A neurotensin-immunoreactive pathway from the subiculum to the mammillary body in the rat

Kiyama, Hiroshi; Shiosaka, Sadao; Sakamoto, Noboru; Michel, Jean-Philippe; Pearson, John; Tohyama, Masaya

doi:10.1016/0006-8993(86)90757-2

Cited by 27 publications

(9 citation statements)

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“…4), despite previous reports of low numbers of NT-immunoreactive cell bodies in the adult (9,15,16). Labeling in the ventral subiculum was unexpected because NT-immunoreactive cell bodies have not been reported in that region of the rat at any stage of development (8,9,(15)(16)(17). Labeling was restricted to the subiculum proper, as neither the presubiculum nor the parasubiculum exhibited labeled cells.…”

Section: Resultscontrasting

confidence: 39%

“…There are indications of a similar reduction in NT-immunoreactive cells during development of the human subiculum (31). Parallel changes occur in the density of NT-immunoreactive fibers in the mammillary body, and a NT-immunoreactive pathway from subiculum to mammillary body has been identified in the neonatal rat (17) and human neonate (31). A NT-immunoreactive pathway from dorsal subiculum to anterior cingulate cortex in the adult rat has also been reported (16).…”

Section: Discussionmentioning

confidence: 64%

“…There was intense labeling of the CA1 region of hippocampus ( Fig. 2 C and D) that was unexpected in view of the reported absence of NT-immunoreactive cell bodies in this region (8,9,(15)(16)(17). This labeling occurred at all septotemporal levels of the hippocampus.…”

Section: Resultsmentioning

confidence: 93%

“…In general, the regional distribution of NT/N mRNA corresponds well with that of NT immunoreactivity. However, NT/N mRNA is abundant in the CA1 region of the hippocampus, a region where NT-immunoreactive neurons have not been detected (8,9,(15)(16)(17), even with the use of colch- FIG. 5.…”

Section: Discussionmentioning

confidence: 99%

“…The distribution of NT in the rat forebrain has been extensively mapped with immunocytochemical techniques (8,9,(15)(16)(17)(18)(19)(20)(21)(22)(23). DNA sequence analysis of cDNA and genomic clones has revealed that NT is synthesized as part of a larger precursor protein that also contains a coding domain for the NT-like peptide neuromedin N (2, 3).…”

Section: Discussionmentioning

confidence: 99%

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Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Alexander

Miller

Dorsa

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We have used in situ hybridization to determine the regional distribution of mRNA encoding the neurotensin/neuromedin N (NT/N) precursor in the forebrain of the adult male rat. Cells containing NT/N mRNA are widely distributed in the forebrain. These areas include the septum, bed nucleus of the stria terminalis, preoptic area, hypothalamus, amygdala, accumbens nucleus, caudate-putamen, and piriform and retrosplenial cortex. In general, the regional distribution of NT/N mRNA corresponds to the previously determined distribution of neurotensin-immunoreactive cell bodies; however, several notable exceptions were observed.The most striking difference occurs specifically in the CA1 region of the hippocampus, where intense labeling is associated with the pyramidal cell layer despite the reported absence of neurotensin-immunoreactive cells in this region. Analysis of microdissected tissue by S1 nuclease protection assay confirmed the abundance of authentic NT/N mRNA in CAL. A second major discrepancy between NT/N mRNA abundance and neurotensin-immunoreactivity occurs in the intensely labeled subiculum, a region that contains only scattered neurotensin-immunoreactive cells in the adult. These results suggest that, in specific regions of the forebrain, NT/N precursor is processed to yield products other than neurotensin. In addition, these results provide an anatomical basis for studying the physiological regulation of NT/N mRNA levels in the forebrain.Neurotensin (NT), a tridecapeptide with widespread distribution in the central nervous system and peripheral tissues of mammals, is one of a family of structurally related peptides with members manifesting diverse biological activities (1). The structure of the rat gene encoding NT has recently been determined (2), and the general features of the predicted NT precursor for the rat are the same as those of the predicted precursors for the dog (3) and cow (2). In each species, a precursor protein of 169-170 amino acid residues encodes both NT and neuromedin N, a peptide similar in structure to NT (4). A comparison of the sequences of the complete neurotensin/neuromedin N (NT/N) precursor from rat, dog, and cow reveals a high degree of conservation. This finding is consistent with the idea that the precursor may be processed to yield biologically active peptides that are as yet unknown.There is increasing evidence that NT functions as a neuroregulatory substance in the mammalian central nervous system (1, 5). Effects produced by NT administration in the central nervous system include hypothermia, hypotension, and antinociception. NT has also been implicated in the central regulation of anterior pituitary function (6, 7). NTimmunoreactive cell bodies are most numerous in the forebrain, with widespread distribution in limbic regions such as the septum, preoptic area, hypothalamus, and amygdala (8).Other forebrain regions, such as the dorsal subiculum, contain many NT-immunoreactive cell bodies during early stages of development, but the number of neurons with detectable...

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Section: Resultscontrasting

confidence: 39%

Section: Discussionmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Alexander

Miller

Dorsa

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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Descending projections to the mammillary nuclei in the rat, as studied by retrograde and anterograde transport of wheat germ agglutinin–horseradish peroxidase

Shibata

1989

J of Comparative Neurology

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The cells of origin and projection fields of the descending afferents to the mammillary nuclei were studied in the rat with retrograde and anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase. The subiculum projects bilaterally to the entire medial mammillary nucleus (MM) in a topographic fashion along the two axes: 1) the proximal part of the subiculum along the presubiculo-CA1 axis projects to the caudal and lateral regions of the MM whereas the more distal part of the subiculum projects to the medial region; 2) the septal part of the subiculum projects to the caudodorsal region of the MM whereas the more temporal part projects progressively to the more rostroventral regions. The ventral subiculum also projects ipsilaterally to the ventral and lateral margin of the lateral mammillary nucleus (LM). The presubiculum projects bilaterally to the dorsolateral region of the pars posterior of the MM and ipsilaterally to the LM. The infra-limbic cortex projects bilaterally to the rostrodorsal region of the MM, whereas the retrosplenial cortex (areas 29a and 29b) projects bilaterally to the medial region at the midrostrocaudal and middorsoventral levels of the MM. The nucleus of the diagonal band projects bilaterally to the caudomedial region of the MM, whereas the lateral septal nucleus projects bilaterally to the pars mediana and the mammillary fiber capsule. A part of the anterior hypothalamic area ventromedial to the fornix projects predominantly ipsilaterally to the rostroventral part of the MM, whereas other basal forebrain regions such as the bed nucleus of the stria terminalis, the medial preoptic and anterior hypothalamic areas, and the area of the tuber cinereum send fibers predominantly ipsilaterally to the mammillary fiber capsule. The results reveal a complex organization of the descending projections to the mammillary nuclei, which may reflect the complex functions of these nuclei within the limbic circuitry.

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Postnatal changes in the density and distribution of neurotensin‐like immunoreactive fibers in the mediodorsal nucleus of the thalamus in the rat

Ray

Price

1990

J of Comparative Neurology

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A previous report (Inagaki et al., Brain Res. 260:143-146, '83) suggested that the peptide neurotensin is contained in neurons of the piriform cortex that project to the mediodorsal thalamic nucleus (MD) in young rats. To confirm this, we have studied the distribution of neurotensin-like immunoreactive (NTIR) fibers in MD during development, using three antisera directed at different parts of the neurotensin molecule (Emson et al., J. Neurochem. 38:992-999, '82). In adult rats, NTIR fibers in MD are sparse. They are located mostly at the medial edge of MD and in the adjacent midline thalamic nuclei, with a few poorly stained NTIR fibers in the central part of MD. In contrast, during the first postnatal week, both the medial and central portions of MD stain heavily for neurotensin. The density of NTIR fibers in MD then progressively decreases until the density typical of adult rats is reached, at about 5 weeks. Changes in the distribution of NTIR fibers in MD also occur. In 7-day-old rats, the patches of NTIR fibers in the medial and central parts of MD are contiguous, but by 10 days a sparsely immunoreactive zone forms between them. With maturation, this zone enlarges as the density of neurotensin staining decreases, until the medial contingent of NTIR fibers reaches its adult position at the medial edge of MD. From a comparison of the distribution of NTIR cells with that of cells that can be retrogradely labeled from MD or the midline thalamus, the probable source of the NTIR fibers to the central part of MD is in the deep layer of the piriform cortex, while the NTIR fibers to the medial edge of MD and the midline nuclei may arise from the preoptic region and the medial amygdala. In neonatal rats, neurons are found in the piriform cortex, the preoptic region, and the medial amygdala, which can be double-labeled both for neurotensin and with a retrograde tracer injected into MD and the midline thalamus. Projections of the preoptic region to the thalamus have a distribution similar to that of the medial population of NTIR fibers, whereas the distribution of piriform cortical afferents in central MD matches the central patch of NTIR fibers.

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A neurotensin-immunoreactive pathway from the subiculum to the mammillary body in the rat

Cited by 27 publications

References 4 publications

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Distribution of neurotensin/neuromedin N mRNA in rat forebrain: unexpected abundance in hippocampus and subiculum.

Descending projections to the mammillary nuclei in the rat, as studied by retrograde and anterograde transport of wheat germ agglutinin–horseradish peroxidase

Postnatal changes in the density and distribution of neurotensin‐like immunoreactive fibers in the mediodorsal nucleus of the thalamus in the rat

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