A native variant of Chrysodeixis chalcites nucleopolyhedrovirus: The basis for a promising bioinsecticide for control of C. chalcites on Canary Islands’ banana crops

Bernal, Alexandra; Williams, Trevor; Hernández‐Suárez, Estrella; Carnero, A.; Caballero, Primitivo; Simón, Oihane

doi:10.1016/j.biocontrol.2013.08.006

Cited by 16 publications

(46 citation statements)

References 40 publications

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“…C. chalcites larvae were obtained from pupae received from the Instituto Canario de Investigaciones Agrarias (ICIA), Tenerife, Spain, in 2007 and refreshed periodically with pupae from the Canary Islands. Previous quantitative real-time PCR (qPCR) studies indicated that the insect colony did not harbor an inapparent nucleopolyhedrovirus infection (31). Larvae were reared at 25 Ϯ 1°C and 70% Ϯ 5% humidity with a photoperiod of 16:8 (light-dark) on a semisynthetic diet described by Greene et al (32).…”

Section: Methodsmentioning

confidence: 99%

“…OBs of the different clones were purified from dead larvae, and DNA was purified as previously described (31). For restriction endonuclease analysis, viral DNA was mixed with BglII, as the enzyme allowed clear discrimination between the different ChchSNPV isolates (31).…”

Section: Methodsmentioning

confidence: 99%

“…For restriction endonuclease analysis, viral DNA was mixed with BglII, as the enzyme allowed clear discrimination between the different ChchSNPV isolates (31).…”

Section: Methodsmentioning

confidence: 99%

“…In the Canary Islands, Spain, C. chalcites larvae can cause up to 30% production loss in banana crops (28). To date, different geographical C. chalcites singlenucleocapsid nucleopolyhedrovirus (ChchSNPV) strains have been described: one from the Netherlands (ChchSNPV-NL) (27,29); another from Almería, Spain (ChchNPV-SP1) (30); and five from Tenerife, Canary Islands, Spain (ChchSNPV-TF1 to -TF5) (31). The ChchSNPV-TF1 isolate was recently selected for further development as a bioinsecticide for protection of banana crops due to its high pathogenicity and speed of kill against an insect population from the Canary Islands (31).…”

mentioning

confidence: 99%

“…To date, different geographical C. chalcites singlenucleocapsid nucleopolyhedrovirus (ChchSNPV) strains have been described: one from the Netherlands (ChchSNPV-NL) (27,29); another from Almería, Spain (ChchNPV-SP1) (30); and five from Tenerife, Canary Islands, Spain (ChchSNPV-TF1 to -TF5) (31). The ChchSNPV-TF1 isolate was recently selected for further development as a bioinsecticide for protection of banana crops due to its high pathogenicity and speed of kill against an insect population from the Canary Islands (31). The aims of the present study were, first, to evaluate the genetic structure of the ChchSNPV-TF1 wild-type isolate and, second, to determine the outcome of genotype interactions for the insecticidal phenotype of genotype mixtures by testing different occlusion body (OB) and cooccluded mixtures.…”

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confidence: 99%

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A Chrysodeixis chalcites Single-Nucleocapsid Nucleopolyhedrovirus Population from the Canary Islands Is Genotypically Structured To Maximize Survival

Bernal

Simón

Williams

et al. 2013

Appl Environ Microbiol

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A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus wild-type isolate from the Canary Islands, Spain, named ChchSNPV-TF1 (ChchTF1-wt), appears to have great potential as the basis for a biological insecticide for control of the pest. An improved understanding of the genotypic structure of this wild-type strain population should facilitate the selection of genotypes for inclusion in a bioinsecticidal product. Eight genetically distinct genotypes were cloned in vitro: ChchTF1-A to ChchTF1-H. Quantitative real-time PCR (qPCR) analysis confirmed that ChchTF1-A accounted for 36% of the genotypes in the wild-type population. In bioassays, ChchTF1-wt occlusion bodies (OBs) were significantly more pathogenic than any of the component single-genotype OBs, indicating that genotype interactions were likely responsible for the pathogenicity phenotype of wild-type OBs. However, the wild-type population was slower killing and produced higher OB yields than any of the single genotypes alone. These results strongly suggested that the ChchTF1-wt population is structured to maximize its transmission efficiency. Experimental OB mixtures and cooccluded genotype mixtures containing the most abundant and the rarest genotypes, at frequencies similar to those at which they were isolated, revealed a mutualistic interaction that restored the pathogenicity of OBs. In OB and cooccluded mixtures containing only the most abundant genotypes, ChchTF1-ABC, OB pathogenicity was even greater than that of wild-type OBs. The ChchTF1-ABC cooccluded mixture killed larvae 33 h faster than the wild-type population and remained genotypically and biologically stable throughout five successive passages in vivo. In conclusion, the ChchTF1-ABC mixture shows great potential as the active ingredient of a bioinsecticide to control C. chalcites in the Canary Islands.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…For restriction endonuclease analysis, viral DNA was mixed with BglII, as the enzyme allowed clear discrimination between the different ChchSNPV isolates (31).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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