“…We define ΔIROP (%) as the difference between the reference IROP and the IROP after recognition of the biological event. Therefore, this methodology allows us to detect the concentration of the target biomolecules in an analyzed sample after specific binding to the biosensing response, as previously reported [ 37 , 43 ]. Fig.…”
Section: Methodsmentioning
confidence: 82%
“…Not only are the biosensors important, but also is the manner in which the transducing signals are readout in them. The interferometric optical detection method has been reported [ 37 , 38 ] to be a reliable system to archive the demanding limit of detection required for most in vitro diagnostic systems. It also has capacity for high-throughput screening.…”
The standard rapid approach for the diagnosis of coronavirus disease 2019 (COVID-19) is the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The detection of specific anti-SARS-CoV-2 immunoglobulins is crucial for screening people who have been exposed to the virus, whether or not they presented symptoms. Recent publications report different methods for the detection of specific IgGs, IgMs, and IgAs against SARS-CoV-2; these methods mainly detect immunoglobulins in the serum using conventional techniques such as rapid lateral flow tests or enzyme-linked immunosorbent assay (ELISA). In this article, we report the production of recombinant SARS-CoV-2 spike protein and the development of a rapid, reliable, cost-effective test, capable of detecting immunoglobulins in serum and saliva samples. This method is based on interferometric optical detection. The results obtained using this method and those obtained using ELISA were compared. Owing to its low cost and simplicity, this test can be used periodically for the early detection, surveillance, detection of immunity, and control of the spread of COVID-19.
“…We define ΔIROP (%) as the difference between the reference IROP and the IROP after recognition of the biological event. Therefore, this methodology allows us to detect the concentration of the target biomolecules in an analyzed sample after specific binding to the biosensing response, as previously reported [ 37 , 43 ]. Fig.…”
Section: Methodsmentioning
confidence: 82%
“…Not only are the biosensors important, but also is the manner in which the transducing signals are readout in them. The interferometric optical detection method has been reported [ 37 , 38 ] to be a reliable system to archive the demanding limit of detection required for most in vitro diagnostic systems. It also has capacity for high-throughput screening.…”
The standard rapid approach for the diagnosis of coronavirus disease 2019 (COVID-19) is the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The detection of specific anti-SARS-CoV-2 immunoglobulins is crucial for screening people who have been exposed to the virus, whether or not they presented symptoms. Recent publications report different methods for the detection of specific IgGs, IgMs, and IgAs against SARS-CoV-2; these methods mainly detect immunoglobulins in the serum using conventional techniques such as rapid lateral flow tests or enzyme-linked immunosorbent assay (ELISA). In this article, we report the production of recombinant SARS-CoV-2 spike protein and the development of a rapid, reliable, cost-effective test, capable of detecting immunoglobulins in serum and saliva samples. This method is based on interferometric optical detection. The results obtained using this method and those obtained using ELISA were compared. Owing to its low cost and simplicity, this test can be used periodically for the early detection, surveillance, detection of immunity, and control of the spread of COVID-19.
“…The read-out methodology for this point-of-care device is based on the increase relative optical power (IROP) value, previously introduced in several publications [ 27 , 28 , 30 ], and defined as the quotient of the optical power of two interferometers: signal (I Sig ) and reference (I Ref ) interferometers in a certain optical band, given by the light source employed ( Figure 2 ).…”
Section: Methodsmentioning
confidence: 99%
“…Our research group has developed a point-of-care device based on optical label-free detection. The device is a compact and cost-effective point-of-care based on the “increase relative optical power” (IROP) principle and the enhancement in performance and LoD in comparison with standard high-resolution spectrometry [ 27 , 28 , 29 , 30 ]. The “increase relative optical power” quantifies the biomolecule accumulation or recognition in one sensing surface.…”
Interferon-gamma (IFN-γ) is a cytokine associated with inflammatory diseases, virus, infection, etc. The quantification of interferon-gamma concentration levels is studied to relate the immune system response to the progression of disease. In this work, we used a label-free point-of-care device based on the increase relative optical power (IROP) and a biosensor based on photonic transducers called BICELLs (Biophotonic Sensing Cells) to evaluate interferon-gamma concentrations. The BICELLs’ sensing surface size used is 100 μm in diameter. The bioreceptor is attached to the surface by streptavidin-biotin affinity. This label-free IROP-based device can work with a low concentration of reagents and a low sample volume for measurements. Furthermore, this new device was compared with an ELISA technique in the same conditions. A good correlation was achieved between both techniques. This device is easy to use, and it is a cost-effective tool for monitoring an analyte in a disease.
“…The combination of the measurement of the optical intensity in a given spectral range with the interferometry in two interferometric sensing cells is a method to enhance the biosensing system as a whole. This interferometric optical detection method (IODM) has been detailed elsewhere [58][59][60], has been patented [61], and is under commercial exploitation by bio optical detection (BioD) [62].…”
In this work, we review the technology of vertically interrogated optical biosensors from the point of view of engineering. Vertical sensors present several advantages in the fabrication processes and in the light coupling systems, compared with other interferometric sensors. Four different interrelated aspects of the design are identified and described: sensing cell design, optical techniques used in the interrogation, fabrication processes, fluidics, and biofunctionalization of the sensing surface. The designer of a vertical sensor should decide carefully which solution to adopt on each aspect to finally integrating all the components in a single platform. Complexity, cost, and reliability of this platform will be determined by the decisions taken on each of the design process. We focus in the research and experience acquired by our group during last years on the field of optical biosensors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
