A new direct-vision biopsy gastroscope

Debray, C; Housset, P; Martín, Elena Gallardo; Bourdais, Julien; Nicolaidis, Christina

doi:10.1136/gut.3.3.273

Cited by 8 publications

(2 citation statements)

References 9 publications

(7 reference statements)

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“…We used nuclear extracts from stomach mucosa obtained by gastric endoscope to test clinical sample applications . In this case, we used a piece of gastric endoscope sample weighing approximately 7–8 mg and detected 140 TFs (Figure b and Supplementary Table 6).…”

Section: Resultsmentioning

confidence: 99%

“…We used nuclear extracts from stomach mucosa obtained by gastric endoscope to test clinical sample applications. 13 In this case, we used a piece of gastric endoscope sample weighing approximately 7−8 mg and detected 140 TFs (Figure 4b and Supplementary Table 6). The whole process, from fresh biopsy sample collection to quantitative TF data production, took as little as 4 h. These results demonstrated that TOT is a practical approach to detect active endogenous TFs, which can be used not only in laboratory research but drug screening and clinical diagnosis.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Transcription Factor Response Elements on Tip: A Sensitive Approach for Large-Scale Endogenous Transcription Factor Quantitative Identification

Shi

Song

et al. 2016

Anal. Chem.

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The ability to map endogenous transcription factors (TFs) DNA binding activity at the proteome scale will greatly enhance our understanding of various biological processes. Here we report a highly sensitive, rapid, and high-throughput approach, transcription factor response elements on tip-mass spectrometry (TOT-MS), that allows for quantitative measurement of endogenous TFs. A total of 150 TFs from 1 μg of nuclear extracts can be quantified with single shot mass spectrometry detection in 1 h of machine time. Up to 755 TFs, which is comparable to the depth of RNA-seq, were identified by TOT coupled with on-tip small size reverse-phase liquid chromatography. We further demonstrated the capability of TOT-MS by interrogating the dynamic change of TFs in the epidermal growth factor (EGF) signaling pathway. This approach should find broad applications in elucidating the TF landscape from limited amounts of biological materials.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%