Enzyme Immobilization on Gold Nanoparticles for Electrochemical Glucose Biosensors

Lipińska, Wiktoria; Grochowska, Katarzyna; Siuzdak, Katarzyna

doi:10.3390/nano11051156

Cited by 28 publications

(15 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In conclusion, the MPA adsorption at the electrode surface is possible through both thiol and carboxyl species, while the Upon analyte biomolecule adsorption to the functionalized electrode surface, the electrochemically active surface decreases, hindering the redox process, which is easily observed by an R CT increase. 36,37 R CT was then used to estimate sensor performance Ψ, see SI, Section 3 for details. Most notably, the sensor performance Ψ strongly depends on the MPA concentration, indicating the key importance of this first functionalization step on the biosensor efficacy.…”

Section: Topography and Functionalization Efficacymentioning

confidence: 99%

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

et al. 2023

Self Cite

View full text Add to dashboard Cite

In this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au−Ti surface have preferential and stronger binding patterns through the carboxyl bond inducing an enhanced surface coverage with CD147. Control of bonding at the surface is essential for oriented receptor assembling and boosted sensitivity. The complex Au−Ti electrode texture along with optimized MPA concentration is a crucial parameter, enabling to reach the detection limit of ca. 3 ng mL −1 . Scanning electrochemical microscopy imaging and quantum molecular modeling were performed to understand the electrochemical performance and specific assembly of MPA displaying a free stereo orientation and not disturbed by direct interactions with closely adjacent receptors. This significantly limits nonspecific interceptor reactions, strongly decreasing the detection of receptor-binding domain proteins by saturation of binding groups. This method has been demonstrated for detecting the SARS virus but can generally be applied to a variety of protein−antigen systems. Moreover, the raster of the pattern can be tuned using various anodizing processes at the titania surfaces.

show abstract

Section: Topography and Functionalization Efficacymentioning

confidence: 99%

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the enzymatic glucose biosensors, the most important point is the immobilization with high stability of the enzyme on the appropriate substrate ( Lipińska et al, 2021a ). However, these biosensors lack long-term stability because of the properties of the enzyme.…”

Section: Background Knowledge For Glucose Sensorsmentioning

confidence: 99%

Electrospun nanofiber-based glucose sensors for glucose detection

Zhang

Liu

et al. 2022

Front. Chem.

View full text Add to dashboard Cite

Diabetes is a chronic, systemic metabolic disease that leads to multiple complications, even death. Meanwhile, the number of people with diabetes worldwide is increasing year by year. Sensors play an important role in the development of biomedical devices. The development of efficient, stable, and inexpensive glucose sensors for the continuous monitoring of blood glucose levels has received widespread attention because they can provide reliable data for diabetes prevention and diagnosis. Electrospun nanofibers are new kinds of functional nanocomposites that show incredible capabilities for high-level biosensing. This article reviews glucose sensors based on electrospun nanofibers. The principles of the glucose sensor, the types of glucose measurement, and the glucose detection methods are briefly discussed. The principle of electrospinning and its applications and advantages in glucose sensors are then introduced. This article provides a comprehensive summary of the applications and advantages of polymers and nanomaterials in electrospun nanofiber-based glucose sensors. The relevant applications and comparisons of enzymatic and non-enzymatic nanofiber-based glucose sensors are discussed in detail. The main advantages and disadvantages of glucose sensors based on electrospun nanofibers are evaluated, and some solutions are proposed. Finally, potential commercial development and improved methods for glucose sensors based on electrospinning nanofibers are discussed.

show abstract

“…Enzymes have a unique catalytic activity and specificity towards its target substrate, which results in the wide usage of the biomedical application. The immobilization of enzymes has received great attention due to their multiple or repeatable use for a particular purpose and improving their stability [ 28 ]. However, the high production costs and expensive separation techniques sometimes deter their usages.…”

Section: Gold Nanoparticle Based Biosensorsmentioning

confidence: 99%

Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry

Kulabhusan

Tripathi

Kant

2022

Sensors

View full text Add to dashboard Cite

Plant diseases and their diagnoses are currently one of the global challenges and causes significant impact to the economy of farmers and industries depending on plant-based products. Plant pathogens such as viruses, bacteria, fungi, and pollution caused by the nanomaterial, as well other important elements of pollution, are the main reason for the loss of plants in agriculture and in forest ecosystems. Presently, various techniques are used to detect pathogens in trees, which includes DNA-based techniques, as well as other microscopy based identification and detection. However, these methodologies require complex instruments and time. Lately, nanomaterial-based new biosensing systems for early detection of diseases, with specificity and sensitivity, are developed and applied. This review highlights the nanomaterial-based biosensing methods of disease detection. Precise and time effective identification of plant pathogens will help to reduce losses in agriculture and forestry. This review focuses on various plant diseases and the requirements for a reliable, fast, and cost-effective testing method, as well as new biosensing technologies for the detection of diseases of field plants in forests at early stages of their growth.

show abstract

Enzyme Immobilization on Gold Nanoparticles for Electrochemical Glucose Biosensors

Cited by 28 publications

References 76 publications

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

Electrospun nanofiber-based glucose sensors for glucose detection

Gold Nanoparticles and Plant Pathogens: An Overview and Prospective for Biosensing in Forestry

Contact Info

Product

Resources

About