A nanobody-based electrochemiluminescent immunosensor for sensitive detection of human procalcitonin

Abstract

The development of a nanobody-based electrochemiluminescent immunosensor for procalcitonin quantification is described. A highly specific and enhanced sensitivity of target detection was achieved by CdTe quantum dot encapsulated silica nanoparticle-assisted signal amplification.

Development of a fluorescent immnunochromatographic assay for the procalcitonin detection of clinical patients in China

“…Several methods have been developed to detect PCT levels in the blood, among which are chemiluminescence immunoassay, enzymelinked immunosorbent assay and Thermo Fisher SPCT-Q-semi quantitative assay [13][14][15][16]. All these methods employ the use of antibodies with high sensitivity and specificity.…”

A Dual-Coreactants Electrochemiluminescent Immunosensor for Procalcitonin Detection Based on CdS-MoS2Nanocomposites

“…The limit of detection (LOD) was 33 fg mL −1 , which was much lower than those reported previously (Table I). 7,[35][36][37][38] Compared with some reported methods, the analytical performance of the constructed ECL immunosensor was more excellent for PCT. What's more, the detection method for PCT was simple and rapid.…”

Nanobodies: Chemical Functionalization Strategies and Intracellular Applications

“…for example,t othe development of various nanobody-based high-throughput assays to identify and quantify clinically relevant biomarkers,i ncluding testosterone and proclacitonin. [55] Thef luorescent labelling of proteins has become one of the most important tools for visualizing and understanding cellular structures and intracellular processes.F luorescent proteins (FPs), like GFP,a re the most frequently used biosensors,a nd their fusion to ap rotein of interest enables the dynamic visualization of proteins of interest in living cells. [56] Even though genetic fusion to FPs is straightforward, their fluorescence properties,a sw ell as at endencyt owards photobleaching,l imit their spectroscopic use,a nd their possible impact to the biological function of the protein of interest is often underestimated.…”