A new double emulsion solvent diffusion technique for encapsulating hydrophilic molecules in PLGA nanoparticles

Cohen-Sela, Einat; Chorny, Michael; Koroukhov, Nickolay; Danenberg, Haim; Golomb, Gershon

doi:10.1016/j.jconrel.2008.09.073

Cited by 256 publications

(182 citation statements)

References 28 publications

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“…As a model drug, alendronate was used in in vitro studies, and the inhibitory effect of the particle-associated bisphosphonate on osteoclasts (and their precursors) was confirmed for nondegradable gold nanoparticles as well as for biodegradable nanoparticles. 46,54 Alendronate-modified nanoparticles were localized in the cytosol and, as expected, were observed to reduce the number of multinucleated, tartrate-resistant acid phosphatasepositive cells. 46 However, based on the inhomogeneity of the osteoclast cell culture due to the differing stages of osteoclast development at a given point in time, the effects submit your manuscript | www.dovepress.com of encapsulated, osteoclast-influencing substances are diff icult to identify.…”

Section: Drug Deliverysupporting

confidence: 66%

“…9,10 The following paragraph focuses on the use of nanoparticles as an effective protein or drug delivery system to support bone tissue regeneration. For this purpose, several nondegradable particles, such as silica, lipid, dendrimer, hydroxyapatite, or gold nanoparticles, [43][44][45][46][47][48][49][50] as well as degradable particles made of poly(L-lactide) 51,52 or poly(L-lactideco-glycolide) (PLGA), 11,42,[52][53][54][55][56] have been used. Frequently, nanoparticles were combined with scaffolds such as proteinaceous hydrogels or degradable polymeric matrixes to facilitate application in bone.…”

Section: Drug Deliverymentioning

confidence: 99%

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Nanoparticles and their potential for application in bone

Tautzenberger¹,

Kovtun²,

Ignatius³

2012

IJN

161

137

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Abstract:Biomaterials are commonly applied in regenerative therapy and tissue engineering in bone, and have been substantially refined in recent years. Thereby, research approaches focus more and more on nanoparticles, which have great potential for a variety of applications. Generally, nanoparticles interact distinctively with bone cells and tissue, depending on their composition, size, and shape. Therefore, detailed analyses of nanoparticle effects on cellular functions have been performed to select the most suitable candidates for supporting bone regeneration. This review will highlight potential nanoparticle applications in bone, focusing on cell labeling as well as drug and gene delivery. Labeling, eg, of mesenchymal stem cells, which display exceptional regenerative potential, makes monitoring and evaluation of cell therapy approaches possible. By including bioactive molecules in nanoparticles, locally and temporally controlled support of tissue regeneration is feasible, eg, to directly influence osteoblast differentiation or excessive osteoclast behavior. In addition, the delivery of genetic material with nanoparticulate carriers offers the possibility of overcoming certain disadvantages of standard protein delivery approaches, such as aggregation in the bloodstream during systemic therapy. Moreover, nanoparticles are already clinically applied in cancer treatment. Thus, corresponding efforts could lead to new therapeutic strategies to improve bone regeneration or to treat bone disorders.

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Section: Drug Deliverysupporting

confidence: 66%

Section: Drug Deliverymentioning

confidence: 99%

Nanoparticles and their potential for application in bone

Tautzenberger¹,

Kovtun²,

Ignatius³

2012

IJN

161

137

View full text Add to dashboard Cite

show abstract

“…PLGA nanoparticles were prepared using a modified double emulsion solvent diffusion method (Cohen-Sela et al, 2009). The procedure in brief is as follows.…”

Section: Preparation Of Bsa Loaded P L G a Nanoparticlesmentioning

confidence: 99%

Incorporation of PLGA nanoparticles into porous chitosan–gelatin scaffolds: Influence on the physical properties and cell behavior

Nandagiri

Gentile

Chiono

et al. 2011

Journal of the Mechanical Behavior of Biomedical Materials

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“…Very large particles (around 1 μm) can cause irritant effects after injection. 34 On the other hand, particles smaller than 5 nm are likely to be cleared by non-phagocytic cells. 35 Alternatively, particles larger than 100 nm have the chance to be taken up by phagocytic cells (for example, DCs).…”

Section: Discussionmentioning

confidence: 99%

Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles

Jahan¹,

Haddadi²

2015

IJN

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The purpose of this study was to assess the effect of various formulation parameters on anti-CD205 antibody decorated poly( d , l -lactide co-glycolide) (PLGA) nanoparticles (NPs) in terms of their ability to target dendritic cells (DCs). In brief, emulsification solvent evaporation technique was adapted to design NP formulations using two different viscosity grades (low and high) of both ester and carboxylic acid terminated PLGA. Incorporation of ligand was achieved following physical adsorption or chemical conjugation processes. The physicochemical characterizations of formulations were executed to assess the effects of different solvents (chloroform and ethyl acetate), stabilizer percentage, polymer types, polymer viscosities, ligand-NP bonding types, cross-linkers, and cryoprotectants (sucrose and trehalose). Modification of any of these parameters shows significant improvement of physicochemical properties of NPs. Ethyl acetate was the solvent of choice for the formulations to ensure better emulsion formation. Infrared spectroscopy confirmed the presence of anti-CD205 antibody in the NP formulation. Finally, cytotoxicity assay confirmed the safety profile of the NPs for DCs. Thus, ligand modified structurally concealed PLGA NPs is a promising delivery tool for targeting DCs in vivo.

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A new double emulsion solvent diffusion technique for encapsulating hydrophilic molecules in PLGA nanoparticles

Cited by 256 publications

References 28 publications

Nanoparticles and their potential for application in bone

Nanoparticles and their potential for application in bone

Incorporation of PLGA nanoparticles into porous chitosan–gelatin scaffolds: Influence on the physical properties and cell behavior

Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles

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