A new approach to quantify anthracnose symptoms in inoculated Capsicum spp. fruits

Araújo, Maria do Socorro Bezerra de; Sudré, Cláudia Pombo; Graça, Gaspar Afonso da; Alencar, Antônio André da Silva; Geronimo, Ingrid Gaspar da Costa; Rodrigues, Rosana

doi:10.1007/s40858-022-00499-9

Cited by 3 publications

(2 citation statements)

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“…As a result, they are considered one of the most hazardous to agriculture. 30 In Brazil, C. scovillei is the most common species that infects field peppers and sweet peppers, causing anthracnose. Despite technological developments in manufacturing, anthracnose can still cause major economic losses.…”

Section: Discussionmentioning

confidence: 99%

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Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Resende,

de Oliveira Mello,

Zeraik

et al. 2024

Pest Management Science

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In the present study, we identified and characterized two defensin‐like peptides in an antifungal fraction obtained from Capsicum chinense pepper fruits and inhibited the growth of Colletotrichum scovillei, which causes anthracnose. AMPs were extracted from the pericarp of C. chinense peppers and subjected to ion exchange, molecular exclusion, and reversed‐phase in a high‐performance liquid chromatography system. We investigated the endogenous increase in reactive oxygen species (ROS), the loss of mitochondrial functioning, and the ultrastructure of hyphae. The peptides obtained from the G3 fraction through molecular exclusion chromatography were subsequently fractionated using reverse‐phase chromatography, resulting in the isolation of fractions F1, F2, F3, F4, and F5. The F1 fraction suppressed C. scovillei growth by 90, 70.4, and 44% at 100, 50, and 25 μg/mL, respectively. At 24 h, the IC50 and minimum inhibitory concentration were 21.5 μg/mL and 200 μg/mL, respectively. We found an increase in ROS, which may have resulted in an oxidative burst, loss of mitochondrial functioning, and cytoplasm retraction, as well as an increase in autophagic vacuoles. MS/MS analysis of the F1 fraction indicated the presence of two defensin‐like proteins, and we were able to identify the expression of three defensin sequences in our C. chinense fruit extract. The F1 fraction was also found to inhibit the activity of insect α‐amylases. In summary, the F1 fraction of C. chinense exhibits antifungal activity against a major pepper pathogen that causes anthracnose. These defensin‐like compounds are promising prospects for further research into antifungal and insecticide biotechnology applications.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 99%

“…Colletotrichum fungi are among the most commonly reported pathogenic fungi, with a wide range of hosts and levels of aggression. As a result, they are considered one of the most hazardous to agriculture 30 . In Brazil, C. scovillei is the most common species that infects field peppers and sweet peppers, causing anthracnose.…”

Section: Discussionmentioning

confidence: 99%