A new cultivation system for studying chemical effects on the lifespan of the fruit fly

Li, Shuguang; Chen, Ke; Li, Xin; Zhang, Xinwen; Liu, Shi V.

doi:10.1016/j.exger.2009.11.004

Cited by 10 publications

(7 citation statements)

References 22 publications

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“…Drosophila melanogaster is an excellent model for the study of aging because adults show many similarities with the cellular senescence observed in mammals [ 77 ]. This is the reason why this particular model is frequently used to understand the relationship between nutrient metabolism and aging mechanisms [ 25 ]. To our knowledge, the antiageing and antidegenerative effects of CCC and CFCC were assayed for the first time using D. melanogaster in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Somatic cell mutations and apoptosis-resistance, widely associated with genetic toxicity and carcinogenicity, are frequently assayed using the in vivo Drosophila melanogaster model through the Somatic Mutation and Recombination Test (SMART) [ 20 , 21 ], which was demonstrated as a reliable assay to detect genotoxic and antigenotoxic activity of single compounds and complex mixtures [ 22 , 23 ]. More recently, this fly model was also increasingly used to study life extension since there is a high homology between invertebrate and human genes involved in aging process [ 24 , 25 ]. On the other hand, the determination of cytotoxicity, DNA internucleosomal fragmentation, and DNA single/double strand breaks in HL-60 promyelocytic cells is also used as a first step to detect toxicity, necrosis, and apoptosis in chemoprevention processes [ 26 – 28 ].…”

Section: Introductionmentioning

confidence: 99%

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In VivoandIn VitroGenotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Mateo-Fernández

Merinas-Amo

Moreno-Millán

et al. 2016

BioMed Research International

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The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola™ (CCC) and Caffeine-Free Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In VivoandIn VitroGenotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Mateo-Fernández

Merinas-Amo

Moreno-Millán

et al. 2016

BioMed Research International

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“…In vivo antitoxicology assays have been performed through time in order to ascertain the health-promoting properties of the tested compounds. D. melanogaster model is increasingly used to study life extension, since there is a high homology between invertebrate and human genes involved in the aging process [17,49].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Drosophila melanogaster is an excellent model for the study of aging, because adults show many similarities with the cellular senescence observed in mammals [16]. This is the reason why this particular model is frequently used to understand the relationship between nutrient metabolism and aging mechanisms [17], and further substantial contributions in this sense are expected [18].…”

Section: Introductionmentioning

confidence: 99%

Food Safety and Nutraceutical Potential of Caramel Colour Class IV Using In Vivo and In Vitro Assays

Mateo-Fernández

Alves-Martínez

Río-Celestino

et al. 2019

Foods

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Nutraceutical activity of food is analysed to promote the healthy characteristics of diet where additives are highly used. Caramel is one of the most worldwide consumed additives and it is produced by heating natural carbohydrates. The aim of this study was to evaluate the food safety and the possible nutraceutical potential of caramel colour class IV (CAR). For this purpose, in vivo toxicity/antitoxicity, genotoxicity/antigenotoxicity and longevity assays were performed using the Drosophila melanogaster model. In addition, cytotoxicity, internucleosomal DNA fragmentation, single cell gel electrophoresis and methylation status assays were conducted in the in vitro HL-60 human leukaemia cell line. Our results reported that CAR was neither toxic nor genotoxic and showed antigenotoxic effects in Drosophila. Furthermore, CAR induced cytotoxicity and hipomethylated sat-α repetitive element using HL-60 cell line. In conclusion, the food safety of CAR was demonstrated, since Lethal Dose 50 (LD50) was not reached in toxicity assay and any of the tested concentrations induced mutation rates higher than that of the concurrent control in D. melanogaster. On the other hand, CAR protected DNA from oxidative stress provided by hydrogen peroxide in Drosophila. Moreover, CAR showed chemopreventive activity and modified the methylation status of HL-60 cell line. Nevertheless, much more information about the mechanisms of gene therapies related to epigenetic modulation by food is necessary.

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“…This method quickly detects any influence of toxins on metabolism [31,39]. The concentrations we used for raising flies (from 20 to 2 mM) were chosen based on previous reports [40,41], and normal-fed males were used as controls. Raising Drosophila on food containing 60, 200 or 600 lM DEHP made them significantly more starvation resistant (p < 0.001 for all the three groups) while the highest and lowest concentrations were similar to the normal-fed flies ( fig.…”

Section: Dehp Exposure Increases Starvation Resistancementioning

confidence: 99%

Bis‐(2‐ethylhexyl) Phthalate Increases Insulin Expression and Lipid Levels in Drosophila melanogaster

Cao

Wiemerslage

Marttila

et al. 2016

Basic Clin Pharma Tox

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Bis-(2-ethylhexyl) phthalate (DEHP) is one of the most widely used plasticizers, and human beings are exposed to DEHP via polyvinyl chloride (PVC) materials, medical equipment and even drinking water. While DEHP has been implicated to influence metabolism and endocrine functions, important questions remain about the molecular mechanisms of these effects. We employed the model organism Drosophila melanogaster and examined physiological, molecular and behavioural effects from DEHP-contaminated food. We found that DEHP, at levels comparable to human exposure, made male flies more resistant to starvation and increased lipid levels, while decreasing circulating carbohydrates. Moreover, DEHP-fed male flies had higher expression levels of an insulin-like peptide known to regulate metabolism, as well as the insulin receptor. Our results suggest that longterm DEHP feeding may induce diabetes-like dysfunctions. These findings provide a molecular background of how DEHP may have detrimental effects on metabolic functions.Bis-(2-ethylhexyl) phthalate (DEHP) is one of the most widely used plasticizers in manufacturing polyvinyl chloride (PVC) products [1]. Marked leaching into the environment occurs because phthalates are not covalently bound to the polymeric matrix. Human exposure to DEHP can occur via many routes, such as medical equipment, food containers and beverages, including drinking water. Although there are some limitations of using DEHP in food and devices, human exposure to excess DEHP is common. According to the United States Food and Drug Administration (FDA), medical devices made with PVC release DEHP into the body at levels up to 10 times higher than the tolerable daily intake (TDI) level [2]. In 2010, a review by the European Chemicals Agency (ECHA) announced that high levels of DEHP metabolites were detected in breastmilk, urine and other body fluid samples [3][4][5], while a survey conducted in Germany showed approximately 12% of the population exceeded the TDI value [6]. Thus, investigating how DEHP may disrupt metabolism is an important human health issue.Chronic exposure to DEHP and its oxidized metabolites may disrupt metabolism [7]. A study in rats found evidence that DEHP causes insulin resistance in adipose tissue via oxidation of glucose [8]. Studies also suggest that exposure to DEHP may affect mammalian reproductive ability [9-11] and development [12,13]. In human beings, DEHP metabolites, such as mono(2-ethyl-5-oxo-hexyl) phthalate (5oxo-MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (5OH-MEHP) and mono (2-ethyl-5-hydroxyhexyl) phthalate (6-OH-MEHP), which increase in urine with age and weight [14], positively correlate with obesity and insulin resistance [15,16].Importantly, in children aged less than 3 months, the glucuronidation pathway is still immature, which may cause increased internal concentrations of these toxic metabolites [17,18].However, it is unclear how DEHP interferes with metabolic pathways. To investigate the disruptive effects of DEHP, we employed Drosophila melanogast...

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A new cultivation system for studying chemical effects on the lifespan of the fruit fly

Cited by 10 publications

References 22 publications

In VivoandIn VitroGenotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

In VivoandIn VitroGenotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Food Safety and Nutraceutical Potential of Caramel Colour Class IV Using In Vivo and In Vitro Assays

Bis‐(2‐ethylhexyl) Phthalate Increases Insulin Expression and Lipid Levels in Drosophila melanogaster

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