Efficacy of an alphabaculovirus-based biological insecticide for control of<i>Chrysodeixis chalcites</i>(Lepidoptera: Noctuidae) on tomato and banana crops

Simón, Oihane; Bernal, Alexandra; Williams, Trevor; Carnero, A.; Hernández‐Suárez, Estrella; Muñoz, Delia; Caballero, Primitivo

doi:10.1002/ps.3969

Cited by 14 publications

(20 citation statements)

References 39 publications

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“…The insecticidal efficacy of the ChchNPV-TF1 variant was compared in greenhouse and open-field conditions, as ~65% of the banana production is produced in open-field conditions in the Canary Islands [ 1 ], and we had not previously performed such a comparison [ 10 ]. As greenhouse conditions tend to be more stable than those of open-field crops [ 28 , 29 ], the harsher conditions of open-field crops might have implications for the quantities of viral OBs required for effective control or may affect the persistence of viral OB treatments on crop foliage [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies had indicated that 10 9 OBs/l might be sufficient to protect banana plants from C . chalcites feeding damage under greenhouse conditions [ 10 ]. This concentration of viral OBs is similar to that reported in other baculoviruses used as biological insecticides, such as those of nucleopolyhedroviruses applied for the control of Spodoptera spp.…”

Section: Discussionmentioning

confidence: 99%

“…For field trials, ChchNPV-TF1 isolate OBs were produced by inoculating fifth and sixth instar laboratory-reared C . chalcites larvae with 5.00x10 7 OBs/ml or 9.02x10 8 OBs/ml, respectively [ 5 , 10 ]. OB inocula were suspended in 10% sucrose solution and 0.001% Fluorella blue food dye, and fed to larvae using the droplet feeding method [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…To determine the most appropriate concentration for ChchNPV-TF1 OB application under field conditions we followed previous studies in which three viral concentrations were evaluated to estimate the most suitable concentration for use in field assays [ 10 ]. For this, trials were performed in 2013 using young banana plants ( Musa acuminata , var.…”

Section: Methodsmentioning

confidence: 99%

“…chalcites larva collected in banana crops in southern Tenerife, was characterized and named ChchNPV-TF1 [ 5 ]. Laboratory and small-scale greenhouse trials on young banana plants indicated that the high pathogenicity and fast speed of kill of this isolate favored its development as a biological insecticide for the control of this pest [ 5 , 10 ]. Studies on other lepidopteran-nucleopolyhedrovirus pathosystems indicate that genetic diversity in the pathogen population has a marked influence on major phenotypic traits including pathogenicity, measured in terms of concentration-mortality metrics, speed of kill and production of progeny virus particles in each infected insect [ 11 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV): Natural occurrence and efficacy as a biological insecticide on young banana plants in greenhouse and open-field conditions on the Canary Islands

et al. 2017

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Chrysodeixis chalcites, an important pest of banana crops on the Canary Islands, is usually controlled by chemical insecticides. The present study aimed to evaluate the efficacy of the most prevalent isolate of the Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV, Baculoviridae) as a biological insecticide. Overall the prevalence of ChchNPV infection in C. chalcites populations was 2.3% (103 infected larvae out of 4,438 sampled), but varied from 0–4.8% on Tenerife and was usually low (0–2%) on the other islands. On Tenerife, infected larvae were present at 11 out of 17 plantations sampled. The prevalence of infection in larvae on bananas grown under greenhouse structures was significantly higher (3%) than in open-field sites (1.4%). The ChchNPV-TF1 isolate was the most abundant and widespread of four genetic variants of the virus. Application of 1.0x109 viral occlusion bodies (OBs)/l of ChchNPV-TF1 significantly reduced C. chalcites foliar damage in young banana plants as did commonly used pesticides, both in greenhouse and open-field sites. The insecticidal efficacy of ChchNPV-TF1 was similar to that of indoxacarb and a Bacillus thuringiensis (Bt)-based insecticide in one year of trials and similar to Bt in the following year of trails in greenhouse and field crops. However, larvae collected at different time intervals following virus treatments and reared in the laboratory experienced 2–7 fold more mortality than insects from conventional insecticide treatments. This suggests that the acquisition of lethal dose occurred over an extended period (up to 7 days) compared to a brief peak in larvae on plants treated with conventional insecticides. These results should prove useful for the registration of a ChchNPV-based insecticide for integrated management of this pest in banana crops on the Canary Islands.

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