Evaluation of Contact Lens Multipurpose Solutions on Bacterial Biofilm Development

Artini, Marco; Cellini, Andrea; Scoarughi, Gian Luca; Papa, Rosanna; Tilotta, Marco; Palma, Stefano Di; Selan, Laura

doi:10.1097/icl.0000000000000105

Cited by 17 publications

(18 citation statements)

References 22 publications

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“…Furthermore, ensuring contact lens hygiene depends not only on the method of cleaning but also the choice of solution. Hydrogen peroxide C/L cleaning solutions have been reported to be provide superior benefits over MPS solutions, 14,[15][16][17][18][19] yet the majority of respondents were unaware of this type of C/L solution which may imply that they are not being informed about all possible cleaning options by their optometrists.…”

Section: Discussionmentioning

confidence: 99%

Knowledge, attitudes and practices of contact lens use in a South African adolescent population

Khoza¹,

Moodley²,

Sokhulu³

et al. 2020

Afr H. Sci.

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Background: Contact lens usage is becoming increasingly popular amongst young people. Assessing their knowledge, attitudes and practices in relation to contact lens wear is therefore important, so that gaps in understanding or incorrect practices can be rectified to promote continued safety and success of contact lens wear. Objectives: This study aimed to assess and describe the knowledge, attitudes and practices of contact lens wearers aged 18 to 30 years, in the greater Durban area of South Africa. Methods: A semi-structured questionnaire was used to assess the knowledge, attitudes and practice of the participants. Results: Two hundred and forty six participants completed the survey. Young contact lens wearers in this sample generally had poor knowledge with regards to appropriate hygiene and contact lens wear complications. Despite wearers exhibiting a positive attitude with satisfactory hand hygiene and lens cleaning practices, important aspects such as lens case hygiene, storage and lens removal practices were found to be unsatisfactory. Compliance with after-care visits was also unsatisfactory. Conclusion: There is a need for contact lens practitioners to educate young contact lens wearers regarding appropriate contact lens-related care, to promote long term ocular health and contact lens-related safety. Keywords: Contact lens use; adolescent population; South Africa.

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Section: Discussionmentioning

confidence: 99%

Knowledge, attitudes and practices of contact lens use in a South African adolescent population

Khoza¹,

Moodley²,

Sokhulu³

et al. 2020

Afr H. Sci.

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“…Biofilm formation of Staphylococcus and Pseudomonas species was evaluated in the presence of SNB and SNP supernatants, respectively. Quantification of in vitro biofilm production was based on method previously reported ( Artini et al, 2015 ). Briefly, the wells of a sterile 96-well flat-bottomed polystyrene plate were filled with 100 μl of the appropriate medium.…”

Section: Methodsmentioning

confidence: 99%

Anti-Biofilm Activities from Marine Cold Adapted Bacteria Against Staphylococci and Pseudomonas aeruginosa

Papa¹,

Selan²,

Parrilli

et al. 2015

Front. Microbiol.

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Microbial biofilms have great negative impacts on the world’s economy and pose serious problems to industry, public health and medicine. The interest in the development of new approaches for the prevention and treatment of bacterial adhesion and biofilm formation has increased. Since, bacterial pathogens living in biofilm induce persistent chronic infections due to the resistance to antibiotics and host immune system. A viable approach should target adhesive properties without affecting bacterial vitality in order to avoid the appearance of resistant mutants. Many bacteria secrete anti-biofilm molecules that function in regulating biofilm architecture or mediating the release of cells from it during the dispersal stage of biofilm life cycle. Cold-adapted marine bacteria represent an untapped reservoir of biodiversity able to synthesize a broad range of bioactive compounds, including anti-biofilm molecules. The anti-biofilm activity of cell-free supernatants derived from sessile and planktonic cultures of cold-adapted bacteria belonging to Pseudoalteromonas, Psychrobacter, and Psychromonas species were tested against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa strains. Reported results demonstrate that we have selected supernatants, from cold-adapted marine bacteria, containing non-biocidal agents able to destabilize biofilm matrix of all tested pathogens without killing cells. A preliminary physico-chemical characterization of supernatants was also performed, and these analyses highlighted the presence of molecules of different nature that act by inhibiting biofilm formation. Some of them are also able to impair the initial attachment of the bacterial cells to the surface, thus likely containing molecules acting as anti-biofilm surfactant molecules. The described ability of cold-adapted bacteria to produce effective anti-biofilm molecules paves the way to further characterization of the most promising molecules and to test their use in combination with conventional antibiotics.

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“…Contact lens safety is dependent to a major extent on the cleaning of the lens with a solution able to limit bacteria found on the lens. Three commercial cleaning solutions were found to inhibit biofilm formation by staphylococci, including S. aureus [ 109 ].…”

Section: Keratitismentioning

confidence: 99%

The Pathogenesis of Staphylococcus aureus Eye Infections

O’Callaghan

2018

Pathogens

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Staphylococcus aureus is a major pathogen of the eye able to infect the tear duct, eyelid, conjunctiva, cornea, anterior and posterior chambers, and the vitreous chamber. Of these infections, those involving the cornea (keratitis) or the inner chambers of the eye (endophthalmitis) are the most threatening because of their potential to cause a loss in visual acuity or even blindness. Each of these ocular sites is protected by the constitutive expression of a variety of antimicrobial factors and these defenses are augmented by a protective host response to the organism. Such infections often involve a predisposing factor that weakens the defenses, such as the use of contact lenses prior to the development of bacterial keratitis or, for endophthalmitis, the trauma caused by cataract surgery or intravitreal injection. The structural carbohydrates of the bacterial surface induce an inflammatory response able to reduce the bacterial load, but contribute to the tissue damage. A variety of bacterial secreted proteins including alpha-toxin, beta-toxin, gamma-toxin, Panton-Valentine leukocidin and other two-component leukocidins mediate tissue damage and contribute to the induction of the inflammatory response. Quantitative animal models of keratitis and endophthalmitis have provided insights into the S. aureus virulence and host factors active in limiting such infections.

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Evaluation of Contact Lens Multipurpose Solutions on Bacterial Biofilm Development

Abstract: Our findings indicate that the CL solutions tested were all able to reduce biofilm formation. Furthermore, the BioFlux system was proven to be useful for the evaluation of the effectiveness of CL solutions against microbial biofilm formation.

Cited by 17 publications

References 22 publications

Knowledge, attitudes and practices of contact lens use in a South African adolescent population

Knowledge, attitudes and practices of contact lens use in a South African adolescent population

Anti-Biofilm Activities from Marine Cold Adapted Bacteria Against Staphylococci and Pseudomonas aeruginosa

The Pathogenesis of Staphylococcus aureus Eye Infections

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