A new anesthetic protocol to medullary nerve roots access in rats

Santos, Deivid Ramos dos; Teixeira, Renan Kleber Costa; Araújo, Nayara Pontes de; Calvo, Faustino Chaves; Duarte, Tiago Braga; Ataíde, Letícia Amanda Pinheiro de; Chaves, Rosa Helena de Figueiredo; Barros, Rui Sergio Monteiro de

doi:10.1590/acb360908

Cited by 4 publications

(7 citation statements)

References 25 publications

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“…Anesthesia for subsequent knee infiltration consisted of intraperitoneal injection of ketamine and xylazine at doses of 70 mg/kg and 7 mg/kg, respectively. 14 A booster, if required, consisted of an intramuscular application of ¼ of the initial dose.…”

Section: Methodsmentioning

confidence: 99%

Os efeitos do colágeno hidrolisado e do peptídeo de colágeno no tratamento de lesões condrais superficiais: Um estudo experimental

Santos

Xavier

Ataíde

et al. 2022

Rev Bras Ortop (Sao Paulo)

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Resumo Objetivo Avaliar os efeitos do colágeno hidrolisado e do peptídeo de colágeno no tratamento de lesões condrais superficiais de ratos. Método Foram utilizados 18 Rattus norvegicus nesta pesquisa. O dano articular foi induzido por uma única infiltração intra-articular de iodoacetato de sódio (solução 2 mg), injetada através do ligamento patelar da articulação dos animais previamente anestesiados. Os animais foram distribuídos em três grupos: grupo controle, grupo peptídeo de colágeno e grupo colágeno hidrolisado. O tratamento foi realizado por 30 dias com a administração via oral do peptídeo de colágeno ou do colágeno hidrolisado. Posteriormente, foi realizada a eutanásia dos experimentos e seguiu-se para o estudo das alterações condrais articulares. Os resultados foram avaliados conforme contagem de condrócitos por cluster e através da avaliação histológica segundo Pritzker et al. Resultados Ao observar os estágios de lesão, não foi observada significância estatística entre os grupos controle, colágeno hidrolisado e peptídeo de colágeno (p = 0,11). Ao observar os escores, houve significância estatística na comparação do grupo tratado com colágeno hidrolisado e o grupo peptídeo colágeno (p < 0,05), porém sem diferença estatística em relação ao grupo controle. Conclusão Os tratamentos propostos da lesão condral induzida com uso de colágeno hidrolisado ou peptídeos de colágeno via oral mostraram-se eficazes, com estabilização ou regressão da lesão apresentada em ratos, merecendo novas pesquisas experimentais com o intuito de compreender e melhorar o desfecho primário deste trabalho.

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Section: Methodsmentioning

confidence: 99%

Os efeitos do colágeno hidrolisado e do peptídeo de colágeno no tratamento de lesões condrais superficiais: Um estudo experimental

Santos

Xavier

Ataíde

et al. 2022

Rev Bras Ortop (Sao Paulo)

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“…The rat PB is considered a viable model in the area of microsurgical experimentation, especially due to its similarity to the human PB 4 – 8 with structural and functional equivalence in several systems 4 , 5 . For this reason, it serves the purpose of researchers who seek explanations in the experimental field for relevant phenomena observed in humans 9 , 10 , mainly when new techniques are to be used to develop basic and advanced research, especially when studying the nerve under magnification 11 .…”

Section: Introductionmentioning

confidence: 99%

“…Despite the growing interest in the application of the rat as an experimental model, a certain scarcity of details regarding its anatomorphometry of PB and its collateral nerves was observed 6 – 8 . Therefore, the purpose of this work is to study the anatomy and morphometry of the PB of rats under a high-definition videomagnification system.…”

Section: Introductionmentioning

confidence: 99%

Anatomorphometry of the brachial plexus under high-definition system: an experimental study in rats

et al. 2022

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Purpose: To study the anatomorphometry of the plexus brachialis (PB) of rats under a high-definition video system. Methods: Ten male Wistar rats discarded from other research that did not interfere in the morphology of the animal, respecting the principle of reduction, were used. All animals were submitted to the same protocol. Initially, the cervical region was shaved. The animals were placed in a dorsal position. A single elbow-to-elbow incision was performed and dissection started at the deltopectoral sulcus. The procedures were performed under a video system. To measure the structures, the Image J software was used. Results: All the PB evaluated originated from the C5-T1 spinal nerves. C5 and C6 converged to form the truncus superior, the root of C7 originated the truncus medius, and the confluence of C8 and T1 originated the truncus inferior. It was found the union of C7, C8, and T1 to form truncus inferomedialis instead of separate medial and inferior truncus. C8 (1.31 mm) was the thickest root, the truncus inferior (1.80 mm) and the nerve radialis (1.02 mm), were the thickest. Conclusions: The anatomy of the PB is comparable to humans, admitting variations. The videomagnification system is useful to perform microsurgical dissection.

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“…In the field of experimentation, the rat stands out as an important model used especially in microsurgery, due to its easy acquisition and handling, it does not require large physical spaces, and the anesthetic protocols and the physiological parameters of the species are well known, allowing for a decrease in secondary surgical complications 6 . And, mainly, this model has great anatomical similarities with the human, allowing several comparisons 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

Anatomical description of the ventral and dorsal cervical rootlets in rats: A microsurgical study

et al. 2022

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Purpose: To describe the anatomical aspects of the cervical rootlets and to quantify the number of rootlets that compose C1 to T1. Methods: Twenty male rats were used in this study. The dorsal rootlets from C1 to T1 were analyzed. To study the ventral rootlets, the posterior root avulsion was performed using a microhook, allowing exposure of the ventral roots through manipulation of the denticulate ligament and arachnoid mater. The parameters analyzed were the number of ventral and dorsal rootlets by side and level. Results: The formation of the respective spinal nerve was observed in the spinal roots the union of the ventral and dorsal roots. In four animals the C1 spinal root had no dorsal and/or ventral contribution. There is no normal pattern of numerical normality of the dorsal and ventral rootlets. The average number of fascicles per root was 4.08, with a slight superiority on the left side. There was a slight superiority of the dorsal rootlets compared to the ventral rootlets. Conclusions: This investigation was the first to study cervical rootlets in rats. In 20% of the sample studied, the dorsal root of C1 was absent mainly on the left side. There is a nonlinear numerical increase from C1 to T1 in the rootlets. There is a numerical predominance of cervical fascicles on the left side, confronting several studies related to the functional predominance of right laterality, requiring new studies that correlate these variables.

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A new anesthetic protocol to medullary nerve roots access in rats

Cited by 4 publications

References 25 publications

Os efeitos do colágeno hidrolisado e do peptídeo de colágeno no tratamento de lesões condrais superficiais: Um estudo experimental

Os efeitos do colágeno hidrolisado e do peptídeo de colágeno no tratamento de lesões condrais superficiais: Um estudo experimental

Anatomorphometry of the brachial plexus under high-definition system: an experimental study in rats

Anatomical description of the ventral and dorsal cervical rootlets in rats: A microsurgical study

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