A neurochemical characterisation of the golden hamster myenteric plexus

Toole, Leah; Belai, Abebech; Burnstock, Geoffrey

doi:10.1007/s004410051008

Cited by 24 publications

(17 citation statements)

References 20 publications

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“…In this way, the neurons having this enzyme are highlighted from the others by the blue color of the formed salt. Neurons stained with this technique possess the enzyme nitric oxide synthase (NOS) co-localized with the NADPH-diaphorase (Young et al, 1992;Toole et al, 1998). NOS converts the aminoacid arginine to nitric oxide (NO).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency

Araújo¹,

Sant’Ana

Molinari

et al. 2006

Int. J. Morphol.

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Quantitative study of the myenteric plexus of the descending colon of young rats subjected to intense protein deficiency. Int. J. Morphol., 24(4):591-597, 2006. SUMMARY:We studied the effects of a severely hypoproteic diet on the quantitative aspects of the myenteric plexus of the descending colon of young rats. Eighteen rats were divided into two groups, one of them being fed with a chow having 26% protein (control) and the other with a chow having 4% protein, balanced for minerals and vitamins, during 12 weeks. The whole-mounts of the descending colon had their myenteric neurons stained either with Giemsa or NADPH diaphorase. The rats from the experimental group had deficits of body weight (54.23%) and area of the descending colon (48.14%); additionally, we observed that there was no alteration in the total number of neurons of the colon, but a decrease in the number of NADPH-diaphorase positive neurons (37.80%). The implications of these results concerning the priority that some cellular types may have when nutrients are less available are discussed.

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Section: Discussionmentioning

confidence: 99%

Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency

Araújo¹,

Sant’Ana

Molinari

et al. 2006

Int. J. Morphol.

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“…Although it does not stain all the neuronal population, the histochemistry of the NADPH-d have been largely employed in the last years, because several studies indicate that the neurons which it evidences express the enzyme nitric oxide synthase (NOS) 23,24 , and thus may use nitric oxide as a neurotransmitter, being relaxation-promoting neurons, or inhibitory interneurons of the myenteric plexus 12 . The coexistence of vasoactive intestinal peptide (VIP) and NOS (evidenced by the NADPH-d) in neurons of the myenteric plexus suggests that nitric oxide (NO) and VIP can act as inhibitory, nonadrenergic noncholinergic (NANC) neurotransmitters 24,25 .…”

Section: Discussionmentioning

confidence: 99%

“…The coexistence of vasoactive intestinal peptide (VIP) and NOS (evidenced by the NADPH-d) in neurons of the myenteric plexus suggests that nitric oxide (NO) and VIP can act as inhibitory, nonadrenergic noncholinergic (NANC) neurotransmitters 24,25 . Recently it was found that all the neurons immunoreactive for VIP are NADPH-d-positive, but most of the NADPH-dpositive neurons are not immunoreactive for VIP 24 .…”

Section: Discussionmentioning

confidence: 99%

Regional differences in the number and type of myenteric neurons of the ileum of rats: a comparison of techniques of the neuronal evidentiation

Miranda-Neto

Molinari

Natali

et al. 2001

Arq. Neuro-Psiquiatr.

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-We carried out this study with the purpose of analyzing the density of neurons of the myenteric plexus in the mesenteric, intermediate and antimesenteric regions of the ileum of rats. We conclude that there is a variation of neuronal density around the intestinal circumference and this fact independs on the technique used to stain the neurons, and that in a single region the neuronal density varies with the technique employed. We also call attention for the identification of the site were countings were carried out, so that the results of research in this area are not compromised.KEY WORDS: myenteric plexus, Giemsa, NADPH-diaphorase, NADH-diaphorase, acetylcholinesterase, rat, ileum.Diferenças regionais no número e tipo de neurônios mientéricos do íleo de ratos: comparação entre técnicas de evidenciação neuronal RESUMO -Realizamos este estudo com o objetivo de analisar a densidade de neurônios do plexo mientérico nas regiões mesentérica, intermediária e antimesentérica do íleo de ratos. Empregamos preparados de membrana corados por 4 diferentes técnicas. Através de contagens sob microscópio óptico em uma área de 8,96 mm 2 encontramos com as técnicas de Giemsa, histoquímica da NADH-diaforase, NADPH-diaforase e acetilcolinesterase, respectivamente as seguintes médias neuronais: região mesentérica 2144, 40±161,05, 1657,80±88,23, 473,80±19,62, 905,25±22,40; região intermediaria 1790,60±128,24, 1265,20±141,17, 371,30±27,84, 770,25±33,12; região antimesentérica 1647,0±76,67, 981,80±68,04, 298,50±22,75, 704,50±69,38. Concluimos que há uma variação na densidade neuronal ao redor da circunferência intestinal, e este fato independe da técnica utilizada para marcar os neurônios, e que em uma mesma região, a densidade neuronal varia com a técnica utilizada. Também chamamos a atenção para que seja indicado o local onde as quantificações serão realizadas, para que não haja comprometimento dos resultados em pesquisa nesta área.

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“…In the hamster ileum, immunoreactive cell body and nerve fibers for NO synthase and SP in the myenteric plexus have been also identified, and these immunoreactive neurons densely distributed throughout the ileum [21]. These observations support a possibility that nitrergic neurons interact with tachykininergic neurons.…”

Section: Discussionmentioning

confidence: 57%

Nitrergic Inhibition of Tachykininergie Neuro-Muscular Transmission via Cyclic GMP in the Hamster Ileum

Matsuyama

Unno

Komori

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The present study was designed to explore the inhibitory mechanism by nitric oxide (NO) of the tachykininergic neuro-muscular transmissions in the hamster ileum. In the presence of guanethidine (1 µM), atropine (0.5 µM), nifedipine (0.1 µM) and apamin (100 nM), electrical field stimuli (EFS; 0.5 ms duration, 15 V) evoked non-adrenergic, non-cholinergic excitatory junction potentials (EJPs) in circular smooth muscle cells. The EJPs were markedly inhibited by the tachykinin NK1 receptor antagonists 9 ]-SP(4-11) (3 µM). Both the NO synthase inhibitor N G -nitro-L-arginine methyl ester (L-NAME, 200 µM) and the soluble guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ, 10 µM), did not affect on the resting membrane potentials, but enhanced the tachykininergic EJPs. In the presence of L-NAME (200 µM), exogenously applied NO (10 µM) and the membrane permeable analogue of guanosine 3',5'-cyclic monophosphate (cGMP), 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP, 3 mM), significantly inhibited the tachykininergic EJPs. Application of EFS (0.5 msec duration, 15 V) with trains of 20 pulses at 20 Hz increased amount of released substance P (SP). The release of SP was further increased by the treatment of L-NAME or ODQ, but markedly reduced by exogenously applied NO and 8-Br-cGMP. These results suggest that the endogenous NO may inhibit the tachykininergic neuro-muscular transmissions by the decrease of SP release from the tachykininergic neurons, possibly through a guanylate cyclasecGMP-dependent mechanism in the hamster ileum.KEY WORDS: excitatory junction potential, nitric oxide, small intestine, smooth muscle, substance P.J. Vet. Med. Sci. 73(4): 453-458, 2011 In mammalian gastrointestinal tract, nitric oxide (NO) mediates inhibitory regulation of gut motility [5]. In the hamster small intestine, it has been reported that NO released from enteric neurons act on smooth muscle cells to open apamin-sensitive, Ca 2+ -activated K + channels, resulting in membrane hyperpolarization and muscle relaxation [13]. In addition to such direct action on smooth muscles, NO has been proposed to decrease intestinal motilities by inhibiting the release of excitatory neurotransmitters [ 3 , 27, 28].In the guinea-pig ileum, NO inhibits muscle contractions evoked by tachykininergic nerve stimulation [ 3 , 2 8 ] . This effect may be partly brought about by the inhibitory action of NO on the smooth muscles. However, it is unclear whether NO also act on the tachykininergic neurons and affect neurotransmitter release.The present study was therefore designed to explore the mechanisms of inhibitory regulation by nitrergic neurons of the tachykininergic neuro-muscular transimission. Since the nitrergic neuron is predominant inhibitory neurons and tachykininergic excitatory neurotransmission is present in the hamster ileum [2, 13, 14], we used this preparation and measured excitatory junction potentials and amount of released substance P (SP) elicited by enteric nerve stimulation using microelectro...

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A neurochemical characterisation of the golden hamster myenteric plexus

Cited by 24 publications

References 20 publications

Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency

Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency

Regional differences in the number and type of myenteric neurons of the ileum of rats: a comparison of techniques of the neuronal evidentiation

Nitrergic Inhibition of Tachykininergie Neuro-Muscular Transmission via Cyclic GMP in the Hamster Ileum

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