A near-infrared emission fluorescent probe with multi-rotatable moieties for highly sensitive detection of mitochondrial viscosity in an inflammatory cell model

Ma, Yanyan; Zhao, Yuping; Guo, Rui; Zhu, Linlin; Lin, Weiying

doi:10.1039/c8tb02083c

Cited by 56 publications

(21 citation statements)

References 31 publications

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“…Based on the excellent imaging properties and photostability of DSL1 (Supporting Information Figure S12), we chose DSL1 as a representative luminophore to further clarify the advantages of DSLs in bioimaging, by comparing with classic monomer-dependent emission luminophores (Rh 6G) and aggregate-dependent emission luminophores (TPE1 and TPE2). [25][26][27][28] As shown in Supporting Information Figure S13, DSL1-loaded cells showed stronger fluorescence than TPE1 and TPE2-loaded cells under the same concentrations, suggesting its better imaging effects. In addition, with the increased incubation concentrations of these luminophores, the fluorescence in Rh 6G-incubated cells (or tissues) showed a tendency of first increasing and subsequently decreasing, and sharply increase only in high concentration of TPE1-incubated cells or tissues (Supporting Information Figure S14).…”

Section: Page 9 Of 26mentioning

confidence: 91%

An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra, Large Stokes Shift, and Activatable Fluorescence for High-Contrast Imaging

Liu

Teng

et al. 2022

CCS Chem

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Developing dual-state luminophores (DSLs) with strong fluorescence both in monomer and aggregate state is of great necessary but still a huge challenge, because most of current luminophores are either aggregationinduced emission or aggregation-caused quenching molecules. Moreover, limited by the structural conservation of the few existing DSLs, there is not enough response sites that can be used to customize various activatable fluorescent probes for specific molecular imaging. Herein, we engineered a general integration strategy for fabricating such DSLs with excellent photophysical properties, eg. tunable spectra, large Stokes shift (> 170 nm), and achievable near-infrared emission, which shows great potential in high-contrast imaging.Importantly, the DSLs can be used as a universal platform for probe customization due to their activatable fluorescence through protection-deprotection of phenolic hydroxyl group. Based on this, a near-infrared fluorescent probe (DSL-Gal) was developed for sensing of β-galactosidase in solutions, senescent cells, and liver metastases with high contrast, further confirmed the superiority and universal feasibility of DSLs in probe design. The integration strategy may provide a novel approach for other DSLs generation and our DSLs may have great potential for bioimaging.

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Section: Page 9 Of 26mentioning

confidence: 91%

An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra, Large Stokes Shift, and Activatable Fluorescence for High-Contrast Imaging

Liu

Teng

et al. 2022

CCS Chem

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“…Probe 2 with a large Stokes shift and viscosity sensitivity was used to detect mitochondrial viscosity in living cells, zebra fish, and living mice. By assembly of tetraphenylethylene (TPE) and an indole salt moiety, a near‐infrared emission fluorescent probe 3 for viscosity detection in mitochondria of inflammatory cell was developed [30] . Probe 3 showed a large Stokes shift (190 nm) and excellent sensitivity and selectivity to viscosity in near‐infrared emission (650 nm).…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

101

115

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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“…Generally, the existing models for mitochondrial viscosity studies could be classified into two categories. The first kind is the ionophores (monensin or nystatin), which could induce mitochondrial dysfunction by destroying the structure of mitochondria, leading to the viscosity variation. , The other kind is the lipopolysaccharide (LPS), which has been confirmed to increase the viscosity by producing inflammation. , Limited disease models prevent us from delving into the relationship between disease and viscosity. To address this issue, more disease models have been urgently developed for studies aimed at investigating biological processes and promoting fundamental research in mitochondrial viscosity associated diseases.…”

mentioning

confidence: 99%

Visualization of Mitochondrial Viscosity in Inflammation, Fatty Liver, and Cancer Living Mice by a Robust Fluorescent Probe

2019

Self Cite

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Elucidating the internal relationship between diseases and mitochondrial viscosity remains a great challenge due to the lack of the studies on multidisease living animals. So far, demonstrating abnormal mitochondria viscosity in the fatty liver and tumor living mice has not been achieved. To address this critical challenge, the powerful two-photon and mitochondria-targeted fluorescence probe CBI-V was designed herein for viscosity detection with deep red emission. Utilizing the new probe CBI-V, the mitochondrial viscosity alteration in living systems caused by monensin or nystatin has been monitored sensitively and selectively in real time. Moreover, the probe is capable of imaging mitochondrial viscosity in the inflammatory models at the cell, zebrafish, and mice level. Importantly, the visualization of mitochondrial viscosity has been achieved in both fatty liver and tumor living mice for the first time. This work not only advances the study of the relationship between disease and mitochondrial viscosity but also opens up an efficient way for diagnosing mitochondrial viscosity related diseases.

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A near-infrared emission fluorescent probe with multi-rotatable moieties for highly sensitive detection of mitochondrial viscosity in an inflammatory cell model

Abstract: We have designed a new near-infrared emission fluorescent probe with multi-rotatable moieties for the imaging of mitochondrial viscosity in an inflammatory cell model.

Cited by 56 publications

References 31 publications

An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra, Large Stokes Shift, and Activatable Fluorescence for High-Contrast Imaging

An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra, Large Stokes Shift, and Activatable Fluorescence for High-Contrast Imaging

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Visualization of Mitochondrial Viscosity in Inflammation, Fatty Liver, and Cancer Living Mice by a Robust Fluorescent Probe

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