Engineering vertically interrogated interferometric sensors for optical label-free biosensing

Casquel, Rafael; Holgado, Miguel; Heras, María Fe Laguna; Hernández, Ana López; Santamaría, Beatriz; Lavín, Álvaro; Tramarin, Luca; Herreros, Pedro

doi:10.1007/s00216-020-02411-3

Cited by 15 publications

(15 citation statements)

References 68 publications

(89 reference statements)

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“…The latter two are based on labeled technologies such as enzyme-linked immunosorbent assay (ELISA) or lateral flow-based systems [ 16 ]. However, the significant advantages of optical biosensing systems based on combined interferometry and resonance, used with or without optical waveguides, are worth mentioning [ 17 , 18 ]. The applications of surface plasmon resonance-based biosensors [ [19] , [20] , [21] ], diffraction grating coupled interferometry [ 22 ], photonic crystals [ 23 , 24 ], ring resonators [ [25] , [26] , [27] ], Mach–Zehnder [ 28 , 29 ], guided-mode resonance biosensors [ 30 , 31 ], Young interferometers [ 32 ], and resonant nanopillars [ 33 ], among others, have been widely reported.…”

Section: Introductionmentioning

confidence: 99%

Developing an Optical Interferometric Detection Method based biosensor for detecting specific SARS-CoV-2 immunoglobulins in Serum and Saliva, and their corresponding ELISA correlation

Murillo

Tomé-Amat

Ramírez

et al. 2021

Sensors and Actuators B: Chemical

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The standard rapid approach for the diagnosis of coronavirus disease 2019 (COVID-19) is the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The detection of specific anti-SARS-CoV-2 immunoglobulins is crucial for screening people who have been exposed to the virus, whether or not they presented symptoms. Recent publications report different methods for the detection of specific IgGs, IgMs, and IgAs against SARS-CoV-2; these methods mainly detect immunoglobulins in the serum using conventional techniques such as rapid lateral flow tests or enzyme-linked immunosorbent assay (ELISA). In this article, we report the production of recombinant SARS-CoV-2 spike protein and the development of a rapid, reliable, cost-effective test, capable of detecting immunoglobulins in serum and saliva samples. This method is based on interferometric optical detection. The results obtained using this method and those obtained using ELISA were compared. Owing to its low cost and simplicity, this test can be used periodically for the early detection, surveillance, detection of immunity, and control of the spread of COVID-19.

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Section: Introductionmentioning

confidence: 99%

Developing an Optical Interferometric Detection Method based biosensor for detecting specific SARS-CoV-2 immunoglobulins in Serum and Saliva, and their corresponding ELISA correlation

Murillo

Tomé-Amat

Ramírez

et al. 2021

Sensors and Actuators B: Chemical

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“…There have been efforts to develop optical biosensors utilizing the simple structure of FPI to have characteristics such as an easy fabrication process, simple test setup, and cost-effectiveness [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. For example, You et al developed a microfluidic enhanced FPI-based biosensor to detect glucose, sodium chloride (NaCl), and potassium chloride (KCl) for diabetes patients [ 37 ].…”

Section: Fabry-perot Interferometer (Fpi)-based Biosensorsmentioning

confidence: 99%

Label-Free Optical Resonator-Based Biosensors

Rho

Breaux

Kim

2020

Sensors

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The demand for biosensor technology has grown drastically over the last few decades, mainly in disease diagnosis, drug development, and environmental health and safety. Optical resonator-based biosensors have been widely exploited to achieve highly sensitive, rapid, and label-free detection of biological analytes. The advancements in microfluidic and micro/nanofabrication technologies allow them to be miniaturized and simultaneously detect various analytes in a small sample volume. By virtue of these advantages and advancements, the optical resonator-based biosensor is considered a promising platform not only for general medical diagnostics but also for point-of-care applications. This review aims to provide an overview of recent progresses in label-free optical resonator-based biosensors published mostly over the last 5 years. We categorized them into Fabry-Perot interferometer-based and whispering gallery mode-based biosensors. The principles behind each biosensor are concisely introduced, and recent progresses in configurations, materials, test setup, and light confinement methods are described. Finally, the current challenges and future research topics of the optical resonator-based biosensor are discussed.

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“…For each of the steps, we report different characterization techniques, CA (Contact Angle), ellipsometry, XPS (X-ray photoelectron spectroscopy), fluorescence, that indicate the effectiveness of the described biofunctionalization process. Among the various approaches for label-free optical biosensing, including surface plasmon resonance devices, ring resonators, photonic crystals, and Mach-Zehnder devices [16][17][18][19], we used an interferometric optical biosensing based on vertical interrogation [20,21].…”

Section: Introductionmentioning

confidence: 99%

Efficient Chemical Surface Modification Protocol on SiO2 Transducers Applied to MMP9 Biosensing

Hernández

Pujari

Heras

et al. 2021

Sensors

Self Cite

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The bioreceptor immobilization process (biofunctionalization) turns to be one of the bottlenecks when developing a competent and high sensitivity label-free biosensor. Classical approaches seem to be effective but not efficient. Although biosensing capacities are shown in many cases, the performance of the biosensor is truncated by the inefficacious biofunctionalization protocol and the lack of reproducibility. In this work, we describe a unique biofunctionalization protocol based on chemical surface modification through silane chemistry on SiO2 optical sensing transducers. Even though silane chemistry is commonly used for sensing applications, here we present a different mode of operation, applying an unusual silane compound used for this purpose (3-Ethoxydimethylsilyl)propylamine, APDMS, able to create ordered monolayers, and minimizing fouling events. To endorse this protocol as a feasible method for biofunctionalization, we performed multiple surface characterization techniques after all the process steps: Contact angle (CA), X-ray photoelectron spectroscopy (XPS), ellipsometry, and fluorescence microscopy. Finally, to evidence the outputs from the SiO2 surface characterization, we used those SiO2 surfaces as optical transducers for the label-free biosensing of matrix metalloproteinase 9 (MMP9). We found and demonstrated that the originally designed protocol is reproducible, stable, and suitable for SiO2-based optical sensing transducers.

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Engineering vertically interrogated interferometric sensors for optical label-free biosensing

Cited by 15 publications

References 68 publications

Developing an Optical Interferometric Detection Method based biosensor for detecting specific SARS-CoV-2 immunoglobulins in Serum and Saliva, and their corresponding ELISA correlation

Developing an Optical Interferometric Detection Method based biosensor for detecting specific SARS-CoV-2 immunoglobulins in Serum and Saliva, and their corresponding ELISA correlation

Label-Free Optical Resonator-Based Biosensors

Efficient Chemical Surface Modification Protocol on SiO2 Transducers Applied to MMP9 Biosensing

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