A new dilution-enrichment sample preparation strategy for expanded metabolome monitoring of human breast milk that overcomes the simultaneous presence of low- and high-abundance lipid species

Hewelt-Belka, Weronika; Garwolińska, Dorota; Belka, Mariusz; Bączek, Tomasz; Namieśnik, Jacek; Kot‐Wasik, Agata

doi:10.1016/j.foodchem.2019.03.001

Cited by 23 publications

(29 citation statements)

References 35 publications

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“…Our results show qualitative and quantitative differences between HBM and FM composition as well as within the FM of various brands and in HBM itself, which is consistent with other studies [15,16,[29][30][31]. Metabolite profiles of FM samples were distinct between studied brands.…”

Section: Discussionsupporting

confidence: 92%

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Nuclear Magnetic Resonance Metabolomics Reveals Qualitative and Quantitative Differences in the Composition of Human Breast Milk and Milk Formulas

et al. 2020

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Commercial formula milk (FM) constitutes the best alternative to fulfill the nutritional requirements of infants when breastfeeding is precluded. Here, we present the comparative study of polar metabolite composition of human breast milk (HBM) and seven different brands of FM by nuclear magnetic resonance spectroscopy. The results of the multivariate data analysis exposed qualitative and quantitative differences between HBM and FM composition as well as within FM of various brands and in HBM itself (between individual mothers and lactation period). Several metabolites were found exclusively in HBM and FM. Statistically significant higher levels of isoleucine and methionine in their free form were detected in FM samples based on caprine milk, while FM samples based on bovine milk showed a higher level of glucose and galactose in comparison to HBM. The results suggest that the amelioration of FM formulation is imperative to better mimic the composition of minor nutrients in HBM.

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Section: Discussionsupporting

confidence: 92%

“…Extraction of polar metabolites was based on previously published modified Bligh and Dyer extraction with small modifications [15]. A 225 µL HBM sample was briefly transferred to an Eppendorf microcentrifuge tube and mixed with 950 µL of chloroform/methanol mixture (1/2, v/v).…”

Section: Sample Preparationmentioning

confidence: 99%

Nuclear Magnetic Resonance Metabolomics Reveals Qualitative and Quantitative Differences in the Composition of Human Breast Milk and Milk Formulas

et al. 2020

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“…Sample preparation was performed using a previously developed extraction method based on the LLE and SPE techniques [ 30 ]. The HM and FM samples were extracted in duplicate.…”

Section: Methodsmentioning

confidence: 99%

“…Lipidomics is now widely used in the comprehensive analysis of the lipidomes of various biological samples and is a valuable tool to analyse human milk [ 12 , 25 , 26 , 27 , 28 , 29 , 30 ]. Although, only a few studies have investigated lipidomic differences between HM and FM [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Lipidomic Study of Human Milk from Different Lactation Stages and Milk Formulas

et al. 2020

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In this report, we present a detailed comparison of the lipid composition of human milk (HM) and formula milk (FM) targeting different lactation stages and infant age range. We studied HM samples collected from 26 Polish mothers from colostrum to 19 months of lactation, along with FM from seven brands available on the Polish market (infant formula, follow-on formula and growing-up formula). Lipid extracts were analysed using liquid chromatography coupled to high-resolution mass spectrometry (LC–Q-TOF–MS). We found that the lipid composition of FM deviates significantly from the HM lipid profile in terms of qualitative and quantitative differences. FM had contrasting lipid profiles mostly across brands and accordingly to the type of fat added but not specific to the target age range. The individual differences were dominant in HM; however, differences according to the lactation stage were also observed, especially between colostrum and HM collected in other lactation stages. Biologically and nutritionally important lipids, such as long-chain polyunsaturated fatty acids (LC-PUFAs) containing lipid species, sphingomyelines or ether analogues of glycerophosphoethanoloamines were detected in HM collected in all studied lactation stages. The observed differences concerned all the major HM lipid classes and highlight the importance of the detailed compositional studies of both HM and FM.

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“…SPE as an additional clean‐up step is usually used after LLE to enrich specific classes of targeted lipids, which may provide improved specificity and extraction efficiency for targeted lipidomics (Reyes‐Garcés & Gionfriddo, 2019). In the analysis of human breast milk (HBM), the high‐abundance lipid species, such as triglycerides (TAG) and diacylglycerides (DAG), were extracted through modified Bligh and Dyer method and the low‐abundance lipids species (PL, GP, and SP) were specifically enriched by SPE with a zirconia‐based stationary phase (Hewelt‐Belka et al., 2019). Simultaneous detection of both low‐ and high‐abundance HBM lipids can be achieved by coupling LLE and SPE techniques.…”

Section: The Analysis Workflow Of Ms‐based Lipidomicsmentioning

confidence: 99%

Mass spectrometry‐based lipidomics in food science and nutritional health: A comprehensive review

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Wang

Cong

et al. 2020

Comp Rev Food Sci Food Safe

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With the advance in science and technology as well as the improvement of living standards, the function of food is no longer just to meet the needs of survival. Food science and its associated nutritional health issues have been increasingly debated. Lipids, as complex metabolites, play a key role both in food and human health. Taking advantages of mass spectrometry (MS) by combining its high sensitivity and accuracy with extensive selective determination of all lipid classes, MS‐based lipidomics has been employed to resolve the conundrum of addressing both qualitative and quantitative aspects of high‐abundance and low‐abundance lipids in complex food matrices. In this review, we systematically summarize current applications of MS‐based lipidomics in food field. First, common MS‐based lipidomics procedures are described. Second, the applications of MS‐based lipidomics in food science, including lipid composition characterization, adulteration, traceability, and other issues, are discussed. Third, the application of MS‐based lipidomics for nutritional health covering the influence of food on health and disease is introduced. Finally, future research trends and challenges are proposed. MS‐based lipidomics plays an important role in the field of food science, promoting continuous development of food science and integration of food knowledge with other disciplines. New methods of MS‐based lipidomics have been developed to improve accuracy and sensitivity of lipid analysis in food samples. These developments offer the possibility to fully characterize lipids in food samples, identify novel functional lipids, and better understand the role of food in promoting healt.

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A new dilution-enrichment sample preparation strategy for expanded metabolome monitoring of human breast milk that overcomes the simultaneous presence of low- and high-abundance lipid species

Cited by 23 publications

References 35 publications

Nuclear Magnetic Resonance Metabolomics Reveals Qualitative and Quantitative Differences in the Composition of Human Breast Milk and Milk Formulas

Nuclear Magnetic Resonance Metabolomics Reveals Qualitative and Quantitative Differences in the Composition of Human Breast Milk and Milk Formulas

Comparative Lipidomic Study of Human Milk from Different Lactation Stages and Milk Formulas

Mass spectrometry‐based lipidomics in food science and nutritional health: A comprehensive review

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