A New Device for Endothelial Cell Seeding of a Small‐Caliber Vascular Prosthesis

Mazzucotelli, J. P.; Roudière, Jean Louis; Bernex, Florence; Bertrand, Patrick; Lèandri, J; Loisance, D

doi:10.1111/j.1525-1594.1993.tb00632.x

Cited by 26 publications

(10 citation statements)

References 8 publications

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“…Desirable features for any seeding technique include: preserving cell viability, providing a uniform cell distribution, and attaining a high seeding efficiency, with reduced seeding time [38,39]. The vast majority of seeding techniques for vascular tissue engineering involve surface seeding [51][52][53][54]. Bulk seeding is harder to accomplish (particularly in tubular constructs) and usually requires an active method of deployment [16,39].…”

Section: Discussionmentioning

confidence: 99%

Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique

et al. 2008

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There is a clinical need for a tissue-engineered vascular graft (TEVG), and combining stem cells with biodegradable tubular scaffolds appears to be a promising approach. The goal of this study was to characterize the incorporation of muscle-derived stem cells (MDSCs) within tubular poly(ester urethane) urea (PEUU) scaffolds in vitro to understand their interaction, and to evaluate the mechanical properties of the constructs for vascular applications. Porous PEUU scaffolds were seeded with MDSCs using our recently described rotational vacuum seeding device, and cultured inside a spinner flask for 3 or 7 days. Cell viability, number, distribution and phenotype were assessed along with the suture retention strength and uniaxial mechanical behavior of the TEVGs. The seeding device allowed rapid even distribution of cells within the scaffolds. After 3 days, the constructs appeared completely populated with cells that were spread within the polymer. Cells underwent a population doubling of 2.1-fold, with a population doubling time of 35 h. Stem cell antigen-1 (Sca-1) expression by the cells remained high after 7 days in culture (77+/-20% vs. 66+/-6% at day 0) while CD34 expression was reduced (19+/-12% vs. 61+/-10% at day 0) and myosin heavy chain expression was scarce (not quantified). The estimated burst strength of the TEVG constructs was 2127+/-900 mm Hg and suture retention strength was 1.3+/-0.3N. We conclude from this study that MDSCs can be rapidly seeded within porous biodegradable tubular scaffolds while maintaining cell viability and high proliferation rates and without losing stem cell phenotype for up to 7 days of in-vitro culture. The successful integration of these steps is thought necessary to provide rapid availability of TEVGs, which is essential for clinical translation.

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Section: Discussionmentioning

confidence: 99%

Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique

et al. 2008

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“…Cell seeding on a solid support proceeds generally by either static or dynamic seeding. 1415,16 In previous studies, dynamic cell seeding resulted in more efficient and homogeneous cell seeding compared to static seeding. 17…”

Section: Introductionmentioning

confidence: 95%

A Simple Dynamic Strategy to Deliver Stem Cells to Decellularized Nerve Allografts

Rbia¹,

Bulstra²,

Bishop³

et al. 2018

Plastic &Amp; Reconstructive Surgery

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“…41, 60 Numerous techniques have been developed since then with the goal of enhancing EC coverage and retention on synthetic grafts such as cell settling, 16,25,33 hydrostatic delivery, 28,56,61 electrostatic attraction, 7,15 and pre-coating/surface modification. 2,27,29,42,48,50 While these techniques have improved short-term graft performance with varying degrees of success, long-term EC retention remains problematic for some of these approaches.…”

Section: Introductionmentioning

confidence: 99%

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

2006

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It is generally accepted that endothelialization and subsequent development of a functional endothelium are of paramount importance to the success of any bioartificial artery. In this study, we aimed to assess the ability of smooth muscle cell-remodeled, fibrin-based media-equivalents (MEs) to be endothelialized, examine the morphological changes of endothelial cells (ECs) associated with exposure to physiologically-relevant shear stress in a custom-built bioreactor, and determine if adherent ECs are capable of withstanding average physiological shear stresses. It was found that MEs could be readily endothelialized with surface coverages of 98.8 +/- 0.9% after two days, and the ECs expressed von Willebrand factor. Furthermore, EC retention remained high (steady: 96.5 +/- 4.4%, pulsatile: 94.3 +/- 4.3%) under exposure to physiologically relevant shear stresses for 48 h. The results indicate that these MEs are conducive to generating an EC monolayer, with the ECs possessing adhesion strength sufficient to withstand physiological shear stress and maintain a normal phenotype.

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A New Device for Endothelial Cell Seeding of a Small‐Caliber Vascular Prosthesis

Cited by 26 publications

References 8 publications

Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique

Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique

A Simple Dynamic Strategy to Deliver Stem Cells to Decellularized Nerve Allografts

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

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