A new donor-acceptor conjugated polymer-gold nanoparticles biocomposite materials for enzymatic determination of glucose

Tan, Burcu; Baycan, Fatma

doi:10.1016/j.polymer.2020.123066

Cited by 13 publications

(8 citation statements)

References 36 publications

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“…The stabilizing agents may also have specific new functions, such as detection, therapy, or vectorization. Potential agents include a wide variety of biomolecules, such as peptides [ 32 ], proteins [ 33 , 34 ], genetic material [ 35 , 36 ], antibodies [ 37 , 38 ], drugs [ 39 , 40 ], Raman reporters [ 41 , 42 ], fluorophores [ 26 , 43 ], polymers [ 44 , 45 , 46 ], and supramolecular complexes [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

et al. 2021

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Gold nanostars (AuNSs) exhibit modulated plasmon resonance and have a high SERS enhancement factor. However, their low colloidal stability limits their biomedical application as a nanomaterial. Cationic β-cyclodextrin-based polymer (CCD/P) has low cytotoxicity, can load and transport drugs more efficiently than the corresponding monomeric form, and has an appropriate cationic group to stabilize gold nanoparticles. In this work, we functionalized AuNSs with CCD/P to load phenylethylamine (PhEA) and piperine (PIP) and evaluated SERS-based applications of the products. PhEA and PIP were included in the polymer and used to functionalize AuNSs, forming a new AuNS-CCD/P-PhEA-PIP nanosystem. The system was characterized by UV–VIS, IR, and NMR spectroscopy, TGA, SPR, DLS, zeta potential analysis, FE-SEM, and TEM. Additionally, Raman optical activity, SERS analysis and complementary theoretical studies were used for characterization. Minor adjustments increased the colloidal stability of AuNSs. The loading capacity of the CCD/P with PhEA-PIP was 95 ± 7%. The physicochemical parameters of the AuNS-CCD/P-PhEA-PIP system, such as size and Z potential, are suitable for potential biomedical applications Raman and SERS studies were used to monitor PhEA and PIP loading and their preferential orientation upon interaction with the surface of AuNSs. This unique nanomaterial could be used for simultaneous drug loading and SERS-based detection.

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Section: Introductionmentioning

confidence: 99%

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

et al. 2021

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“…The cross-linking method was used for GOx immobilization on the PHCQE/AuNPs electrode [ 62 ]. Firstly, the HCQE (3-(5,8-bis (2,3-dihydrothieno[3,4-b][ 1 , 4 ]dioxin-5-yl)-3-(9-hexyl-9H-carbazole-3-yl) quinoxalin-2-yl)-9-hexyl9H-carbazole) was electropolymerized on to a GPE (glass pencil electrode).…”

Section: Types Of Immobilization Methodsmentioning

confidence: 99%

“… ( a ) Schematic representation of the preparation of PHCQE/AuNPs/GOx;( b ) SEM image of PHCQE/AuNPs; ( c ) SEM image of PHCQE/AuNPs/GOx; ( d ) CV curves for PHCQE/AuNPs and PHCQE/AuNPs/GOx electrodes in 0.1 M PBS without and with glucose. Reprinted with permission from [ 62 ]; Copyright 2020, Elsevier. …”

Section: Figurementioning

confidence: 99%

Enzyme Immobilization on Gold Nanoparticles for Electrochemical Glucose Biosensors

2021

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More than 50 years have passed since Clark and Lyon developed the concept of glucose biosensors. Extensive research about biosensors has been carried out up to this day, and an exponential trend in this topic can be observed. The scope of this review is to present various enzyme immobilization methods on gold nanoparticles used for glucose sensing over the past five years. This work covers covalent bonding, adsorption, cross-linking, entrapment, and self-assembled monolayer methods. The experimental approach of each modification as well as further results are described. Designated values of sensitivity, the limit of detection, and linear range are used for the comparison of immobilization techniques.

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“…Enzymatic glucose biosensors are generally based on two oxidoreductases: Glucose oxidase (GOx) [6–9] and Glucose dehydrogenase (GDH) [10–12] . Although electrochemical glucose biosensors based on these two enzyme subclasses have been studied extensively, the performance of enzyme‐based biosensors decreases due to the unstable nature of enzymes, low storage stability, expensiveness, and the effect of environmental conditions on enzymatic activity [13–15] . The most efficient immobilization of the enzyme on the electrode surface improves the sensing performance of the biosensor [5] .…”

Section: Introductionmentioning

confidence: 99%

“…For example, Tan et al. reported the monomer 3‐(5,8‐bis(2,3‐dihydrothieno[3,4‐b][1,4]dioxin‐5‐yl)‐3‐(9‐hexyl‐9H‐carbazol‐3‐yl) (HCQE) was electropolymerized with Au nanoparticles on the PGE as a glucose biosensor for the first time [15] . In a study by G. Gnana kumar et al., a free‐standing and flexible carbon nanofiber membrane (CNF) composed of carbon fibers decorated with NiMoO4 nanoparticles was developed for glucose sensing analysis.…”

Section: Introductionmentioning

confidence: 99%

An Enzymatic Glucose Biosensor Based on a Pencil Graphite Electrode Modified with Naphthalenedimide/3,4‐Ethylenedioxythiophene Conjugated Polymer and Enriched with Au Nanoparticles

Tan

Baycan

2022

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N,N′‐bis(2‐hexyl)‐2,6‐(3,4 ethylenedioxythiophene)‐1,4,5,8‐naphthalenimide (ENDI) monomer was electropolymerized on pencil graphite electrode (PGE) and used as a biosensor for glucose immobilization for the first time. After electropolymerization of ENDI monomer on PGE (AuNPs/PENDI/PGE), the intensity of the current signal was improved by using gold nanoparticles (AuNPs). The immobilization of the glucose oxidase (GOx) enzyme was carried out by using the cross‐linking reagent glutaraldehyde (GA) due to the strong π‐π* interaction of the poly‐ N,N′‐bis(2‐hexyl)‐2,6‐(3,4 ethylenedioxythiophene)‐1,4,5,8‐naphthalenimide (PENDI) polymer. GOx/AuNPs/PENDI/PGE designed biosensor, was characterized by a wide linear glucose determination range between 0.0009–0.33 mM with a limit of detection of 0.0407 mM and a sensitivity of 0.172 μA/mMcm2. Herein we have shown that GOx/AuNPs/PENDI/PGE biosensor effectively provides the enzyme immobilization and very effective for glucose determination in commercial samples.

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A new donor-acceptor conjugated polymer-gold nanoparticles biocomposite materials for enzymatic determination of glucose

Cited by 13 publications

References 36 publications

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

Enzyme Immobilization on Gold Nanoparticles for Electrochemical Glucose Biosensors

An Enzymatic Glucose Biosensor Based on a Pencil Graphite Electrode Modified with Naphthalenedimide/3,4‐Ethylenedioxythiophene Conjugated Polymer and Enriched with Au Nanoparticles

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