A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent

Jagota, S.K.; Dani, H. M.

doi:10.1016/0003-2697(82)90162-2

Cited by 477 publications

(243 citation statements)

References 6 publications

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“…At the end of 0, 24 or 48 hr lenses were scanned by a TCLSM. The level of VC in the incubation medium was analyzed (33). 45 Ca-Uptake and Movement into the Incubated Rat Lenses: 45 Ca (1 /xCi) was added into the lens incubation medium, M199 with or without elevated glucose (55.6 mM).…”

Section: Materials and Methods;mentioning

confidence: 99%

Modelling cortical cataractogenesis XXIX. Calpain proteolysis of lens fodrin in cataract

Kilic

Trevithick

1998

IUBMB Life

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SUMMARYThe relation between cataract and calpain proteolysis of lens fodrin was studied in two systems: elevated glucose (55.6 mM, diabetic model), and cytochalasin D (CD, 10 2 mM, actin depolymerization-induced opacity model). Glucose treatment (48 h) caused a visible opaque layer and enzyme leakage, with a concomitant accumulation of ([Ca2+]¡) around the lens equatorial cortex. CD caused both earlier and greater opacity and enzyme leakage than glucose. Lens fodrin digestion occurred in parallel with the timing and extent of calcium elevation. A calpain inhibitor peptide (CIP, 10" 2 mM) reduced the proteolysis of fodrin, opacity, and enzyme leakage in glucose-treated lenses but only partially retarded them in CDtreated lenses. These results suggest a mechanism in which calpain proteolysis of fodrin is a critical event in lens damage during opacification of cortical cataract.

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Section: Materials and Methods;mentioning

confidence: 99%

Modelling cortical cataractogenesis XXIX. Calpain proteolysis of lens fodrin in cataract

Kilic

Trevithick

1998

IUBMB Life

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“…The vitamin C concentration in the extract was quantified using Folin-Ciocalteu's phenol reagent reaction method according to procedure that was based on the methods of Omaye et al (1979);and Japota and Dani, (1982). 0.5 ml of the extract was mixed with 1.5 ml of 10% (v/v) acetic acid and 0.5 ml of Folin-Ciocalteu reagen t (1:10 dilution).…”

Section: Determination Of Total Vitamins C and E Levels In Mepgmentioning

confidence: 99%

Phytochemicals, antioxidant potentials and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of Piper guineense (Schumach Thonn) seed

Adeniyi¹,

Wilson²,

Oluboade³

2017

Afr. J. Plant Sci.

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The study involved preparation, phytochemical screening and evaluation of antioxidant potentials of the extract of Piper guineense. This was with a view to investigating the possibility of using the plant in the treatment and management of oxidant and inflammatory related disorders. Spices are herbs that contain complex mixtures of phytochemicals which give characteristic odours, flavours and protect living systems from deleterious effect of reactive oxygen species (ROS). Phytochemical screening of the plant revealed the presence of alkaloids, flavonoids, tannins, cardiac glycosides, triterpenoids and saponins. The extract exhibited DPPH-free radical scavenging activity which was concentration dependent. The results revealed that the extract is rich in antioxidant components which make the seed useful in the prevention and management of various forms of diseases.

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“…Recently, P-Sb-Mo heteropoly acid 91 has been used to produce heteropoly blue (λ max =710 nm) for the assay of ascorbic acid over the range 1 -50 µg ml -1 (ε=3.68×10 3 ). The use of folin reagent 92 and folin phenol 93 (λ max =760 nm) has also been described for the assay of biological samples after deproteinizing with TCA. Beer's law is obeyed up to 45 µg ml -1 .…”

Section: Resultsmentioning

confidence: 99%

Photometric Methods for the Determination of Vitamin C

1998

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A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent

Cited by 477 publications

References 6 publications

Modelling cortical cataractogenesis XXIX. Calpain proteolysis of lens fodrin in cataract

Modelling cortical cataractogenesis XXIX. Calpain proteolysis of lens fodrin in cataract

Phytochemicals, antioxidant potentials and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of Piper guineense (Schumach Thonn) seed

Photometric Methods for the Determination of Vitamin C

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