A Janus Gelatin Sponge with a Procoagulant Nanoparticle-Embedded Surface for Coagulopathic Hemostasis

Abstract

Apart from the wide and safe application of natural polymerbased hemostatic materials/devices, it is still desirable to develop new types of hemostatic materials that can achieve both potent coagulopathic hemostasis and a facile preparation process. In this work, one Janus gelatin sponge (J-ZGS) is readily constructed for both coagulation-dependent and coagulopathic hemostasis by embedding zein nanoparticles on the surface of a self-prepared gelatin sponge (S-GS): zein nanoparticles were facilely prepared by an antisolvent method to achieve procoagulant blood−material interactions, while S-GS was prepared by freeze-drying a foaming gelatin solution. Due to the distinct secondary structure, the optimal zein nanoparticles possessed a higher in vitro hemostatic property than the pristine zein powder and other nanoparticles, the underlying mechanism of which was revealed as the superior RBC/platelet adhesion property in the presence/absence of plasma proteins. Compared with S-GS and a commercial gelatin sponge, J-ZGS achieved a significantly higher in vitro hemostatic property and similarly good blood compatibility/cytocompatibility. Moreover, in vivo artery-injury models confirmed the outstanding hemostatic performance of J-ZGS under both coagulation-dependent and coagulopathic conditions. Our work offers an appealing approach for developing potent hemostatic sponges from natural polymer-based nanoparticles that could be further extended to versatile hemostatic materials for coagulopathic hemostasis.

Facile preparation of injectable, thermosensitive, and physically cross-linked hemostatic hydrogel with rapid gelation and a robust network

“…In recent years, hydrogels have been used as hemostats and tissue sealants instead of traditional sutures and staples, due to their excellent performance in sealing wounds and halting bleeding. 3 Various materials have been developed to fabricate hemostatic hydrogels, such as cellulose, 4,5 other plant polysaccharides, 6–8 gelatin (G)/collagen, 9–11 chitosan, 12,13 fibrinogen, 14 hyaluronic acid (HA), 15 and so on. In particular, gelatin/hyaluronic acid (G/HA) based biomimetic hemostatic hydrogels have emerged as promising hemostats to stop bleeding and promote wound healing due to their perfect structural and mechanical similarity to the extracellular matrix (ECM) of biological tissues.…”