A New Anti-Infective Strategy to Reduce Adhesion-Mediated Virulence in Staphylococcus Aureus Affecting Surface Proteins

Abstract

Staphylococcus aureus is a flexible microbial pathogen frequently isolated from community-acquired and nosocomial infections. The use of indwelling medical devices is associated with a significant risk of infection by this bacterium which possesses a variety of virulence factors, including many toxins, and the ability to invade eukaryotic cells or to form biofilm on biotic and abiotic surfaces. The present study evaluates the anti-infective properties of serratiopeptidase, a secreted protein of Serratia marcescens, in impairing virulence-related staphylococcal properties, such as attachment to inert surfaces and adhesion/invasion on eukaryotic cells. SPEP seems to exert its action by modulating specific proteins. Proteomic studies performed on surface proteins extracted from SPEP-treated S. aureus cultures revealed that a number of proteins are affected by the treatment. Among these we found the adhesin/autolysin Atl, FnBP-A, SecA1, Sbi, EF-Tu, EF-G, and alpha-enolase. EF-Tu, EF-G and alpha-enolase are known to perform a variety of functions, depending on their cytoplasmic or surface localization. All these factors can facilitate bacterial colonization, persistence and invasion of host tissues. Our results suggest that SPEP could be developed as a potential anti-infective agent capable to hinder the entry of S. aureus into human tissues, and also impair the ability of this pathogen to form biofilm on prostheses, catheters and medical devices.

The Emerging Role of Serratiopeptidase in Oral Surgery: Literature Update

“…Thus, it has a potential antipathogenic action apart from its proteolytic activity. Serratiopeptidase modulates adhesion expression in some bacterial species and enhances antibiotic efficacy toward biofilm-forming bacteria [23][24][25]. The antibiofilm formation property of serratiopeptidase helps in enhancing antibiotic efficacy in the treatment of staphylococcal infections [26].…”

Response Sensitivity to Bacteriophages of Staphylococcus Aureus Strains Isolated From the Microbiota of the Oropharynx in Children of Irkutsk

“…Как известно, S. aureus обладает высоким коло-низационным потенциалом, высокой выживаемо-стью за счёт наличия прочной клеточной стенки, факторами персистенции [8], выраженной способ-ностью к биоплёнкообразованию [9,10]. Типовой вид -Staphylococcus aureus -является уникальным микроорганизмом, патогенные свойства которого определяются суммирующим действием факторов патогенности, токсинов [13] и инвазивных свойств этого штамма [12].…”

Evaluation of Contact Lens Multipurpose Solutions on Bacterial Biofilm Development

“…16,17 The wells of a sterile 96-well flat-bottomed polystyrene plate (Falcon) were filled with 100 mL of TSB containing or not containing the MPSs. Serial dilutions of each solution starting from 75% were tested.…”