bean resulted in the detection of an approximately 15 kbp endornavirus-like dsRNA (Fig.…”