A new analytical method for determination of ledipasvir and sofosbuvir in pharmaceutical formulations by HPLC method

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Sofosbuvir and Ledipasvir in Tablet dosage form. Chromatogram was run through Std Discovery C8 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1% OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Sofosbuvir and Ledipasvir were found to be 2.367 min and 3.436 min. %RSD of the Sofosbuvir and Ledipasvir were and found to be 0.6 and 0.5 respectively. %Recovery was obtained as 99.61% and 99.80% for Sofosbuvir and Ledipasvir respectively. LOD, LOQ values obtained from regression equations of Sofosbuvir and Ledipasvir were 0.67, 2.02 and 0.70, 2.12 respectively. Regression equation of Sofosbuvir is y = 4266.x + 7700, and y = 4861.x + 2656.of Ledipasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Development of Stability Indicating HPLC-UV Method for Determination of Process Impurities and Degradation Products in Sofosbuvir and Velpatasvir Tablets

“…Various analytical methods including spectrofluorimetry [21], UPLC [22], spectrophotometry/UPLC [23] and spectrophotometry [24] can also be used for the simultaneous analysis of SOF and VEL in pharmaceutical formulations. Stability indicating methods based on UPLC [25], HPLC-PDA [26], RP-UFLC [27] and HPLC-UV [28,29] were also reported very recently. However, these methods have some disadvantages due to low sensitivity and cannot be applied to analysis of the process impurities and degradation products.…”

Green approach for the synthesis of 3‐methyl‐1‐phenyl‐4‐((2‐phenyl‐1H‐indol 3‐yl)methylene)‐1H‐pyrazole‐5(4H)‐ones and their DNA Cleavage, antioxidant, and antimicrobial activities

“…[6] The importance of serotonin (5-hydroxytryptamine) has been recognized as a modulator of CNS neurohormonal activity. [7,8] Various biological activities like anticancer, [9,10] antirheumatoidal, [11] anti-HIV, [12] anti-inflammatory, [13] antidiabetic, [14] antihistaminic, [15] anticonvulsant, [16] antihelminthic, [17] antihypertensive, [18] antioxidant, [19] and antimicrobial [20] have been attributed to indole derivatives. Phenyl group at 2position of indole moiety enhances biological activities like antimicrobial, monoamine oxidase inhibition.…”