A New Cold-Induced Alfalfa Gene Is Associated with Enhanced Hardening at Subzero Temperature

Monroy, Antonio F.; Castonguay, Yves; Laberge, Serge; Sarhan, Fathey; Vézina, Louis‐P.; Dhindsa, Rajinder S.

doi:10.1104/pp.102.3.873

Cited by 178 publications

(114 citation statements)

References 25 publications

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“…MtCAS15 (Medtr7g086340) is induced by low temperature in Medicago spp., and a positive correlation between freezing tolerance and its expression level was identified in Medicago spp. (Monroy et al, 1993a;Pennycooke et al, 2008;Zhang et al, 2011). In addition, MtLTI65 (AC235674_16) and MtY3G1 (Medtr2g014050; a late embryogenesis abundant protein) also were reported to participate in cold stress in previous studies (Nordin et al, 1993;Hincha and Thalhammer, 2012).…”

Section: Mtcbf4 Regulates Genes Linked To Stresses and Developmentmentioning

confidence: 99%

“…Two MYB TFs, GmMYB76 and GmMYB177, also were shown to enhance freezing tolerance in Arabidopsis by inducing the expression of freezing tolerance-responsive genes (Liao et al, 2008). In alfalfa (Medicago sativa), cold induces a calcium influx, which affects protein phosphorylation and the expression levels of cold acclimation-specific genes such as CAS15 and CAS18 (Mohapatra et al, 1989;Monroy et al, 1993aMonroy et al, , 1993bWolfraim et al, 1993;Monroy and Dhindsa, 1995). In M. truncatula and Medicago falcata, cold induces the expression of CBF1 to CBF3 and CAS genes (Pennycooke et al, 2008;Zhang et al, 2011).…”

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confidence: 99%

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Opposing control by transcription factors MYB61 and MYB3 Increases Freezing Tolerance by relieving C-repeat Binding Factor suppression

Zhang

et al. 2016

Plant Physiol.

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ORCID IDs: 0000-0003-0210-9408 (Z.Z.); 0000-0002-6839-3272 (X.H.); 0000-0001-5113-7750 (J.W.); 0000-0002-5098-8681 (J.D.).Cold acclimation is an important process by which plants respond to low temperature and enhance their winter hardiness. C-REPEAT BINDING FACTOR1 (CBF1), CBF2, and CBF3 genes were shown previously to participate in cold acclimation in Medicago truncatula. In addition, MtCBF4 is transcriptionally induced by salt, drought, and cold stresses. We show here that MtCBF4, shown previously to enhance drought and salt tolerance, also positively regulates cold acclimation and freezing tolerance. To identify molecular factors acting upstream and downstream of the MtCBF4 transcription factor (TF) in cold responses, we first identified genes that are differentially regulated upon MtCBF4 overexpression using RNAseq Digital Gene Expression Profiling. Among these, we showed that MtCBF4 directly activates the transcription of the COLD ACCLIMATION SPECIFIC15 (MtCAS15) gene. To gain insights into how MtCBF4 is transcriptionally regulated in response to cold, an R2R3-MYB TF, MtMYB3, was identified based on a yeast one-hybrid screen as binding directly to MYB cis-elements in the MtCBF4 promoter, leading to the inhibition of MtCBF4 expression. In addition, another MYB TF, MtMYB61, identified as an interactor of MtMYB3, can relieve the inhibitory effect of MtMYB3 on MtCBF4 transcription. This study, therefore, supports a model describing how MtCBF4 is regulated by antagonistic MtMYB3/MtMYB61 TFs, leading to the up-regulation of downstream targets such as MtCAS15 acting in cold acclimation in M. truncatula.

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Section: Mtcbf4 Regulates Genes Linked To Stresses and Developmentmentioning

confidence: 99%

mentioning

confidence: 99%

Opposing control by transcription factors MYB61 and MYB3 Increases Freezing Tolerance by relieving C-repeat Binding Factor suppression

Zhang

et al. 2016

Plant Physiol.

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“…Some COR proteins, like Arabidopsis KIN1, show structural similarities to antifreeze proteins (Kurkela and Franck, 1990) and others to a HSP70 chaperon family which is known to have roles in protein transport, folding and assembly/disassembly processes (Neven et al, 1992). Although physiological function of these different classes of COR proteins remains largely unknown, some showed a positive correlation with the degree of freezing tolerance which is enhanced by cold acclimation (Monroy et al, 1993;Houde et al, 1995;Jaglo-Ottosen et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'.

et al. 2000

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“…CAregulated genes have been identified from numerous plant species including winter wheat (4 -6), barley (7,8), alfalfa (9 -11), and Arabidopsis (12)(13)(14). Positive correlations of coldinduced genes and freezing tolerance have been observed (10,15), and functions related to cold acclimation have been suggested for several genes (16,17).…”

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confidence: 99%

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

Karlson

Nakaminami

Toyomasu

et al. 2002

Journal of Biological Chemistry

144

154

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The molecular mechanisms of cold acclimation are still largely unknown; however, it has been established that overwintering plants such as winter wheat increases freeze tolerance during cold treatments. In prokaryotes, cold shock proteins are induced by temperature downshifts and have been proposed to function as RNA chaperones. A wheat cDNA encoding a putative nucleic acid-binding protein, WCSP1, was isolated and found to be homologous to the predominant CspA of Escherichia coli. The putative WCSP1 protein contains a three-domain structure consisting of an N-terminal cold shock domain with two internal conserved consensus RNA binding domains and an internal glycine-rich region, which is interspersed with three C-terminal CX 2 CX 4 HX 4 C (CCHC) zinc fingers. Each domain has been described independently within several nucleotide-binding proteins. Northern and Western blot analyses showed that WCSP1 mRNA and protein levels steadily increased during cold acclimation, respectively. WCSP1 induction was cold-specific because neither abscisic acid treatment, drought, salinity, nor heat stress induced WCSP1 expression. Nucleotide binding assays determined that WCSP1 binds ssDNA, dsDNA, and RNA homopolymers. The capacity to bind dsDNA was nearly eliminated in a mutant protein lacking C-terminal zinc fingers. Structural and expression similarities to E. coli CspA suggest that WCSP1 may be involved in gene regulation during cold acclimation.

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A New Cold-Induced Alfalfa Gene Is Associated with Enhanced Hardening at Subzero Temperature

Cited by 178 publications

References 25 publications

Opposing control by transcription factors MYB61 and MYB3 Increases Freezing Tolerance by relieving C-repeat Binding Factor suppression

Opposing control by transcription factors MYB61 and MYB3 Increases Freezing Tolerance by relieving C-repeat Binding Factor suppression

A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'.

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

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