A new class of thiolipids for the attachment of lipid bilayers on gold surfaces

Lang, Holger; Duschl, Claus; Vogel, Horst

doi:10.1021/la00013a029

Cited by 321 publications

(317 citation statements)

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“…calculated with constant refractive index, the "adlayer thickness" becomes a measure of molecule density; only at close packing it can be interpreted in terms of molecular dimensions. According to published data obtained with well-defined model systems, n = 1.45 is a reasonable value for the refractive index of lipids in a fluid state and adsorbed proteins (Lang et al, 1992(Lang et al, , 1994Spinke et al, 1992Spinke et al, , 1993Reiter et al, 1993; Terrettaz et ai., 1993) (and references therein).5 For lipids and fatty acid salts in a crystalline state, refractive indices in the range of n = 1.5 seem more appropriate (Swalen, 1986;Kooyman & Krull, 1991;Schmidt et al, 1992). But in our experiments, a crystalline state of lipids on the waveguide can be excluded (see below).…”

Section: Choice Of Refractive Index For Optical Measurementsmentioning

confidence: 85%

“…As anchor molecules, thiolipids were chosen, in order to produce similar lipid membrane systems on hydrophilic waveguides to those produced on gold by Lang et al (1994). DMPSH and DOPSH form self-assembled monolayers on gold surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…On gold surfaces, the hydrocarbon chains of gold-attached alkylthiols and thiolipids pack as closely as possible in a nearly hexagonal manner (Fenter et al, 1994;Lang et al, 1994). In the case of DOPSH, we determined optically a thickness of 21 A. X-ray diffraction analysis yielded a transmembrane distance of 44 A be- Thiolipid layers formed on waveguides reached a stable limiting structure after 30-60 min.…”

Section: Optical Measurements Of Thiolipid Layersmentioning

confidence: 97%

“…Strategies for the covalent attachment of lipid layers on optical devices have been already realized in the case of gold surfaces for SPR measurements using disulfide-bearing phospholipids (Lang et al, 1992(Lang et al, , 1994 and disulfide-functionalized amphiphilic copolymers (Erdelen et al, 1994).…”

mentioning

confidence: 99%

“…This procedure is simple, fast, and reproducible. Thiolipids with a polar spacer between phosphate and sulfur group allow for a further decoupling of the lipid layer from the solid support and provide an aqueous environment on both sides of the bilayer that is sufficiently large to accommodate the extramembranous parts of a membrane-traversing protein; this concept was realized previously on gold surfaces (Lang et al, 1992(Lang et al, , 1993(Lang et al, , 1994. Although chemical crosslinkers have been used for tethering thiol-bearing proteins and oligonucleotides to various supports (Hong et al, 1994;Lee et al, 1994), to our knowledge, no attempts have been made so far to anchor lipid bilayers to hydrophilic supports using maleimide chemistry.…”

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confidence: 99%

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Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

et al. 1995

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A new method is presented for measuring sensitively the interactions between ligands and their membrane-bound receptors in situ using integrated optics, thus avoiding the need for additional labels. Phospholipid bilayers were attached covalently to waveguides by a novel protocol, which can in principle be used with any glass-like surface.In a first step, phospholipids carrying head-group thiols were covalently immobilized onto Si02-Ti02 waveguide surfaces. This was accomplished by acylation of aminated waveguides with the heterobifunctional crosslinker N-succinimidyl-3-maleimidopropionate, followed by the formation of thioethers between the surface-grafted maleimides and the synthetic thiolipids. The surface-attached thiolipids served as hydrophobic templates and anchors for the deposition of a complete lipid bilayer either by fusion of lipid vesicles or by lipid self-assembly from mixed lipid/detergent micelles. The step-by-step lipid bilayer formation on the waveguide surface was monitored in situ by an integrated optics technique, allowing the simultaneous determination of optical thickness and one of the two refractive indices of the adsorbed organic layers. Surface coverages of 50-60% were calculated for thiolipid layers. Subsequent deposition of POPC resulted in an overall lipid layer thickness of 45-50 A , which corresponds to the thickness of a fluid bilayer membrane.Specific recognition reactions occurring at cell membrane surfaces were modeled by the incorporation of lipidanchored receptor molecules into the supported bilayer membranes. (1) The outer POPC layer was doped with biotinylated phosphatidylethanolamine. Subsequent specific binding of streptavidin was optically monitored. (2) A lipopeptide was incorporated in the outer POPC monolayer. Membrane binding of monoclonal antibodies, which were directed against the peptide moiety of the lipopeptide, was optically detected. The specific antibody binding correlated well with the lipopeptide concentration in the outer monolayer.

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Section: Choice Of Refractive Index For Optical Measurementsmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Optical Measurements Of Thiolipid Layersmentioning

confidence: 97%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

et al. 1995

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Ionenkanalsteuerung in Transmembranrezeptorproteinen: funktionelle Aktivität in Träger-fixierten Lipidmembranen

Stora¹,

Lakey²,

Vogel³

1999

Angewandte Chemie

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Über Thiolipide wird eine Lipiddoppelschicht (1) auf einem als Elektrode dienenden Goldträger (2) immobilisiert. Dies ermöglicht die Detektion der Liganden‐gesteuerten Funktionsweise des in die künstliche Membran eingelagerten natürlichen Transmembran‐Kanalproteins OmpF (3): Die Bindung einer Domäne (4) des Toxins Colicin N an OmpF wurde durch Oberflächenplasmonenresonanz‐Messungen, die daraufhin induzierte Blockierung des Kanals durch Impedanzspektroskopie nachgewiesen.

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Funktionale molekulare dünne Filme: topologische Template für die chemoselektive Bindung antigener Peptide an selbstorganisierte Monoschichten

et al. 1999

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Die Kombination von Selbstorganisation und regioselektiver Oberflächenchemie ermöglichte die Immobilisierung einer Peptid‐Erkennungsstelle 1 über ein Templat auf einer Goldoberfläche. Jeder der sieben Einzelschritte einschließlich der abschließenden funktionalen biomolekularen Erkennung wurde in situ durch oberflächensensitive Techniken kontrolliert. Die hier vorgestellte Strategie ist von allgemeiner Bedeutung für die Bildung komplexer supramolekularer Strukturen mit biologisch interessanten Funktionalitäten an Grenzflächen.

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A new class of thiolipids for the attachment of lipid bilayers on gold surfaces

Cited by 321 publications

References 3 publications

Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

Ionenkanalsteuerung in Transmembranrezeptorproteinen: funktionelle Aktivität in Träger-fixierten Lipidmembranen

Funktionale molekulare dünne Filme: topologische Template für die chemoselektive Bindung antigener Peptide an selbstorganisierte Monoschichten

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