<i>In Vivo</i>and<i>In Vitro</i>Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Mateo-Fernández, M.; Merinas-Amo, Tania; Moreno-Millán, M.; Alonso-Moraga, Ángeles; Demyda-Peyrás, S.

doi:10.1155/2016/7574843

Cited by 16 publications

(18 citation statements)

References 101 publications

(122 reference statements)

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“…It was demonstrated that more than 70% of human disease-causing genes have a functional homolog in this fly model [69]. Additionally, this particular model was also largely used to evaluate the genotoxicity of different biological compounds and molecules due to its accuracy, robustness, and reproducibility [70,71,72].…”

Section: Discussionmentioning

confidence: 99%

Nutraceutic Potential of Two Allium Species and Their Distinctive Organosulfur Compounds: A Multi-Assay Evaluation

Fernández-Bedmar

Demyda-Peyrás

Merinas-Amo

et al. 2019

Foods

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This study aimed to evaluate the biological activities of two Allium species (garlic and onion) as well as diallyl disulphide (DADS) and dipropyl disulphide (DPDS) as their representative bioactive compounds in a multi-assay experimental design. The genotoxic, antigenotoxic, and lifespan effects of garlic, onion, DADS, and DPDS were checked in Drosophila melanogaster and their cytotoxic, pro-apoptotic, and DNA-clastogenic activities were analyzed using HL60 tumoral cells. All compounds were non-genotoxic and antigenotoxic against H2O2-induced DNA damage with a positive dose-response effect and different inhibition percentages (the highest value: 95% for DADS) at all tested concentrations. Daily intake of Allium vegetables, DADS, or DPDS had no positive effects on flies’ lifespan and health span. Garlic and DADS exerted the highest cytotoxic effects in a positive dose-dependent manner. Garlic and DADS exerted a DNA-internucleosomal fragmentation as an index of induced proapoptotic activity on HL60 cells. Allium vegetables and DADS were able to induce clastogenic strand breaks in the DNA of HL60 cells. This study showed the genomic safety of the assayed substances and their protective genetic effects against the hydrogen peroxide genotoxine. Long-term treatments during the whole life of the Drosophila genetic model were beneficial only at low-median concentrations. The chemo-preventive activity of garlic could be associated with its distinctive organosulfur DADS. We suggest that supplementary studies are needed to clarify the cell death pathway against garlic and DADS.

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Section: Discussionmentioning

confidence: 99%

Nutraceutic Potential of Two Allium Species and Their Distinctive Organosulfur Compounds: A Multi-Assay Evaluation

Fernández-Bedmar

Demyda-Peyrás

Merinas-Amo

et al. 2019

Foods

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“…After completing the washing steps in PBS (Phosphated-Buffered Saline), 6.25 × 10 5 cells/mL were mixed with low melting point agarose (Sigma, A4018) at 0.75% and immediately transferred to frosted-end slides. Next, according to the Mateo-Fernández et al [43] protocol, the steps of lysis, alkaline electrophoresis, neutralization and drying were carried out. Finally, 50–100 single cells were visualized by treating the slides with 7 µL of propidium iodide (10 µg/mL) (Sigma, P4170).…”

Section: Methodsmentioning

confidence: 99%

Toxicological Studies of Czech Beers and Their Constituents

Merinas-Amo

García-Zorrilla

et al. 2019

Foods

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Background: Czech beers are unique because they are brewed using specific technology at a particular latitude and for being entirely produced in the area of the Czech Republic. The purpose of this work is the evaluation of toxicological effects of a variety of freeze-dried Czech beers, their raw materials (malts, hops and yeast) and processed-beer (wort, hopped wort and young beer). Methods: In vivo assays to evaluate the safety and protective effects in the Drosophila melanogaster eukaryotic system, and the in vitro evaluations of chemopreventive and DNA damage activity using the HL-60 tumour human cell line were carried out. Results: The safe effects for all the analysed substances and general protective effects against H2O2 were shown both at the individual and genomic level in the Drosophila animal model, with some exceptions. Moreover, all the substances were able to inhibit the tumour cell growth and to induce DNA damage in the HL-60 cells at different levels (proapoptotic, single/double strands breaks and methylation status). Conclusions: The promising effects shown by freeze-dried Czech beers due to their safety, protection against a toxin, chemopreventive potential and the induction of DNA damage in tumour cells, allow the proposition of Czech beer as a beverage with nutraceutic potential.

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“…The DNA breakage ability of the compounds was assessed by the alkaline comet assay according to Mateo‐Fernández et al []. HL60 cells (5 × 10 5 cells) were treated with different tested concentration of tomato (0.125, 0.25, and 0.5 mg/mL) and lycopene (1.2, 2.4, and 4.8 µM) for 5 hr.…”

Section: Methodsmentioning

confidence: 99%

Anti/genotoxic, longevity inductive, cytotoxic, and clastogenic‐related bioactivities of tomato and lycopene

Fernández-Bedmar

Anter

Moraga

2018

Environ and Mol Mutagen

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The aim of this study was to evaluate some biological activities of tomato as well as lycopene and to consider a new nutraceutic value for this fruit regarding to the protection against genetic damage and as a chemopreventive agent. Genotoxicity, DNA-protection against hydrogen peroxide, and lifespan properties of tomato and lycopene were assessed through wing spot test and longevity assay using the Drosophila in vivo model. Additionally, chemopreventive activity was investigated through cytotoxicity, DNA-fragmentation comet and annexin V FITC/PI assays using HL60 in vitro model. Results showed that: (i) tomato and lycopene are not genotoxic and protect against H O -induced damage; (ii) with respect to the lifespan, tomato and lycopene are harmless at the lowest concentration; (iii) tomato is cytotoxic in a dose-dependent manner, but not lycopene; (iv) tomato and lycopene do not induce internucleosomal DNA-fragmentation although they induce significant clastogenic activity at low level in the leukemia cells. To sum up, tomato is a good candidate to be considered as a nutraceutical substance. Furthermore, synergistic action among other components within tomato matrix could be the cause of the health effects observed in this vegetable, which are not fully explained by lycopene. Environ. Mol. Mutagen. 59:427-437, 2018. © 2018 Wiley Periodicals, Inc.

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In VivoandIn VitroGenotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Cited by 16 publications

References 101 publications

Nutraceutic Potential of Two Allium Species and Their Distinctive Organosulfur Compounds: A Multi-Assay Evaluation

Nutraceutic Potential of Two Allium Species and Their Distinctive Organosulfur Compounds: A Multi-Assay Evaluation

Toxicological Studies of Czech Beers and Their Constituents

Anti/genotoxic, longevity inductive, cytotoxic, and clastogenic‐related bioactivities of tomato and lycopene

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