A natural polyphenol-functionalized chitosan/gelatin sponge for accelerating hemostasis and infected wound healing

Abstract

Natural polymers have been particularly appealing for constructing hemostatic materials/devices, while it is still desirable to develop new natural polymer-based biomaterials with balanced hemostatic and wound healing performance. In this...

A Janus Gelatin Sponge with a Procoagulant Nanoparticle-Embedded Surface for Coagulopathic Hemostasis

“…Different from C-GS with a uniform lamellar-structure interior morphology, S-GS exhibited a uniform three-dimensional network with high porosity due to the freeze-drying of foaming gelatin solution. 33,34 On the contrary, S-GS displayed an obvious Janus interior morphology due to the incorporation of P 65 nanoparticles on the bottom of foaming gelatin solution. In addition, the bottom-/top-surface images further support the distinct structure of J-ZGS: the bottom surface was dense and of low porosity due to the dual cross-linking of gelatin chains/ zein nanoparticles (Figure 4a), while the top surface consisting of cross-linked gelatin chains was less dense and of high porosity (which is almost the same with that of S-GS, Figure S5a,b, Supporting Information).…”

“…32,33 As shown in Figure 1, the optimal P 65 was deposited/embedded on the surface during the processing of a self-prepared gelatin sponge (S-GS), in order to maximize the application of such potent hemostatic nanoparticles (located on the blood contact side of J-ZGS) and to avoid the risks of the intravascular thrombus (cross-linked with the gelatin network with glutaraldehyde). Of note, the viscous solution of amphiphilic gelatin was stirred vigorously using an egg whisk, 33 to produce a self-foaming solution for preparing S-GS and J-ZGS under a "green" condition (without an extra foaming agent).…”

“…All these results demonstrated that J-ZGS and S-GS achieved the similarly high liquidabsorption capacity due to the freeze-drying of cross-linked/ foaming gelatin solution. 33,34 In Vitro Biocompatibility and Hemostatic Assays of the Janus Sponge. As a prerequisite for potential medical sponges, the in vitro biocompatibility of J-ZGS was confirmed by blood hemolysis and cell viability assays.…”

“…Commercial gelatin sponge (C-GS), prepared by the freeze-drying of gelatin solution and glutaraldehyde (as the cross-linking agent), is a widely used hemostatic agent for clinical application, which benefits from high biosafety/biodegradability but is also limited by unsatisfactory performance in serious bleeding (i.e., hemorrhage). , As shown in Figure , the optimal P 65 was deposited/embedded on the surface during the processing of a self-prepared gelatin sponge (S-GS), in order to maximize the application of such potent hemostatic nanoparticles (located on the blood contact side of J-ZGS) and to avoid the risks of the intravascular thrombus (cross-linked with the gelatin network with glutaraldehyde). Of note, the viscous solution of amphiphilic gelatin was stirred vigorously using an egg whisk, to produce a self-foaming solution for preparing S-GS and J-ZGS under a “green” condition (without an extra foaming agent).…”

Electroactive nanofibrous membrane with temperature monitoring for wound healing

“…Severe inammatory response and inltration of inammatory cells can delay wound healing. 53,54 The wound site skin was taken for HE staining on days 6 and 9 to observe the inammatory cell inltration (Fig. 7a and c).…”